Research output: Contribution to journal › Article › peer-review
Methods for Molecular Evolution of Polymerases. / Zhukov, S. A.; Fokina, A. A.; Stetsenko, D. A. et al.
In: Russian Journal of Bioorganic Chemistry, Vol. 45, No. 6, 01.11.2019, p. 726-738.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Methods for Molecular Evolution of Polymerases
AU - Zhukov, S. A.
AU - Fokina, A. A.
AU - Stetsenko, D. A.
AU - Vasilyeva, S. V.
PY - 2019/11/1
Y1 - 2019/11/1
N2 - In 2018, three scientists shared the Nobel Prize in Chemistry: Frances H. Arnold, “for the directed evolution of enzymes”; George P. Smith and Sir Gregory P. Winter, “for the phage display of peptides and antibodies”. All authors are associated with the development of methods for obtaining the useful proteins and peptides, which are based on imitation of the natural “method” of biological evolution, namely, on a combination of random variability and nonrandom selection. In this review, we consider modern methods for designing engineering polymerases, which determine the progress in the enzymatic synthesis and evolution of unnatural nucleic acids. The development of these methods provides an opportunity to obtain and study a huge set of new biopolymers inaccessible to nature, as well as various ligands, catalysts, and materials based thereon.
AB - In 2018, three scientists shared the Nobel Prize in Chemistry: Frances H. Arnold, “for the directed evolution of enzymes”; George P. Smith and Sir Gregory P. Winter, “for the phage display of peptides and antibodies”. All authors are associated with the development of methods for obtaining the useful proteins and peptides, which are based on imitation of the natural “method” of biological evolution, namely, on a combination of random variability and nonrandom selection. In this review, we consider modern methods for designing engineering polymerases, which determine the progress in the enzymatic synthesis and evolution of unnatural nucleic acids. The development of these methods provides an opportunity to obtain and study a huge set of new biopolymers inaccessible to nature, as well as various ligands, catalysts, and materials based thereon.
KW - compartmentalized self-replication
KW - directed evolution of polymerases
KW - new enzymes
KW - phage display
KW - rational design
KW - screening
UR - http://www.scopus.com/inward/record.url?scp=85077852572&partnerID=8YFLogxK
U2 - 10.1134/S1068162019060426
DO - 10.1134/S1068162019060426
M3 - Article
AN - SCOPUS:85077852572
VL - 45
SP - 726
EP - 738
JO - Russian Journal of Bioorganic Chemistry
JF - Russian Journal of Bioorganic Chemistry
SN - 1068-1620
IS - 6
ER -
ID: 23709322