Research output: Contribution to journal › Article › peer-review
Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune. / Timofeeva, Anna M.; Sedykh, Sergey E.; Ermakov, Evgeny A. et al.
In: International Journal of Molecular Sciences, Vol. 23, No. 22, 13681, 11.2022.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune
AU - Timofeeva, Anna M.
AU - Sedykh, Sergey E.
AU - Ermakov, Evgeny A.
AU - Matveev, Andrey L.
AU - Odegova, Eva I.
AU - Sedykh, Tatiana A.
AU - Shcherbakov, Dmitry N.
AU - Merkuleva, Iuliia A.
AU - Volosnikova, Ekaterina A.
AU - Nesmeyanova, Valentina S.
AU - Tikunova, Nina V.
AU - Nevinsky, Georgy A.
N1 - Funding Information: This research was maintained by the Russian Science Foundation (Project 21-75-10105 to Anna Timofeeva—All the research except of statistical analysis) and the Russian State-funded budget project of ICBFM SB RAS 0245-2021-0009 (121031300041-4 to Georgy Nevinsky—Statistical analysis). Publisher Copyright: © 2022 by the authors.
PY - 2022/11
Y1 - 2022/11
N2 - Since the onset of the COVID-19 pandemic, numerous publications have appeared describing autoimmune pathologies developing after a coronavirus infection, with several papers reporting autoantibody production during the acute period of the disease. Several viral diseases are known to trigger autoimmune processes, and the appearance of catalytic antibodies with DNase activity is one of the earliest markers of several autoimmune pathologies. Therefore, we analyzed whether IgG antibodies from blood plasma of SARS-CoV-2 patients after recovery could bind and hydrolyze DNA. We analyzed how vaccination of patients with adenovirus Sputnik V vaccine influences the production of abzymes with DNase activity. Four groups were selected for the analysis, each containing 25 patients according to their relative titers of antibodies to S-protein: with high and median titers, vaccinated with Sputnik V with high titers, and a control group of donors with negative titers. The relative titers of antibodies against DNA and the relative DNase activity of IgGs depended very much on the individual patient and the donor, and no significant correlation was found between the relative values of antibodies titers and their DNase activity. Our results indicate that COVID-19 disease and vaccination with adenoviral Sputnik V vaccine do not result in the development or enhancement of strong autoimmune reactions as in the typical autoimmune diseases associated with the production of anti-DNA and DNA hydrolyzing antibodies.
AB - Since the onset of the COVID-19 pandemic, numerous publications have appeared describing autoimmune pathologies developing after a coronavirus infection, with several papers reporting autoantibody production during the acute period of the disease. Several viral diseases are known to trigger autoimmune processes, and the appearance of catalytic antibodies with DNase activity is one of the earliest markers of several autoimmune pathologies. Therefore, we analyzed whether IgG antibodies from blood plasma of SARS-CoV-2 patients after recovery could bind and hydrolyze DNA. We analyzed how vaccination of patients with adenovirus Sputnik V vaccine influences the production of abzymes with DNase activity. Four groups were selected for the analysis, each containing 25 patients according to their relative titers of antibodies to S-protein: with high and median titers, vaccinated with Sputnik V with high titers, and a control group of donors with negative titers. The relative titers of antibodies against DNA and the relative DNase activity of IgGs depended very much on the individual patient and the donor, and no significant correlation was found between the relative values of antibodies titers and their DNase activity. Our results indicate that COVID-19 disease and vaccination with adenoviral Sputnik V vaccine do not result in the development or enhancement of strong autoimmune reactions as in the typical autoimmune diseases associated with the production of anti-DNA and DNA hydrolyzing antibodies.
KW - anti-dsDNA-IgGs
KW - autoimmunity
KW - coronavirus
KW - COVID-19
KW - DNA
KW - RBD
KW - SARS-CoV-2
KW - spike protein
KW - Pandemics
KW - Antibodies, Antinuclear
KW - Humans
KW - Immunoglobulin G
KW - Deoxyribonucleases
KW - Viral Vaccines
KW - Antibodies, Catalytic
UR - http://www.scopus.com/inward/record.url?scp=85142851951&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/1a0d8880-2e1a-3915-aba2-039fff63d58a/
U2 - 10.3390/ijms232213681
DO - 10.3390/ijms232213681
M3 - Article
C2 - 36430159
AN - SCOPUS:85142851951
VL - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 22
M1 - 13681
ER -
ID: 40098173