TY - JOUR

T1 - Identification of sex chromosomes in Eremias velox (Lacertidae, Reptilia) using lampbrush chromosome analysis

AU - Lisachov, Artem P.

AU - Galkina, Svetlana A.

AU - Saifitdinova, Alsu F.

AU - Romanenko, Svetlana A.

AU - Andreyushkova, Daria A.

AU - Trifonov, Vladimir A.

AU - Borodin, Pavel M.

N1 - Publisher Copyright: © Artem P. Lisachov et al.

PY - 2019/5/14

Y1 - 2019/5/14

N2 - Reptiles are good objects for studying the evolution of sex determination, since they have different sex determination systems in different lineages. Lacertid lizards have been long-known for possessing ZZ/ ZW type sex chromosomes. However, due to morphological uniformity of lacertid chromosomes, the Z chromosome has been only putatively cytologically identified. We used lampbrush chromosome (LBC) analysis and FISH with a W-specific probe in Eremias velox (Pallas, 1771) to unequivocally identify the ZW bivalent and investigate its meiotic behavior. The heterochromatic W chromosome is decondensed at the lampbrush stage, indicating active transcription, contrast with the highly condensed condition of the lampbrush W chromosomes in birds. We identified the Z chromosome by its chiasmatic association with the W chromosome as chromosome XIII of the 19 chromosomes in the LBC karyotype. Our findings agree with previous genetic and genomic studies, which suggested that the lacertid Z chromosome should be one of the smaller macrochromosomes.

AB - Reptiles are good objects for studying the evolution of sex determination, since they have different sex determination systems in different lineages. Lacertid lizards have been long-known for possessing ZZ/ ZW type sex chromosomes. However, due to morphological uniformity of lacertid chromosomes, the Z chromosome has been only putatively cytologically identified. We used lampbrush chromosome (LBC) analysis and FISH with a W-specific probe in Eremias velox (Pallas, 1771) to unequivocally identify the ZW bivalent and investigate its meiotic behavior. The heterochromatic W chromosome is decondensed at the lampbrush stage, indicating active transcription, contrast with the highly condensed condition of the lampbrush W chromosomes in birds. We identified the Z chromosome by its chiasmatic association with the W chromosome as chromosome XIII of the 19 chromosomes in the LBC karyotype. Our findings agree with previous genetic and genomic studies, which suggested that the lacertid Z chromosome should be one of the smaller macrochromosomes.

KW - Heterochromatin

KW - Lampbrush chromosomes

KW - Lizard

KW - Meiosis

KW - Microdissection

KW - Sex chromosomes

KW - RECOMBINATION

KW - GECKO

KW - meiosis

KW - sex chromosomes

KW - GENOME

KW - heterochromatin

KW - EVOLUTION

KW - lampbrush chromosomes

KW - DNA

KW - microdissection

KW - KARYOTYPE

KW - lizard

KW - INSIGHTS

UR - http://www.scopus.com/inward/record.url?scp=85067334464&partnerID=8YFLogxK

U2 - 10.3897/CompCytogen.v13i2.34116

DO - 10.3897/CompCytogen.v13i2.34116

M3 - Article

C2 - 31149328

AN - SCOPUS:85067334464

VL - 13

SP - 121

EP - 132

JO - Comparative Cytogenetics

JF - Comparative Cytogenetics

SN - 1993-0771

IS - 2

ER -