TY - JOUR

T1 - New genetic lineage within the Siberian subtype of tick-borne encephalitis virus found in Western Siberia, Russia

AU - Tkachev, Sergey E.

AU - Chicherina, Galina S.

AU - Golovljova, Irina

AU - Belokopytova, Polina S.

AU - Tikunov, Artem Yu

AU - Zadora, Oksana V.

AU - Glupov, Victor V.

AU - Tikunova, Nina V.

N1 - Funding Information: This study was supported by the Russian Scientific Foundation (project No. 15-14-20020 ). Publisher Copyright: © 2017 Elsevier B.V. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 2017/12

Y1 - 2017/12

N2 - Tick-borne encephalitis virus (TBEV), a member of the Flaviviridae family, is a causative agent of a severe neurological disease. There are three main TBEV subtypes: the European (TBEV-Eu), Far Eastern (TBEV-FE), and Siberian (TBEV-Sib). Currently, three lineages within TBEV-Sib have been recorded. In this study, the genetic and biological characteristics of a new original strain, TBEV-2871, isolated in the Novosibirsk province of Western Siberia, Russia were investigated. The strain has low neuroinvasiveness in mice. Phylogenetic analysis demonstrated that TBEV-2871 belongs to TBEV-Sib, but does not cluster with any of the TBEV-Sib lineages. The TBEV-2871 strain has 88–89% nucleotide sequence identity with the other TBEV-Sib strains, 84–86% nucleotide sequence identity with the TBEV-FE and TBEV-Eu subtypes and is genetically close to the subtype division border. The TBEV-2871 polyprotein sequence includes 43 unique amino acid substitutions, 30 of which are recorded at positions that are conserved among all TBEV subtypes. Strain TBEV-2871 and two similar but not identical isolates found in Kemerovo province, Western Siberia are separated into a new lineage tentatively named Obskaya after the name of Ob riber, in the vicinity of which the TBEV-2871 was first found. A molecular evolution investigation demonstrated that within TBEV-Sib, the Obskaya lineage likely separated 1535 years ago, which is even earlier than the Baltic lineage.

AB - Tick-borne encephalitis virus (TBEV), a member of the Flaviviridae family, is a causative agent of a severe neurological disease. There are three main TBEV subtypes: the European (TBEV-Eu), Far Eastern (TBEV-FE), and Siberian (TBEV-Sib). Currently, three lineages within TBEV-Sib have been recorded. In this study, the genetic and biological characteristics of a new original strain, TBEV-2871, isolated in the Novosibirsk province of Western Siberia, Russia were investigated. The strain has low neuroinvasiveness in mice. Phylogenetic analysis demonstrated that TBEV-2871 belongs to TBEV-Sib, but does not cluster with any of the TBEV-Sib lineages. The TBEV-2871 strain has 88–89% nucleotide sequence identity with the other TBEV-Sib strains, 84–86% nucleotide sequence identity with the TBEV-FE and TBEV-Eu subtypes and is genetically close to the subtype division border. The TBEV-2871 polyprotein sequence includes 43 unique amino acid substitutions, 30 of which are recorded at positions that are conserved among all TBEV subtypes. Strain TBEV-2871 and two similar but not identical isolates found in Kemerovo province, Western Siberia are separated into a new lineage tentatively named Obskaya after the name of Ob riber, in the vicinity of which the TBEV-2871 was first found. A molecular evolution investigation demonstrated that within TBEV-Sib, the Obskaya lineage likely separated 1535 years ago, which is even earlier than the Baltic lineage.

KW - Lineages

KW - Siberian subtype

KW - Tick-borne encephalitis virus

KW - Western Siberia

KW - Genome, Viral

KW - Open Reading Frames

KW - Phylogeny

KW - Sequence Analysis, DNA

KW - Animals

KW - Encephalitis Viruses, Tick-Borne/classification

KW - Mice

KW - Siberia/epidemiology

KW - Encephalitis, Tick-Borne/epidemiology

UR - http://www.scopus.com/inward/record.url?scp=85032380215&partnerID=8YFLogxK

U2 - 10.1016/j.meegid.2017.10.020

DO - 10.1016/j.meegid.2017.10.020

M3 - Article

C2 - 29069610

AN - SCOPUS:85032380215

VL - 56

SP - 36

EP - 43

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

SN - 1567-1348

ER -