Research output: Contribution to journal › Article › peer-review
Identification of sex chromosomes in Eremias velox (Lacertidae, Reptilia) using lampbrush chromosome analysis. / Lisachov, Artem P.; Galkina, Svetlana A.; Saifitdinova, Alsu F. et al.
In: Comparative Cytogenetics, Vol. 13, No. 2, 14.05.2019, p. 121-132.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Identification of sex chromosomes in Eremias velox (Lacertidae, Reptilia) using lampbrush chromosome analysis
AU - Lisachov, Artem P.
AU - Galkina, Svetlana A.
AU - Saifitdinova, Alsu F.
AU - Romanenko, Svetlana A.
AU - Andreyushkova, Daria A.
AU - Trifonov, Vladimir A.
AU - Borodin, Pavel M.
N1 - Publisher Copyright: © Artem P. Lisachov et al.
PY - 2019/5/14
Y1 - 2019/5/14
N2 - Reptiles are good objects for studying the evolution of sex determination, since they have different sex determination systems in different lineages. Lacertid lizards have been long-known for possessing ZZ/ ZW type sex chromosomes. However, due to morphological uniformity of lacertid chromosomes, the Z chromosome has been only putatively cytologically identified. We used lampbrush chromosome (LBC) analysis and FISH with a W-specific probe in Eremias velox (Pallas, 1771) to unequivocally identify the ZW bivalent and investigate its meiotic behavior. The heterochromatic W chromosome is decondensed at the lampbrush stage, indicating active transcription, contrast with the highly condensed condition of the lampbrush W chromosomes in birds. We identified the Z chromosome by its chiasmatic association with the W chromosome as chromosome XIII of the 19 chromosomes in the LBC karyotype. Our findings agree with previous genetic and genomic studies, which suggested that the lacertid Z chromosome should be one of the smaller macrochromosomes.
AB - Reptiles are good objects for studying the evolution of sex determination, since they have different sex determination systems in different lineages. Lacertid lizards have been long-known for possessing ZZ/ ZW type sex chromosomes. However, due to morphological uniformity of lacertid chromosomes, the Z chromosome has been only putatively cytologically identified. We used lampbrush chromosome (LBC) analysis and FISH with a W-specific probe in Eremias velox (Pallas, 1771) to unequivocally identify the ZW bivalent and investigate its meiotic behavior. The heterochromatic W chromosome is decondensed at the lampbrush stage, indicating active transcription, contrast with the highly condensed condition of the lampbrush W chromosomes in birds. We identified the Z chromosome by its chiasmatic association with the W chromosome as chromosome XIII of the 19 chromosomes in the LBC karyotype. Our findings agree with previous genetic and genomic studies, which suggested that the lacertid Z chromosome should be one of the smaller macrochromosomes.
KW - Heterochromatin
KW - Lampbrush chromosomes
KW - Lizard
KW - Meiosis
KW - Microdissection
KW - Sex chromosomes
KW - RECOMBINATION
KW - GECKO
KW - meiosis
KW - sex chromosomes
KW - GENOME
KW - heterochromatin
KW - EVOLUTION
KW - lampbrush chromosomes
KW - DNA
KW - microdissection
KW - KARYOTYPE
KW - lizard
KW - INSIGHTS
UR - http://www.scopus.com/inward/record.url?scp=85067334464&partnerID=8YFLogxK
U2 - 10.3897/CompCytogen.v13i2.34116
DO - 10.3897/CompCytogen.v13i2.34116
M3 - Article
C2 - 31149328
AN - SCOPUS:85067334464
VL - 13
SP - 121
EP - 132
JO - Comparative Cytogenetics
JF - Comparative Cytogenetics
SN - 1993-0771
IS - 2
ER -
ID: 20588338