Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Nuclear apoptotic volume decrease in individual cells: Confocal microscopy imaging and kinetic modeling. / Khalo, Irina V.; Konokhova, Anastasiya I.; Orlova, Darya Y. и др.
в: Journal of Theoretical Biology, Том 454, 07.10.2018, стр. 60-69.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
}
TY - JOUR
T1 - Nuclear apoptotic volume decrease in individual cells: Confocal microscopy imaging and kinetic modeling
AU - Khalo, Irina V.
AU - Konokhova, Anastasiya I.
AU - Orlova, Darya Y.
AU - Trusov, Konstantin V.
AU - Yurkin, Maxim A.
AU - Bartova, Eva
AU - Kozubek, Stanislav
AU - Maltsev, Valeri P.
AU - Chernyshev, Andrei V.
N1 - Copyright © 2018 Elsevier Ltd. All rights reserved.
PY - 2018/10/7
Y1 - 2018/10/7
N2 - The dynamics of nuclear morphology changes during apoptosis remains poorly investigated and understood. Using 3D time-lapse confocal microscopy we performed a study of early-stage apoptotic nuclear morphological changes induced by etoposide in single living HepG2 cells. These observations provide a definitive evidence that nuclear apoptotic volume decrease (AVD) is occurring simultaneously with peripheral chromatin condensation (so called “apoptotic ring”). In order to describe quantitatively the dynamics of nuclear morphological changes in the early stage of apoptosis we suggest a general molecular kinetic model, which fits well the obtained experimental data in our study. Results of this work may clarify molecular mechanisms of nuclear morphology changes during apoptosis.
AB - The dynamics of nuclear morphology changes during apoptosis remains poorly investigated and understood. Using 3D time-lapse confocal microscopy we performed a study of early-stage apoptotic nuclear morphological changes induced by etoposide in single living HepG2 cells. These observations provide a definitive evidence that nuclear apoptotic volume decrease (AVD) is occurring simultaneously with peripheral chromatin condensation (so called “apoptotic ring”). In order to describe quantitatively the dynamics of nuclear morphological changes in the early stage of apoptosis we suggest a general molecular kinetic model, which fits well the obtained experimental data in our study. Results of this work may clarify molecular mechanisms of nuclear morphology changes during apoptosis.
KW - 3D time lapsed confocal microscopy
KW - Apoptosis
KW - Apoptotic ring
KW - Chromatin condensation
KW - Kinetics
KW - Live cell analysis
KW - Mathematical modeling
KW - Nuclear volume decrease
KW - TRANSLOCATION
KW - CHROMATIN CONDENSATION
KW - DNA-BINDING
KW - PROTEIN
KW - MITOCHONDRIA
KW - DEATH
KW - Apoptosis Nuclear volume decrease
KW - ENVELOPE
KW - DYNAMICS
KW - DEGRADATION
KW - FACTOR AIF
UR - http://www.scopus.com/inward/record.url?scp=85048459790&partnerID=8YFLogxK
U2 - 10.1016/j.jtbi.2018.05.034
DO - 10.1016/j.jtbi.2018.05.034
M3 - Article
C2 - 29859212
AN - SCOPUS:85048459790
VL - 454
SP - 60
EP - 69
JO - Journal of Theoretical Biology
JF - Journal of Theoretical Biology
SN - 0022-5193
ER -
ID: 14047538