Research output: Contribution to journal › Article › peer-review
Mechanisms of the Specificity of the CRISPR/Cas9 System in Genome Editing. / Kulishova, L. M.; Vokhtantsev, I. P.; Kim, D. V. et al.
In: Molecular Biology, Vol. 57, No. 2, 04.2023, p. 258-271.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Mechanisms of the Specificity of the CRISPR/Cas9 System in Genome Editing
AU - Kulishova, L. M.
AU - Vokhtantsev, I. P.
AU - Kim, D. V.
AU - Zharkov, D. O.
N1 - This work was supported by the Russian Science Foundation (project no. 21-64-00017). D.V. Kim acknowledges support from the Russian Foundation for Basic Research (project no. 20-34-90092, analysis of the cell mechanisms of specificity”). D.O. Zharkov acknowledges support from the Ministry of Higher Education and Science of the Russian Federation (state contract no. 121031300056-8, structural analysis of enzyme specificity). Публикация для корректировки.
PY - 2023/4
Y1 - 2023/4
N2 - The CRISPR/Cas9 system, which was discovered recently, utilizes nucleases targeted by sequence complementarity and is originally intended to protect bacteria from foreign genetic elements. The system provided a convenient tool for manipulating the genomes of living cells. The CRISPR/Cas9 genomic editing technology moved beyond the laboratory and already found application in biotechnology and agriculture. However, off-target activity of the CRISPR/Cas9 system can cause oncogenic mutations and thus limits its use for genome editing in human cells for medical purposes. Many studies are therefore aimed at developing variants of the CRISPR/Cas9 system with improved accuracy. The review considers the mechanisms of precise and erroneous actions of Cas9 RNA-guided nuclease, natural and artificial variants of RNA-targeted nucleases, possibilities to modulate their specificity through guide RNA modifications, and other approaches to increasing the accuracy of the CRISPR/Cas9 system in genome editing.
AB - The CRISPR/Cas9 system, which was discovered recently, utilizes nucleases targeted by sequence complementarity and is originally intended to protect bacteria from foreign genetic elements. The system provided a convenient tool for manipulating the genomes of living cells. The CRISPR/Cas9 genomic editing technology moved beyond the laboratory and already found application in biotechnology and agriculture. However, off-target activity of the CRISPR/Cas9 system can cause oncogenic mutations and thus limits its use for genome editing in human cells for medical purposes. Many studies are therefore aimed at developing variants of the CRISPR/Cas9 system with improved accuracy. The review considers the mechanisms of precise and erroneous actions of Cas9 RNA-guided nuclease, natural and artificial variants of RNA-targeted nucleases, possibilities to modulate their specificity through guide RNA modifications, and other approaches to increasing the accuracy of the CRISPR/Cas9 system in genome editing.
KW - CRISPR/Cas9
KW - Cas9 protein
KW - enzyme specificity
KW - genome editing
KW - mutations
KW - off-target effects
KW - protein engineering
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85156091694&origin=inward&txGid=2b9e7d66c5208f2d65fbd213fea323a7
UR - https://www.mendeley.com/catalogue/916b4f36-5f0a-3477-8a7a-aeb114efc26e/
U2 - 10.1134/S0026893323020139
DO - 10.1134/S0026893323020139
M3 - Article
VL - 57
SP - 258
EP - 271
JO - Molecular Biology
JF - Molecular Biology
SN - 0026-8933
IS - 2
ER -
ID: 59649476