Research output: Contribution to journal › Article › peer-review
Mitochondrial DNA analysis of ancient sheep from Altai. / Dymova, M. A.; Zadorozhny, A. V.; Mishukova, O. V. et al.
In: BMC Genetics, Vol. 48, No. 5, 01.10.2017, p. 615-618.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Mitochondrial DNA analysis of ancient sheep from Altai
AU - Dymova, M. A.
AU - Zadorozhny, A. V.
AU - Mishukova, O. V.
AU - Khrapov, E. A.
AU - Druzhkova, A. S.
AU - Trifonov, V. A.
AU - Kichigin, I. G.
AU - Tishkin, A. A.
AU - Grushin, S. P.
AU - Filipenko, M. L.
N1 - Publisher Copyright: © 2017 Stichting International Foundation for Animal Genetics
PY - 2017/10/1
Y1 - 2017/10/1
N2 - A comparative analysis of the genetic diversity of ancient and modern sheep can shed light on the origin of these animals and their distribution as well as help to evaluate the role of humans at each formation stage of different sheep breeds. Here we isolated ancient DNA and performed sequencing of the mitochondrial DNA D-loop from 17 sheep bone remains (~4000–1000 years old) found in the archaeological complexes in the south of Altai (Western Siberia). The length of the sequences obtained ranged between 318 and 586 bp. The haplotype diversity and nucleotide diversity were 0.801 ± 0.081 and 0.0096 ± 0.0014 respectively. The average number of nucleotide differences was ~3.1. Nucleotide sequence analysis revealed that 15 specimens were nested within previously described A,B,C,D and E lineages and that two specimens had a basal position relative to the rest of the analyzed samples. A relatively high diversity of sheep haplotypes, including the presence of two basal haplotypes, indicates that the Altai region may have been a transport route of human migration. Further ancient DNA analysis of other specimens and deeper genome sequencing of samples with novel haplotypes is needed to better understand the demographic history of sheep in Southern Siberia.
AB - A comparative analysis of the genetic diversity of ancient and modern sheep can shed light on the origin of these animals and their distribution as well as help to evaluate the role of humans at each formation stage of different sheep breeds. Here we isolated ancient DNA and performed sequencing of the mitochondrial DNA D-loop from 17 sheep bone remains (~4000–1000 years old) found in the archaeological complexes in the south of Altai (Western Siberia). The length of the sequences obtained ranged between 318 and 586 bp. The haplotype diversity and nucleotide diversity were 0.801 ± 0.081 and 0.0096 ± 0.0014 respectively. The average number of nucleotide differences was ~3.1. Nucleotide sequence analysis revealed that 15 specimens were nested within previously described A,B,C,D and E lineages and that two specimens had a basal position relative to the rest of the analyzed samples. A relatively high diversity of sheep haplotypes, including the presence of two basal haplotypes, indicates that the Altai region may have been a transport route of human migration. Further ancient DNA analysis of other specimens and deeper genome sequencing of samples with novel haplotypes is needed to better understand the demographic history of sheep in Southern Siberia.
KW - aDNA
KW - archaeological remains
KW - domestication
KW - haplotyping
KW - lineage
KW - Ovis aries
KW - sequencing
KW - Western Siberia
KW - Haplotypes
KW - Sheep/genetics
KW - Sequence Analysis, DNA/veterinary
KW - Breeding
KW - DNA, Ancient/analysis
KW - DNA, Mitochondrial/genetics
KW - Phylogeny
KW - Genetic Variation
KW - Archaeology
KW - Animals
KW - Siberia
KW - Evolution, Molecular
UR - http://www.scopus.com/inward/record.url?scp=85020004385&partnerID=8YFLogxK
U2 - 10.1111/age.12569
DO - 10.1111/age.12569
M3 - Article
C2 - 28568904
AN - SCOPUS:85020004385
VL - 48
SP - 615
EP - 618
JO - BMC Genetics
JF - BMC Genetics
SN - 1471-2156
IS - 5
ER -
ID: 9069809