TY - JOUR

T1 - Novel carbon nanozymes with enhanced phosphatase-like catalytic activity for antimicrobial applications

AU - Nurtay, Lazzat

AU - Benassi, Enrico

AU - Nazir, Faisal

AU - Dastan, Dana

AU - Utupova, Assem

AU - Dautov, Adilet

AU - Dukenbayev, Kanat

AU - Xie, Yingqiu

AU - Pham, Tri T

AU - Fan, Haiyan

N1 - Funding This work was supported by Nazarbayev University Small Grant No. 110119FD4542 (HF), 110119FD4531 (YX), 11022021FD2924 (TTP) and 11022021FD2928 (HF). © 2023. The Author(s).

PY - 2023/5/23

Y1 - 2023/5/23

N2 - In this work, Sulfur and Nitrogen co-doped carbon nanoparticles (SN-CNPs) were synthesized by hydrothermal method using dried beet powder as the carbon source. TEM and AFM images indicated that these SN-CNPs form a round-shape ball with an approximate diameter of 50 nm. The presence of Sulfur and Nitrogen in these carbon-based nanoparticles was confirmed by FTIR and XPS analyses. These SN-CNPs were found to have strong phosphatase-like enzymatic activity. The enzymatic behavior of SN-CNPs follows the Michaelis-Menten mechanism with greater vmax and much lower Km values compared to alkaline phosphatase. Their antimicrobial properties were tested on E. coli and L. lactis, with MIC values of 63 μg mL-1 and 250 μg mL-1, respectively. SEM and AFM images of fixed and live E. coli cells revealed that SN-CNPs strongly interacted with the outer membranes of bacterial cells, significantly increasing the cell surface roughness. The chemical interaction between SN-CNPs and phospholipid modeled using quantum mechanical calculations further support our hypothesis that the phosphatase and antimicrobial properties of SN-CNPs are due to the thiol group on the SN-CNPs, which is a mimic of the cysteine-based protein phosphatase. The present work is the first to report carbon-based nanoparticles with strong phosphatase activity and propose a phosphatase natured antimicrobial mechanism. This novel class of carbon nanozymes has the potential to be used for effective catalytic and antibacterial applications.

AB - In this work, Sulfur and Nitrogen co-doped carbon nanoparticles (SN-CNPs) were synthesized by hydrothermal method using dried beet powder as the carbon source. TEM and AFM images indicated that these SN-CNPs form a round-shape ball with an approximate diameter of 50 nm. The presence of Sulfur and Nitrogen in these carbon-based nanoparticles was confirmed by FTIR and XPS analyses. These SN-CNPs were found to have strong phosphatase-like enzymatic activity. The enzymatic behavior of SN-CNPs follows the Michaelis-Menten mechanism with greater vmax and much lower Km values compared to alkaline phosphatase. Their antimicrobial properties were tested on E. coli and L. lactis, with MIC values of 63 μg mL-1 and 250 μg mL-1, respectively. SEM and AFM images of fixed and live E. coli cells revealed that SN-CNPs strongly interacted with the outer membranes of bacterial cells, significantly increasing the cell surface roughness. The chemical interaction between SN-CNPs and phospholipid modeled using quantum mechanical calculations further support our hypothesis that the phosphatase and antimicrobial properties of SN-CNPs are due to the thiol group on the SN-CNPs, which is a mimic of the cysteine-based protein phosphatase. The present work is the first to report carbon-based nanoparticles with strong phosphatase activity and propose a phosphatase natured antimicrobial mechanism. This novel class of carbon nanozymes has the potential to be used for effective catalytic and antibacterial applications.

KW - Carbon-based nanoparticles

KW - Enzymatic activity

KW - Minimum inhibitory concentration

KW - Phosphatase and antimicrobial properties

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85160015356&origin=inward&txGid=df9b4eb871af88e128550c44392acfd5

UR - https://www.mendeley.com/catalogue/305d34d5-0173-3983-aa14-91bd9bbe7556/

U2 - 10.1186/s11671-023-03856-y

DO - 10.1186/s11671-023-03856-y

M3 - Article

C2 - 37382706

VL - 18

JO - Discover nano

JF - Discover nano

SN - 2731-9229

IS - 1

M1 - 76

ER -