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Mechanisms of the Specificity of the CRISPR/Cas9 System in Genome Editing. / Kulishova, L. M.; Vokhtantsev, I. P.; Kim, D. V. и др.

в: Molecular Biology, Том 57, № 2, 04.2023, стр. 258-271.

Результаты исследований: Научные публикации в периодических изданияхстатьяРецензирование

Harvard

Kulishova, LM, Vokhtantsev, IP, Kim, DV & Zharkov, DO 2023, 'Mechanisms of the Specificity of the CRISPR/Cas9 System in Genome Editing', Molecular Biology, Том. 57, № 2, стр. 258-271. https://doi.org/10.1134/S0026893323020139

APA

Vancouver

Kulishova LM, Vokhtantsev IP, Kim DV, Zharkov DO. Mechanisms of the Specificity of the CRISPR/Cas9 System in Genome Editing. Molecular Biology. 2023 апр.;57(2):258-271. doi: 10.1134/S0026893323020139

Author

Kulishova, L. M. ; Vokhtantsev, I. P. ; Kim, D. V. и др. / Mechanisms of the Specificity of the CRISPR/Cas9 System in Genome Editing. в: Molecular Biology. 2023 ; Том 57, № 2. стр. 258-271.

BibTeX

@article{b304095828d44527a307819934fff12f,
title = "Mechanisms of the Specificity of the CRISPR/Cas9 System in Genome Editing",
abstract = "The CRISPR/Cas9 system, which was discovered recently, utilizes nucleases targeted by sequence complementarity and is originally intended to protect bacteria from foreign genetic elements. The system provided a convenient tool for manipulating the genomes of living cells. The CRISPR/Cas9 genomic editing technology moved beyond the laboratory and already found application in biotechnology and agriculture. However, off-target activity of the CRISPR/Cas9 system can cause oncogenic mutations and thus limits its use for genome editing in human cells for medical purposes. Many studies are therefore aimed at developing variants of the CRISPR/Cas9 system with improved accuracy. The review considers the mechanisms of precise and erroneous actions of Cas9 RNA-guided nuclease, natural and artificial variants of RNA-targeted nucleases, possibilities to modulate their specificity through guide RNA modifications, and other approaches to increasing the accuracy of the CRISPR/Cas9 system in genome editing.",
keywords = "CRISPR/Cas9, Cas9 protein, enzyme specificity, genome editing, mutations, off-target effects, protein engineering",
author = "Kulishova, {L. M.} and Vokhtantsev, {I. P.} and Kim, {D. V.} and Zharkov, {D. O.}",
note = "This work was supported by the Russian Science Foundation (project no. 21-64-00017). D.V. Kim acknowledges support from the Russian Foundation for Basic Research (project no. 20-34-90092, analysis of the cell mechanisms of specificity”). D.O. Zharkov acknowledges support from the Ministry of Higher Education and Science of the Russian Federation (state contract no. 121031300056-8, structural analysis of enzyme specificity). Публикация для корректировки.",
year = "2023",
month = apr,
doi = "10.1134/S0026893323020139",
language = "English",
volume = "57",
pages = "258--271",
journal = "Molecular Biology",
issn = "0026-8933",
publisher = "Maik Nauka-Interperiodica Publishing",
number = "2",

}

RIS

TY - JOUR

T1 - Mechanisms of the Specificity of the CRISPR/Cas9 System in Genome Editing

AU - Kulishova, L. M.

AU - Vokhtantsev, I. P.

AU - Kim, D. V.

AU - Zharkov, D. O.

N1 - This work was supported by the Russian Science Foundation (project no. 21-64-00017). D.V. Kim acknowledges support from the Russian Foundation for Basic Research (project no. 20-34-90092, analysis of the cell mechanisms of specificity”). D.O. Zharkov acknowledges support from the Ministry of Higher Education and Science of the Russian Federation (state contract no. 121031300056-8, structural analysis of enzyme specificity). Публикация для корректировки.

PY - 2023/4

Y1 - 2023/4

N2 - The CRISPR/Cas9 system, which was discovered recently, utilizes nucleases targeted by sequence complementarity and is originally intended to protect bacteria from foreign genetic elements. The system provided a convenient tool for manipulating the genomes of living cells. The CRISPR/Cas9 genomic editing technology moved beyond the laboratory and already found application in biotechnology and agriculture. However, off-target activity of the CRISPR/Cas9 system can cause oncogenic mutations and thus limits its use for genome editing in human cells for medical purposes. Many studies are therefore aimed at developing variants of the CRISPR/Cas9 system with improved accuracy. The review considers the mechanisms of precise and erroneous actions of Cas9 RNA-guided nuclease, natural and artificial variants of RNA-targeted nucleases, possibilities to modulate their specificity through guide RNA modifications, and other approaches to increasing the accuracy of the CRISPR/Cas9 system in genome editing.

AB - The CRISPR/Cas9 system, which was discovered recently, utilizes nucleases targeted by sequence complementarity and is originally intended to protect bacteria from foreign genetic elements. The system provided a convenient tool for manipulating the genomes of living cells. The CRISPR/Cas9 genomic editing technology moved beyond the laboratory and already found application in biotechnology and agriculture. However, off-target activity of the CRISPR/Cas9 system can cause oncogenic mutations and thus limits its use for genome editing in human cells for medical purposes. Many studies are therefore aimed at developing variants of the CRISPR/Cas9 system with improved accuracy. The review considers the mechanisms of precise and erroneous actions of Cas9 RNA-guided nuclease, natural and artificial variants of RNA-targeted nucleases, possibilities to modulate their specificity through guide RNA modifications, and other approaches to increasing the accuracy of the CRISPR/Cas9 system in genome editing.

KW - CRISPR/Cas9

KW - Cas9 protein

KW - enzyme specificity

KW - genome editing

KW - mutations

KW - off-target effects

KW - protein engineering

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85156091694&origin=inward&txGid=2b9e7d66c5208f2d65fbd213fea323a7

UR - https://www.mendeley.com/catalogue/916b4f36-5f0a-3477-8a7a-aeb114efc26e/

U2 - 10.1134/S0026893323020139

DO - 10.1134/S0026893323020139

M3 - Article

VL - 57

SP - 258

EP - 271

JO - Molecular Biology

JF - Molecular Biology

SN - 0026-8933

IS - 2

ER -

ID: 59649476