New genetic lineage within the Siberian subtype of tick-borne encephalitis virus found in Western Siberia, Russia. / Tkachev, Sergey E.; Chicherina, Galina S.; Golovljova, Irina et al.
In: Infection, Genetics and Evolution, Vol. 56, 12.2017, p. 36-43.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - New genetic lineage within the Siberian subtype of tick-borne encephalitis virus found in Western Siberia, Russia
AU - Tkachev, Sergey E.
AU - Chicherina, Galina S.
AU - Golovljova, Irina
AU - Belokopytova, Polina S.
AU - Tikunov, Artem Yu
AU - Zadora, Oksana V.
AU - Glupov, Victor V.
AU - Tikunova, Nina V.
N1 - Funding Information: This study was supported by the Russian Scientific Foundation (project No. 15-14-20020 ). Publisher Copyright: © 2017 Elsevier B.V. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2017/12
Y1 - 2017/12
N2 - Tick-borne encephalitis virus (TBEV), a member of the Flaviviridae family, is a causative agent of a severe neurological disease. There are three main TBEV subtypes: the European (TBEV-Eu), Far Eastern (TBEV-FE), and Siberian (TBEV-Sib). Currently, three lineages within TBEV-Sib have been recorded. In this study, the genetic and biological characteristics of a new original strain, TBEV-2871, isolated in the Novosibirsk province of Western Siberia, Russia were investigated. The strain has low neuroinvasiveness in mice. Phylogenetic analysis demonstrated that TBEV-2871 belongs to TBEV-Sib, but does not cluster with any of the TBEV-Sib lineages. The TBEV-2871 strain has 88–89% nucleotide sequence identity with the other TBEV-Sib strains, 84–86% nucleotide sequence identity with the TBEV-FE and TBEV-Eu subtypes and is genetically close to the subtype division border. The TBEV-2871 polyprotein sequence includes 43 unique amino acid substitutions, 30 of which are recorded at positions that are conserved among all TBEV subtypes. Strain TBEV-2871 and two similar but not identical isolates found in Kemerovo province, Western Siberia are separated into a new lineage tentatively named Obskaya after the name of Ob riber, in the vicinity of which the TBEV-2871 was first found. A molecular evolution investigation demonstrated that within TBEV-Sib, the Obskaya lineage likely separated 1535 years ago, which is even earlier than the Baltic lineage.
AB - Tick-borne encephalitis virus (TBEV), a member of the Flaviviridae family, is a causative agent of a severe neurological disease. There are three main TBEV subtypes: the European (TBEV-Eu), Far Eastern (TBEV-FE), and Siberian (TBEV-Sib). Currently, three lineages within TBEV-Sib have been recorded. In this study, the genetic and biological characteristics of a new original strain, TBEV-2871, isolated in the Novosibirsk province of Western Siberia, Russia were investigated. The strain has low neuroinvasiveness in mice. Phylogenetic analysis demonstrated that TBEV-2871 belongs to TBEV-Sib, but does not cluster with any of the TBEV-Sib lineages. The TBEV-2871 strain has 88–89% nucleotide sequence identity with the other TBEV-Sib strains, 84–86% nucleotide sequence identity with the TBEV-FE and TBEV-Eu subtypes and is genetically close to the subtype division border. The TBEV-2871 polyprotein sequence includes 43 unique amino acid substitutions, 30 of which are recorded at positions that are conserved among all TBEV subtypes. Strain TBEV-2871 and two similar but not identical isolates found in Kemerovo province, Western Siberia are separated into a new lineage tentatively named Obskaya after the name of Ob riber, in the vicinity of which the TBEV-2871 was first found. A molecular evolution investigation demonstrated that within TBEV-Sib, the Obskaya lineage likely separated 1535 years ago, which is even earlier than the Baltic lineage.
KW - Lineages
KW - Siberian subtype
KW - Tick-borne encephalitis virus
KW - Western Siberia
KW - Genome, Viral
KW - Open Reading Frames
KW - Phylogeny
KW - Sequence Analysis, DNA
KW - Animals
KW - Encephalitis Viruses, Tick-Borne/classification
KW - Mice
KW - Siberia/epidemiology
KW - Encephalitis, Tick-Borne/epidemiology
UR - http://www.scopus.com/inward/record.url?scp=85032380215&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2017.10.020
DO - 10.1016/j.meegid.2017.10.020
M3 - Article
C2 - 29069610
AN - SCOPUS:85032380215
VL - 56
SP - 36
EP - 43
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
SN - 1567-1348
ER -
ID: 27709743