Research output: Contribution to journal › Article › peer-review
New Wolbachia pipientis Genotype Increasing Heat Stress Resistance of Drosophila melanogaster Host Is Characterized by a Large Chromosomal Inversion. / Korenskaia, Aleksandra E; Shishkina, Olga D; Klimenko, Alexandra I et al.
In: International Journal of Molecular Sciences, Vol. 23, No. 24, 16212, 19.12.2022.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - New Wolbachia pipientis Genotype Increasing Heat Stress Resistance of Drosophila melanogaster Host Is Characterized by a Large Chromosomal Inversion
AU - Korenskaia, Aleksandra E
AU - Shishkina, Olga D
AU - Klimenko, Alexandra I
AU - Andreenkova, Olga V
AU - Bobrovskikh, Margarita A
AU - Shatskaya, Natalja V
AU - Vasiliev, Gennady V
AU - Gruntenko, Nataly E
N1 - The study is supported by RSF grant No. 21-14-00090. The maintenance of experimental D. melanogaster strains was carried out in the Drosophila collection of the Institute of Cytology and Genetics SB RAS and was supported by BP #FWNR-2022-0019 of the Ministry of Science and Higher Education of the Russian Federation.
PY - 2022/12/19
Y1 - 2022/12/19
N2 - The maternally transmitted endocellular bacteria Wolbachia is a well-known symbiont of insects, demonstrating both negative and positive effects on host fitness. The previously found Wolbachia strain wMelPlus is characterized by a positive effect on the stress-resistance of its host Drosophila melanogaster, under heat stress conditions. This investigation is dedicated to studying the genomic underpinnings of such an effect. We sequenced two closely related Wolbachia strains, wMelPlus and wMelCS112, assembled their complete genomes, and performed comparative genomic analysis engaging available Wolbachia genomes from the wMel and wMelCS groups. Despite the two strains under study sharing very close gene-composition, we discovered a large (>1/6 of total genome) chromosomal inversion in wMelPlus, spanning through the region that includes the area of the inversion earlier found in the wMel group of Wolbachia genotypes. A number of genes in unique inversion blocks of wMelPlus were identified that might be involved in the induction of a stress-resistant phenotype in the host. We hypothesize that such an inversion could rearrange established genetic regulatory-networks, causing the observed effects of such a complex fly phenotype as a modulation of heat stress resistance. Based on our findings, we propose that wMelPlus be distinguished as a separate genotype of the wMelCS group, named wMelCS3.
AB - The maternally transmitted endocellular bacteria Wolbachia is a well-known symbiont of insects, demonstrating both negative and positive effects on host fitness. The previously found Wolbachia strain wMelPlus is characterized by a positive effect on the stress-resistance of its host Drosophila melanogaster, under heat stress conditions. This investigation is dedicated to studying the genomic underpinnings of such an effect. We sequenced two closely related Wolbachia strains, wMelPlus and wMelCS112, assembled their complete genomes, and performed comparative genomic analysis engaging available Wolbachia genomes from the wMel and wMelCS groups. Despite the two strains under study sharing very close gene-composition, we discovered a large (>1/6 of total genome) chromosomal inversion in wMelPlus, spanning through the region that includes the area of the inversion earlier found in the wMel group of Wolbachia genotypes. A number of genes in unique inversion blocks of wMelPlus were identified that might be involved in the induction of a stress-resistant phenotype in the host. We hypothesize that such an inversion could rearrange established genetic regulatory-networks, causing the observed effects of such a complex fly phenotype as a modulation of heat stress resistance. Based on our findings, we propose that wMelPlus be distinguished as a separate genotype of the wMelCS group, named wMelCS3.
KW - Animals
KW - Drosophila melanogaster/genetics
KW - Wolbachia/genetics
KW - Chromosome Inversion
KW - Genotype
KW - Heat-Shock Response/genetics
KW - Symbiosis
KW - wMelPlus
KW - genome assembly
KW - genome comparative analysis
KW - wMelCS
KW - chromosomal inversion
KW - Wolbachia
KW - Drosophila melanogaster
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85144558949&origin=inward&txGid=318153c786a4f898b7e01514e6b485d7
UR - https://www.mendeley.com/catalogue/f08b3691-9558-30d7-9a4e-a181511c5e31/
U2 - 10.3390/ijms232416212
DO - 10.3390/ijms232416212
M3 - Article
C2 - 36555851
VL - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 24
M1 - 16212
ER -
ID: 42571806