TY - JOUR

T1 - New Wolbachia pipientis Genotype Increasing Heat Stress Resistance of Drosophila melanogaster Host Is Characterized by a Large Chromosomal Inversion

AU - Korenskaia, Aleksandra E

AU - Shishkina, Olga D

AU - Klimenko, Alexandra I

AU - Andreenkova, Olga V

AU - Bobrovskikh, Margarita A

AU - Shatskaya, Natalja V

AU - Vasiliev, Gennady V

AU - Gruntenko, Nataly E

N1 - The study is supported by RSF grant No. 21-14-00090. The maintenance of experimental D. melanogaster strains was carried out in the Drosophila collection of the Institute of Cytology and Genetics SB RAS and was supported by BP #FWNR-2022-0019 of the Ministry of Science and Higher Education of the Russian Federation.

PY - 2022/12/19

Y1 - 2022/12/19

N2 - The maternally transmitted endocellular bacteria Wolbachia is a well-known symbiont of insects, demonstrating both negative and positive effects on host fitness. The previously found Wolbachia strain wMelPlus is characterized by a positive effect on the stress-resistance of its host Drosophila melanogaster, under heat stress conditions. This investigation is dedicated to studying the genomic underpinnings of such an effect. We sequenced two closely related Wolbachia strains, wMelPlus and wMelCS112, assembled their complete genomes, and performed comparative genomic analysis engaging available Wolbachia genomes from the wMel and wMelCS groups. Despite the two strains under study sharing very close gene-composition, we discovered a large (>1/6 of total genome) chromosomal inversion in wMelPlus, spanning through the region that includes the area of the inversion earlier found in the wMel group of Wolbachia genotypes. A number of genes in unique inversion blocks of wMelPlus were identified that might be involved in the induction of a stress-resistant phenotype in the host. We hypothesize that such an inversion could rearrange established genetic regulatory-networks, causing the observed effects of such a complex fly phenotype as a modulation of heat stress resistance. Based on our findings, we propose that wMelPlus be distinguished as a separate genotype of the wMelCS group, named wMelCS3.

AB - The maternally transmitted endocellular bacteria Wolbachia is a well-known symbiont of insects, demonstrating both negative and positive effects on host fitness. The previously found Wolbachia strain wMelPlus is characterized by a positive effect on the stress-resistance of its host Drosophila melanogaster, under heat stress conditions. This investigation is dedicated to studying the genomic underpinnings of such an effect. We sequenced two closely related Wolbachia strains, wMelPlus and wMelCS112, assembled their complete genomes, and performed comparative genomic analysis engaging available Wolbachia genomes from the wMel and wMelCS groups. Despite the two strains under study sharing very close gene-composition, we discovered a large (>1/6 of total genome) chromosomal inversion in wMelPlus, spanning through the region that includes the area of the inversion earlier found in the wMel group of Wolbachia genotypes. A number of genes in unique inversion blocks of wMelPlus were identified that might be involved in the induction of a stress-resistant phenotype in the host. We hypothesize that such an inversion could rearrange established genetic regulatory-networks, causing the observed effects of such a complex fly phenotype as a modulation of heat stress resistance. Based on our findings, we propose that wMelPlus be distinguished as a separate genotype of the wMelCS group, named wMelCS3.

KW - Animals

KW - Drosophila melanogaster/genetics

KW - Wolbachia/genetics

KW - Chromosome Inversion

KW - Genotype

KW - Heat-Shock Response/genetics

KW - Symbiosis

KW - wMelPlus

KW - genome assembly

KW - genome comparative analysis

KW - wMelCS

KW - chromosomal inversion

KW - Wolbachia

KW - Drosophila melanogaster

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85144558949&origin=inward&txGid=318153c786a4f898b7e01514e6b485d7

UR - https://www.mendeley.com/catalogue/f08b3691-9558-30d7-9a4e-a181511c5e31/

U2 - 10.3390/ijms232416212

DO - 10.3390/ijms232416212

M3 - Article

C2 - 36555851

VL - 23

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 24

M1 - 16212

ER -