TY - JOUR

T1 - Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune

AU - Timofeeva, Anna M.

AU - Sedykh, Sergey E.

AU - Ermakov, Evgeny A.

AU - Matveev, Andrey L.

AU - Odegova, Eva I.

AU - Sedykh, Tatiana A.

AU - Shcherbakov, Dmitry N.

AU - Merkuleva, Iuliia A.

AU - Volosnikova, Ekaterina A.

AU - Nesmeyanova, Valentina S.

AU - Tikunova, Nina V.

AU - Nevinsky, Georgy A.

N1 - Funding Information: This research was maintained by the Russian Science Foundation (Project 21-75-10105 to Anna Timofeeva—All the research except of statistical analysis) and the Russian State-funded budget project of ICBFM SB RAS 0245-2021-0009 (121031300041-4 to Georgy Nevinsky—Statistical analysis). Publisher Copyright: © 2022 by the authors.

PY - 2022/11

Y1 - 2022/11

N2 - Since the onset of the COVID-19 pandemic, numerous publications have appeared describing autoimmune pathologies developing after a coronavirus infection, with several papers reporting autoantibody production during the acute period of the disease. Several viral diseases are known to trigger autoimmune processes, and the appearance of catalytic antibodies with DNase activity is one of the earliest markers of several autoimmune pathologies. Therefore, we analyzed whether IgG antibodies from blood plasma of SARS-CoV-2 patients after recovery could bind and hydrolyze DNA. We analyzed how vaccination of patients with adenovirus Sputnik V vaccine influences the production of abzymes with DNase activity. Four groups were selected for the analysis, each containing 25 patients according to their relative titers of antibodies to S-protein: with high and median titers, vaccinated with Sputnik V with high titers, and a control group of donors with negative titers. The relative titers of antibodies against DNA and the relative DNase activity of IgGs depended very much on the individual patient and the donor, and no significant correlation was found between the relative values of antibodies titers and their DNase activity. Our results indicate that COVID-19 disease and vaccination with adenoviral Sputnik V vaccine do not result in the development or enhancement of strong autoimmune reactions as in the typical autoimmune diseases associated with the production of anti-DNA and DNA hydrolyzing antibodies.

AB - Since the onset of the COVID-19 pandemic, numerous publications have appeared describing autoimmune pathologies developing after a coronavirus infection, with several papers reporting autoantibody production during the acute period of the disease. Several viral diseases are known to trigger autoimmune processes, and the appearance of catalytic antibodies with DNase activity is one of the earliest markers of several autoimmune pathologies. Therefore, we analyzed whether IgG antibodies from blood plasma of SARS-CoV-2 patients after recovery could bind and hydrolyze DNA. We analyzed how vaccination of patients with adenovirus Sputnik V vaccine influences the production of abzymes with DNase activity. Four groups were selected for the analysis, each containing 25 patients according to their relative titers of antibodies to S-protein: with high and median titers, vaccinated with Sputnik V with high titers, and a control group of donors with negative titers. The relative titers of antibodies against DNA and the relative DNase activity of IgGs depended very much on the individual patient and the donor, and no significant correlation was found between the relative values of antibodies titers and their DNase activity. Our results indicate that COVID-19 disease and vaccination with adenoviral Sputnik V vaccine do not result in the development or enhancement of strong autoimmune reactions as in the typical autoimmune diseases associated with the production of anti-DNA and DNA hydrolyzing antibodies.

KW - anti-dsDNA-IgGs

KW - autoimmunity

KW - coronavirus

KW - COVID-19

KW - DNA

KW - RBD

KW - SARS-CoV-2

KW - spike protein

KW - Pandemics

KW - Antibodies, Antinuclear

KW - Humans

KW - Immunoglobulin G

KW - Deoxyribonucleases

KW - Viral Vaccines

KW - Antibodies, Catalytic

UR - http://www.scopus.com/inward/record.url?scp=85142851951&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/1a0d8880-2e1a-3915-aba2-039fff63d58a/

U2 - 10.3390/ijms232213681

DO - 10.3390/ijms232213681

M3 - Article

C2 - 36430159

AN - SCOPUS:85142851951

VL - 23

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 22

M1 - 13681

ER -