Nuclear apoptotic volume decrease in individual cells: Confocal microscopy imaging and kinetic modeling

Standard

Nuclear apoptotic volume decrease in individual cells: Confocal microscopy imaging and kinetic modeling. / Khalo, Irina V.; Konokhova, Anastasiya I.; Orlova, Darya Y. et al.

In: Journal of Theoretical Biology, Vol. 454, 07.10.2018, p. 60-69.

Research output: Contribution to journal › Article › peer-review

Harvard

Khalo, IV, Konokhova, AI, Orlova, DY, Trusov, KV, Yurkin, MA, Bartova, E, Kozubek, S, Maltsev, VP & Chernyshev, AV 2018, 'Nuclear apoptotic volume decrease in individual cells: Confocal microscopy imaging and kinetic modeling', Journal of Theoretical Biology, vol. 454, pp. 60-69. https://doi.org/10.1016/j.jtbi.2018.05.034

APA

Khalo, I. V., Konokhova, A. I., Orlova, D. Y., Trusov, K. V., Yurkin, M. A., Bartova, E., Kozubek, S., Maltsev, V. P., & Chernyshev, A. V. (2018). Nuclear apoptotic volume decrease in individual cells: Confocal microscopy imaging and kinetic modeling. Journal of Theoretical Biology, 454, 60-69. https://doi.org/10.1016/j.jtbi.2018.05.034

Vancouver

Khalo IV, Konokhova AI, Orlova DY, Trusov KV, Yurkin MA, Bartova E et al. Nuclear apoptotic volume decrease in individual cells: Confocal microscopy imaging and kinetic modeling. Journal of Theoretical Biology. 2018 Oct 7;454:60-69. doi: 10.1016/j.jtbi.2018.05.034

Author

Khalo, Irina V. ; Konokhova, Anastasiya I. ; Orlova, Darya Y. et al. / Nuclear apoptotic volume decrease in individual cells: Confocal microscopy imaging and kinetic modeling. In: Journal of Theoretical Biology. 2018 ; Vol. 454. pp. 60-69.

BibTeX

@article{90ec97cb4483458786923dc289407093,

title = "Nuclear apoptotic volume decrease in individual cells: Confocal microscopy imaging and kinetic modeling",

abstract = "The dynamics of nuclear morphology changes during apoptosis remains poorly investigated and understood. Using 3D time-lapse confocal microscopy we performed a study of early-stage apoptotic nuclear morphological changes induced by etoposide in single living HepG2 cells. These observations provide a definitive evidence that nuclear apoptotic volume decrease (AVD) is occurring simultaneously with peripheral chromatin condensation (so called “apoptotic ring”). In order to describe quantitatively the dynamics of nuclear morphological changes in the early stage of apoptosis we suggest a general molecular kinetic model, which fits well the obtained experimental data in our study. Results of this work may clarify molecular mechanisms of nuclear morphology changes during apoptosis.",

keywords = "3D time lapsed confocal microscopy, Apoptosis, Apoptotic ring, Chromatin condensation, Kinetics, Live cell analysis, Mathematical modeling, Nuclear volume decrease, TRANSLOCATION, CHROMATIN CONDENSATION, DNA-BINDING, PROTEIN, MITOCHONDRIA, DEATH, Apoptosis Nuclear volume decrease, ENVELOPE, DYNAMICS, DEGRADATION, FACTOR AIF",

author = "Khalo, {Irina V.} and Konokhova, {Anastasiya I.} and Orlova, {Darya Y.} and Trusov, {Konstantin V.} and Yurkin, {Maxim A.} and Eva Bartova and Stanislav Kozubek and Maltsev, {Valeri P.} and Chernyshev, {Andrei V.}",

year = "2018",

month = oct,

day = "7",

doi = "10.1016/j.jtbi.2018.05.034",

language = "English",

volume = "454",

pages = "60--69",

journal = "Journal of Theoretical Biology",

issn = "0022-5193",

publisher = "Academic Press Inc.",

}

RIS

TY - JOUR

T1 - Nuclear apoptotic volume decrease in individual cells: Confocal microscopy imaging and kinetic modeling

AU - Khalo, Irina V.

AU - Konokhova, Anastasiya I.

AU - Orlova, Darya Y.

AU - Trusov, Konstantin V.

AU - Yurkin, Maxim A.

AU - Bartova, Eva

AU - Kozubek, Stanislav

AU - Maltsev, Valeri P.

AU - Chernyshev, Andrei V.

PY - 2018/10/7

Y1 - 2018/10/7

N2 - The dynamics of nuclear morphology changes during apoptosis remains poorly investigated and understood. Using 3D time-lapse confocal microscopy we performed a study of early-stage apoptotic nuclear morphological changes induced by etoposide in single living HepG2 cells. These observations provide a definitive evidence that nuclear apoptotic volume decrease (AVD) is occurring simultaneously with peripheral chromatin condensation (so called “apoptotic ring”). In order to describe quantitatively the dynamics of nuclear morphological changes in the early stage of apoptosis we suggest a general molecular kinetic model, which fits well the obtained experimental data in our study. Results of this work may clarify molecular mechanisms of nuclear morphology changes during apoptosis.

AB - The dynamics of nuclear morphology changes during apoptosis remains poorly investigated and understood. Using 3D time-lapse confocal microscopy we performed a study of early-stage apoptotic nuclear morphological changes induced by etoposide in single living HepG2 cells. These observations provide a definitive evidence that nuclear apoptotic volume decrease (AVD) is occurring simultaneously with peripheral chromatin condensation (so called “apoptotic ring”). In order to describe quantitatively the dynamics of nuclear morphological changes in the early stage of apoptosis we suggest a general molecular kinetic model, which fits well the obtained experimental data in our study. Results of this work may clarify molecular mechanisms of nuclear morphology changes during apoptosis.

KW - 3D time lapsed confocal microscopy

KW - Apoptosis

KW - Apoptotic ring

KW - Chromatin condensation

KW - Kinetics

KW - Live cell analysis

KW - Mathematical modeling

KW - Nuclear volume decrease

KW - TRANSLOCATION

KW - CHROMATIN CONDENSATION

KW - DNA-BINDING

KW - PROTEIN

KW - MITOCHONDRIA

KW - DEATH

KW - Apoptosis Nuclear volume decrease

KW - ENVELOPE

KW - DYNAMICS

KW - DEGRADATION

KW - FACTOR AIF

UR - http://www.scopus.com/inward/record.url?scp=85048459790&partnerID=8YFLogxK

U2 - 10.1016/j.jtbi.2018.05.034

DO - 10.1016/j.jtbi.2018.05.034

M3 - Article

C2 - 29859212

AN - SCOPUS:85048459790

VL - 454

SP - 60

EP - 69

JO - Journal of Theoretical Biology

JF - Journal of Theoretical Biology

SN - 0022-5193

ER -

ID: 14047538