Research output: Contribution to journal › Article › peer-review
Otu and Rif1 Double Mutant Enables Analysis of Satellite DNA in Polytene Chromosomes of Ovarian Germ Cells in Drosophila melanogaster. / Kolesnikova, T D; Nokhova, A R; Shatskikh, A S et al.
In: Doklady. Biochemistry and biophysics, Vol. 513, No. Suppl 1, 2024, p. S87-S91.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Otu and Rif1 Double Mutant Enables Analysis of Satellite DNA in Polytene Chromosomes of Ovarian Germ Cells in Drosophila melanogaster
AU - Kolesnikova, T D
AU - Nokhova, A R
AU - Shatskikh, A S
AU - Klenov, M S
AU - Zhimulev, I F
N1 - Part of the work related to the morphological analysis of the chromosomes of nurse cells was funded by the Russian Science Foundation (grant no. 19-14-00051-P). Part of the work related to in situ hybridization was funded by the Russian Science Foundation (grant no. 19-14-00382). © 2023. Pleiades Publishing, Ltd.
PY - 2024
Y1 - 2024
N2 - Polytene chromosomes in Drosophila serve as a classical model for cytogenetic studies. However, heterochromatic regions of chromosomes are typically under-replicated, hindering their analysis. Mutations in the Rif1 gene lead to additional replication of heterochromatic sequences, including satellite DNA, in salivary gland cells. Here, we investigated the impact of the Rif1 mutation on heterochromatin in polytene chromosomes formed in ovarian germ cells due to the otu gene mutation. By the analysis of otu11; Rif11 double mutants, we found that, in the presence of the Rif1 mutation, ovarian cells undergo additional polytenization of pericentromeric regions. This includes the formation of large chromatin blocks composed of satellite DNA. Thus, the effects of the Rif1 mutation are similar in salivary gland and germ cells. The otu11; Rif11 system opens new possibilities for studying factors associated with heterochromatin during oogenesis.
AB - Polytene chromosomes in Drosophila serve as a classical model for cytogenetic studies. However, heterochromatic regions of chromosomes are typically under-replicated, hindering their analysis. Mutations in the Rif1 gene lead to additional replication of heterochromatic sequences, including satellite DNA, in salivary gland cells. Here, we investigated the impact of the Rif1 mutation on heterochromatin in polytene chromosomes formed in ovarian germ cells due to the otu gene mutation. By the analysis of otu11; Rif11 double mutants, we found that, in the presence of the Rif1 mutation, ovarian cells undergo additional polytenization of pericentromeric regions. This includes the formation of large chromatin blocks composed of satellite DNA. Thus, the effects of the Rif1 mutation are similar in salivary gland and germ cells. The otu11; Rif11 system opens new possibilities for studying factors associated with heterochromatin during oogenesis.
KW - Animals
KW - Carrier Proteins/genetics
KW - Chromosomes
KW - DNA Replication
KW - DNA, Satellite/genetics
KW - Drosophila/genetics
KW - Drosophila melanogaster/genetics
KW - Drosophila Proteins/genetics
KW - Germ Cells
KW - Heterochromatin/genetics
KW - Polytene Chromosomes/genetics
KW - Rif1
KW - heterochromatin
KW - satellite DNA
KW - polytene chromosomes
KW - under-replication
KW - pseudonurse cells
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85184919974&origin=inward&txGid=ca04966e4026ccc7640e7566382d60a3
UR - https://www.mendeley.com/catalogue/b00dfdbd-b564-3e17-9b5e-16e39e92e337/
U2 - 10.1134/S160767292360046X
DO - 10.1134/S160767292360046X
M3 - Article
C2 - 38337102
VL - 513
SP - S87-S91
JO - Doklady Biochemistry and Biophysics
JF - Doklady Biochemistry and Biophysics
SN - 1607-6729
IS - Suppl 1
ER -
ID: 60461281