Standard

How a phase image of a cell with nucleus refractive index smaller than that of the cytoplasm should look like? A Comment on two papers by Steelman et al. and Schürmann et al. Read the Responses to this Comment: e201800091 and e201800095. / Yurkin, Maxim A.

в: Journal of Biophotonics, Том 11, № 6, e201800033, 01.06.2018.

Результаты исследований: Научные публикации в периодических изданиях › письмо/краткое сообщение › Рецензирование

Harvard

Yurkin, MA 2018, 'How a phase image of a cell with nucleus refractive index smaller than that of the cytoplasm should look like? A Comment on two papers by Steelman et al. and Schürmann et al. Read the Responses to this Comment: e201800091 and e201800095', Journal of Biophotonics, Том. 11, № 6, e201800033. https://doi.org/10.1002/jbio.201800033

APA

Yurkin, M. A. (2018). How a phase image of a cell with nucleus refractive index smaller than that of the cytoplasm should look like? A Comment on two papers by Steelman et al. and Schürmann et al. Read the Responses to this Comment: e201800091 and e201800095. Journal of Biophotonics, 11(6), [e201800033]. https://doi.org/10.1002/jbio.201800033

Vancouver

Yurkin MA. How a phase image of a cell with nucleus refractive index smaller than that of the cytoplasm should look like? A Comment on two papers by Steelman et al. and Schürmann et al. Read the Responses to this Comment: e201800091 and e201800095. Journal of Biophotonics. 2018 июнь 1;11(6):e201800033. doi: 10.1002/jbio.201800033

Author

Yurkin, Maxim A. / How a phase image of a cell with nucleus refractive index smaller than that of the cytoplasm should look like? A Comment on two papers by Steelman et al. and Schürmann et al. Read the Responses to this Comment: e201800091 and e201800095. в: Journal of Biophotonics. 2018 ; Том 11, № 6.

BibTeX

@article{580915f5df554555be6fed3049892357,
title = "How a phase image of a cell with nucleus refractive index smaller than that of the cytoplasm should look like?: A Comment on two papers by Steelman et al. and Sch{\"u}rmann et al. Read the Responses to this Comment: e201800091 and e201800095",
abstract = "In recent papers Steelman et al. ({"}Is the nuclear refractive index lower than cytoplasm? Validation of phase measurements and implications for light scattering technologies{"}) and Sch{\"u}rmann et al. ({"}Cell nuclei have lower refractive index and mass density than cytoplasm{"}) obtained quantitative phase images of whole cells of various types and corresponding isolated nuclei and concluded that the refractive index (RI) of the nucleus is significantly smaller than that of the cytoplasm. The comment shows that this conclusion and assumptions used in retrieving the RI necessarily imply a characteristic dip in the center of the whole-cell phase images. This dip is not present in any of the phase images in the discussed papers, which is a strong argument against the conclusion of smaller nucleus RI. It is also discussed whether a different processing of the phase images can help to clarify this issue.",
keywords = "Light scattering, Microscopy, Nucleus, Phase imaging, Refractive index, nucleus, light scattering, microscopy, refractive index, phase imaging, Refractometry, Cell Nucleus, Cytoplasm",
author = "Yurkin, {Maxim A.}",
note = "{\textcopyright} 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2018",
month = jun,
day = "1",
doi = "10.1002/jbio.201800033",
language = "English",
volume = "11",
journal = "Journal of Biophotonics",
issn = "1864-063X",
publisher = "Wiley-VCH Verlag",
number = "6",

}

RIS

TY - JOUR

T1 - How a phase image of a cell with nucleus refractive index smaller than that of the cytoplasm should look like?

T2 - A Comment on two papers by Steelman et al. and Schürmann et al. Read the Responses to this Comment: e201800091 and e201800095

AU - Yurkin, Maxim A.

N1 - © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2018/6/1

Y1 - 2018/6/1

N2 - In recent papers Steelman et al. ("Is the nuclear refractive index lower than cytoplasm? Validation of phase measurements and implications for light scattering technologies") and Schürmann et al. ("Cell nuclei have lower refractive index and mass density than cytoplasm") obtained quantitative phase images of whole cells of various types and corresponding isolated nuclei and concluded that the refractive index (RI) of the nucleus is significantly smaller than that of the cytoplasm. The comment shows that this conclusion and assumptions used in retrieving the RI necessarily imply a characteristic dip in the center of the whole-cell phase images. This dip is not present in any of the phase images in the discussed papers, which is a strong argument against the conclusion of smaller nucleus RI. It is also discussed whether a different processing of the phase images can help to clarify this issue.

AB - In recent papers Steelman et al. ("Is the nuclear refractive index lower than cytoplasm? Validation of phase measurements and implications for light scattering technologies") and Schürmann et al. ("Cell nuclei have lower refractive index and mass density than cytoplasm") obtained quantitative phase images of whole cells of various types and corresponding isolated nuclei and concluded that the refractive index (RI) of the nucleus is significantly smaller than that of the cytoplasm. The comment shows that this conclusion and assumptions used in retrieving the RI necessarily imply a characteristic dip in the center of the whole-cell phase images. This dip is not present in any of the phase images in the discussed papers, which is a strong argument against the conclusion of smaller nucleus RI. It is also discussed whether a different processing of the phase images can help to clarify this issue.

KW - Light scattering

KW - Microscopy

KW - Nucleus

KW - Phase imaging

KW - Refractive index

KW - nucleus

KW - light scattering

KW - microscopy

KW - refractive index

KW - phase imaging

KW - Refractometry

KW - Cell Nucleus

KW - Cytoplasm

UR - http://www.scopus.com/inward/record.url?scp=85046363137&partnerID=8YFLogxK

U2 - 10.1002/jbio.201800033

DO - 10.1002/jbio.201800033

M3 - Letter

C2 - 29722164

AN - SCOPUS:85046363137

VL - 11

JO - Journal of Biophotonics

JF - Journal of Biophotonics

SN - 1864-063X

IS - 6

M1 - e201800033

ER -

ID: 13072495