Novel carbon nanozymes with enhanced phosphatase-like catalytic activity for antimicrobial applications. / Nurtay, Lazzat; Benassi, Enrico; Nazir, Faisal et al.
In: Discover nano, Vol. 18, No. 1, 76, 23.05.2023.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Novel carbon nanozymes with enhanced phosphatase-like catalytic activity for antimicrobial applications
AU - Nurtay, Lazzat
AU - Benassi, Enrico
AU - Nazir, Faisal
AU - Dastan, Dana
AU - Utupova, Assem
AU - Dautov, Adilet
AU - Dukenbayev, Kanat
AU - Xie, Yingqiu
AU - Pham, Tri T
AU - Fan, Haiyan
N1 - Funding This work was supported by Nazarbayev University Small Grant No. 110119FD4542 (HF), 110119FD4531 (YX), 11022021FD2924 (TTP) and 11022021FD2928 (HF). © 2023. The Author(s).
PY - 2023/5/23
Y1 - 2023/5/23
N2 - In this work, Sulfur and Nitrogen co-doped carbon nanoparticles (SN-CNPs) were synthesized by hydrothermal method using dried beet powder as the carbon source. TEM and AFM images indicated that these SN-CNPs form a round-shape ball with an approximate diameter of 50 nm. The presence of Sulfur and Nitrogen in these carbon-based nanoparticles was confirmed by FTIR and XPS analyses. These SN-CNPs were found to have strong phosphatase-like enzymatic activity. The enzymatic behavior of SN-CNPs follows the Michaelis-Menten mechanism with greater vmax and much lower Km values compared to alkaline phosphatase. Their antimicrobial properties were tested on E. coli and L. lactis, with MIC values of 63 μg mL-1 and 250 μg mL-1, respectively. SEM and AFM images of fixed and live E. coli cells revealed that SN-CNPs strongly interacted with the outer membranes of bacterial cells, significantly increasing the cell surface roughness. The chemical interaction between SN-CNPs and phospholipid modeled using quantum mechanical calculations further support our hypothesis that the phosphatase and antimicrobial properties of SN-CNPs are due to the thiol group on the SN-CNPs, which is a mimic of the cysteine-based protein phosphatase. The present work is the first to report carbon-based nanoparticles with strong phosphatase activity and propose a phosphatase natured antimicrobial mechanism. This novel class of carbon nanozymes has the potential to be used for effective catalytic and antibacterial applications.
AB - In this work, Sulfur and Nitrogen co-doped carbon nanoparticles (SN-CNPs) were synthesized by hydrothermal method using dried beet powder as the carbon source. TEM and AFM images indicated that these SN-CNPs form a round-shape ball with an approximate diameter of 50 nm. The presence of Sulfur and Nitrogen in these carbon-based nanoparticles was confirmed by FTIR and XPS analyses. These SN-CNPs were found to have strong phosphatase-like enzymatic activity. The enzymatic behavior of SN-CNPs follows the Michaelis-Menten mechanism with greater vmax and much lower Km values compared to alkaline phosphatase. Their antimicrobial properties were tested on E. coli and L. lactis, with MIC values of 63 μg mL-1 and 250 μg mL-1, respectively. SEM and AFM images of fixed and live E. coli cells revealed that SN-CNPs strongly interacted with the outer membranes of bacterial cells, significantly increasing the cell surface roughness. The chemical interaction between SN-CNPs and phospholipid modeled using quantum mechanical calculations further support our hypothesis that the phosphatase and antimicrobial properties of SN-CNPs are due to the thiol group on the SN-CNPs, which is a mimic of the cysteine-based protein phosphatase. The present work is the first to report carbon-based nanoparticles with strong phosphatase activity and propose a phosphatase natured antimicrobial mechanism. This novel class of carbon nanozymes has the potential to be used for effective catalytic and antibacterial applications.
KW - Carbon-based nanoparticles
KW - Enzymatic activity
KW - Minimum inhibitory concentration
KW - Phosphatase and antimicrobial properties
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85160015356&origin=inward&txGid=df9b4eb871af88e128550c44392acfd5
UR - https://www.mendeley.com/catalogue/305d34d5-0173-3983-aa14-91bd9bbe7556/
U2 - 10.1186/s11671-023-03856-y
DO - 10.1186/s11671-023-03856-y
M3 - Article
C2 - 37382706
VL - 18
JO - Discover nano
JF - Discover nano
SN - 2731-9229
IS - 1
M1 - 76
ER -
ID: 53342178