TY - JOUR

T1 - Mitochondrial DNA analysis of ancient sheep from Altai

AU - Dymova, M. A.

AU - Zadorozhny, A. V.

AU - Mishukova, O. V.

AU - Khrapov, E. A.

AU - Druzhkova, A. S.

AU - Trifonov, V. A.

AU - Kichigin, I. G.

AU - Tishkin, A. A.

AU - Grushin, S. P.

AU - Filipenko, M. L.

N1 - Publisher Copyright: © 2017 Stichting International Foundation for Animal Genetics

PY - 2017/10/1

Y1 - 2017/10/1

N2 - A comparative analysis of the genetic diversity of ancient and modern sheep can shed light on the origin of these animals and their distribution as well as help to evaluate the role of humans at each formation stage of different sheep breeds. Here we isolated ancient DNA and performed sequencing of the mitochondrial DNA D-loop from 17 sheep bone remains (~4000–1000 years old) found in the archaeological complexes in the south of Altai (Western Siberia). The length of the sequences obtained ranged between 318 and 586 bp. The haplotype diversity and nucleotide diversity were 0.801 ± 0.081 and 0.0096 ± 0.0014 respectively. The average number of nucleotide differences was ~3.1. Nucleotide sequence analysis revealed that 15 specimens were nested within previously described A,B,C,D and E lineages and that two specimens had a basal position relative to the rest of the analyzed samples. A relatively high diversity of sheep haplotypes, including the presence of two basal haplotypes, indicates that the Altai region may have been a transport route of human migration. Further ancient DNA analysis of other specimens and deeper genome sequencing of samples with novel haplotypes is needed to better understand the demographic history of sheep in Southern Siberia.

AB - A comparative analysis of the genetic diversity of ancient and modern sheep can shed light on the origin of these animals and their distribution as well as help to evaluate the role of humans at each formation stage of different sheep breeds. Here we isolated ancient DNA and performed sequencing of the mitochondrial DNA D-loop from 17 sheep bone remains (~4000–1000 years old) found in the archaeological complexes in the south of Altai (Western Siberia). The length of the sequences obtained ranged between 318 and 586 bp. The haplotype diversity and nucleotide diversity were 0.801 ± 0.081 and 0.0096 ± 0.0014 respectively. The average number of nucleotide differences was ~3.1. Nucleotide sequence analysis revealed that 15 specimens were nested within previously described A,B,C,D and E lineages and that two specimens had a basal position relative to the rest of the analyzed samples. A relatively high diversity of sheep haplotypes, including the presence of two basal haplotypes, indicates that the Altai region may have been a transport route of human migration. Further ancient DNA analysis of other specimens and deeper genome sequencing of samples with novel haplotypes is needed to better understand the demographic history of sheep in Southern Siberia.

KW - aDNA

KW - archaeological remains

KW - domestication

KW - haplotyping

KW - lineage

KW - Ovis aries

KW - sequencing

KW - Western Siberia

KW - Haplotypes

KW - Sheep/genetics

KW - Sequence Analysis, DNA/veterinary

KW - Breeding

KW - DNA, Ancient/analysis

KW - DNA, Mitochondrial/genetics

KW - Phylogeny

KW - Genetic Variation

KW - Archaeology

KW - Animals

KW - Siberia

KW - Evolution, Molecular

UR - http://www.scopus.com/inward/record.url?scp=85020004385&partnerID=8YFLogxK

U2 - 10.1111/age.12569

DO - 10.1111/age.12569

M3 - Article

C2 - 28568904

AN - SCOPUS:85020004385

VL - 48

SP - 615

EP - 618

JO - BMC Genetics

JF - BMC Genetics

SN - 1471-2156

IS - 5

ER -