Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Mechanical properties and biological behavior of 3D matrices produced by electrospinning from protein-enriched polyurethane. / Chernonosova, Vera S.; Gostev, Alexander A.; Gao, Yun и др.
в: BioMed Research International, Том 2018, 1380606, 01.01.2018, стр. 1380606.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Mechanical properties and biological behavior of 3D matrices produced by electrospinning from protein-enriched polyurethane
AU - Chernonosova, Vera S.
AU - Gostev, Alexander A.
AU - Gao, Yun
AU - Chesalov, Yuriy A.
AU - Shutov, Alexey V.
AU - Pokushalov, Evgeniy A.
AU - Karpenko, Andrey A.
AU - Laktionov, Pavel P.
N1 - Publisher Copyright: © 2018 Vera S. Chernonosova et al.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Properties of matrices manufactured by electrospinning from solutions of polyurethane Tecoflex EG-80A with gelatin in 1,1,1,3,3,3-hexafluoroisopropanol were studied. The concentration of gelatin added to the electrospinning solution was shown to influence the mechanical properties of matrices: the dependence of matrix tensile strength on protein concentration is described by a bell-shaped curve and an increase in gelatin concentration added to the elasticity of the samples. SEM, FTIR spectroscopy, and mechanical testing demonstrate that incubation of matrices in phosphate buffer changes the structure of the fibers and alters the polyurethane-gelatin interactions, increasing matrix durability. The ability of the matrices to maintain adhesion and proliferation of human endothelial cells was studied. The results suggest that matrices made of 3% polyurethane solution with 15% gelatin (wt/wt) and treated with glutaraldehyde are the optimal variant for cultivation of endothelial cells.
AB - Properties of matrices manufactured by electrospinning from solutions of polyurethane Tecoflex EG-80A with gelatin in 1,1,1,3,3,3-hexafluoroisopropanol were studied. The concentration of gelatin added to the electrospinning solution was shown to influence the mechanical properties of matrices: the dependence of matrix tensile strength on protein concentration is described by a bell-shaped curve and an increase in gelatin concentration added to the elasticity of the samples. SEM, FTIR spectroscopy, and mechanical testing demonstrate that incubation of matrices in phosphate buffer changes the structure of the fibers and alters the polyurethane-gelatin interactions, increasing matrix durability. The ability of the matrices to maintain adhesion and proliferation of human endothelial cells was studied. The results suggest that matrices made of 3% polyurethane solution with 15% gelatin (wt/wt) and treated with glutaraldehyde are the optimal variant for cultivation of endothelial cells.
KW - Elasticity
KW - Endothelial Cells
KW - Gelatin
KW - Humans
KW - Polyurethanes/chemistry
KW - Tensile Strength
KW - Tissue Engineering
KW - GELATIN
KW - TECOPHILIC/GELATIN NANOFIBERS
KW - VASCULAR GRAFT
KW - RELEASE
KW - SURFACE-ROUGHNESS
KW - HUMAN ENDOTHELIAL-CELLS
KW - IN-VITRO
KW - TISSUE ENGINEERING APPLICATIONS
KW - DEGRADATION
KW - SCAFFOLDS
UR - http://www.scopus.com/inward/record.url?scp=85049830964&partnerID=8YFLogxK
U2 - 10.1155/2018/1380606
DO - 10.1155/2018/1380606
M3 - Article
C2 - 30046587
AN - SCOPUS:85049830964
VL - 2018
SP - 1380606
JO - BioMed Research International
JF - BioMed Research International
SN - 2314-6133
M1 - 1380606
ER -
ID: 14883008