TY - JOUR

T1 - Methods for Molecular Evolution of Polymerases

AU - Zhukov, S. A.

AU - Fokina, A. A.

AU - Stetsenko, D. A.

AU - Vasilyeva, S. V.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - In 2018, three scientists shared the Nobel Prize in Chemistry: Frances H. Arnold, “for the directed evolution of enzymes”; George P. Smith and Sir Gregory P. Winter, “for the phage display of peptides and antibodies”. All authors are associated with the development of methods for obtaining the useful proteins and peptides, which are based on imitation of the natural “method” of biological evolution, namely, on a combination of random variability and nonrandom selection. In this review, we consider modern methods for designing engineering polymerases, which determine the progress in the enzymatic synthesis and evolution of unnatural nucleic acids. The development of these methods provides an opportunity to obtain and study a huge set of new biopolymers inaccessible to nature, as well as various ligands, catalysts, and materials based thereon.

AB - In 2018, three scientists shared the Nobel Prize in Chemistry: Frances H. Arnold, “for the directed evolution of enzymes”; George P. Smith and Sir Gregory P. Winter, “for the phage display of peptides and antibodies”. All authors are associated with the development of methods for obtaining the useful proteins and peptides, which are based on imitation of the natural “method” of biological evolution, namely, on a combination of random variability and nonrandom selection. In this review, we consider modern methods for designing engineering polymerases, which determine the progress in the enzymatic synthesis and evolution of unnatural nucleic acids. The development of these methods provides an opportunity to obtain and study a huge set of new biopolymers inaccessible to nature, as well as various ligands, catalysts, and materials based thereon.

KW - compartmentalized self-replication

KW - directed evolution of polymerases

KW - new enzymes

KW - phage display

KW - rational design

KW - screening

UR - http://www.scopus.com/inward/record.url?scp=85077852572&partnerID=8YFLogxK

U2 - 10.1134/S1068162019060426

DO - 10.1134/S1068162019060426

M3 - Article

AN - SCOPUS:85077852572

VL - 45

SP - 726

EP - 738

JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

SN - 1068-1620

IS - 6

ER -