Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Genomic organization and physical mapping of tandemly arranged repetitive DNAs in Sterlet (Acipenser ruthenus). / Biltueva, Larisa S.; Prokopov, Dimitry Y.; Makunin, Alexey I. и др.
в: Cytogenetic and Genome Research, Том 152, № 3, 01.10.2017, стр. 148-157.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Genomic organization and physical mapping of tandemly arranged repetitive DNAs in Sterlet (Acipenser ruthenus)
AU - Biltueva, Larisa S.
AU - Prokopov, Dimitry Y.
AU - Makunin, Alexey I.
AU - Komissarov, Alexey S.
AU - Kudryavtseva, Anna V.
AU - Lemskaya, Natalya A.
AU - Vorobieva, Nadezhda V.
AU - Serdyukova, Natalia A.
AU - Romanenko, Svetlana A.
AU - Gladkikh, Olga L.
AU - Graphodatsky, Alexander S.
AU - Trifonov, Vladimir A.
N1 - © 2017 S. Karger AG, Basel.
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Acipenseriformes represent a phylogenetically basal clade of ray-finned fish characterized by unusual genomic traits, including paleopolyploid states of extant genomes with high chromosome numbers and slow rates of molecular evolution. Despite a high interest in this fish group, only a limited number of studies have been accomplished on the isolation and characterization of repetitive DNA, karyotype standardization is not yet complete, and sex chromosomes are still to be identified. Here, we applied next-generation sequencing and cluster analysis to characterize major fractions of sterlet (Acipenser ruthenus) repetitive DNA. Using FISH, we mapped 16 tandemly arranged sequences on sterlet chromosomes and found them to be unevenly distributed in the genome with a tendency to cluster in particular regions. Some of the satellite DNAs might be used as specific markers to identify individual chromosomes and their paralogs, resulting in the unequivocal identification of at least 18 chromosome pairs. Our results provide an insight into the characteristic genomic distribution of the most common sterlet repetitive sequences. Biased accumulation of repetitive DNAs in particular chromosomes makes them especially interesting for further search for cryptic sex chromosomes. Future studies of these sequences in other acipenserid species will provide new perspectives regarding the evolution of repetitive DNA within the genomes of this fish order.
AB - Acipenseriformes represent a phylogenetically basal clade of ray-finned fish characterized by unusual genomic traits, including paleopolyploid states of extant genomes with high chromosome numbers and slow rates of molecular evolution. Despite a high interest in this fish group, only a limited number of studies have been accomplished on the isolation and characterization of repetitive DNA, karyotype standardization is not yet complete, and sex chromosomes are still to be identified. Here, we applied next-generation sequencing and cluster analysis to characterize major fractions of sterlet (Acipenser ruthenus) repetitive DNA. Using FISH, we mapped 16 tandemly arranged sequences on sterlet chromosomes and found them to be unevenly distributed in the genome with a tendency to cluster in particular regions. Some of the satellite DNAs might be used as specific markers to identify individual chromosomes and their paralogs, resulting in the unequivocal identification of at least 18 chromosome pairs. Our results provide an insight into the characteristic genomic distribution of the most common sterlet repetitive sequences. Biased accumulation of repetitive DNAs in particular chromosomes makes them especially interesting for further search for cryptic sex chromosomes. Future studies of these sequences in other acipenserid species will provide new perspectives regarding the evolution of repetitive DNA within the genomes of this fish order.
KW - Acipenseridae
KW - DNA repeats
KW - FISH mapping
KW - Genome sequencing
KW - Sturgeon
KW - DNA, Satellite/genetics
KW - Microdissection
KW - Physical Chromosome Mapping
KW - In Situ Hybridization, Fluorescence
KW - Genetic Markers
KW - Fishes/genetics
KW - DNA, Ribosomal/genetics
KW - Sequence Analysis, DNA
KW - Animals
KW - Karyotyping
KW - Sex Chromosomes/genetics
KW - Evolution, Molecular
KW - CHARACIFORMES
KW - SATELLITE DNA
KW - ORDER ACIPENSERIFORMES
KW - RIBOSOMAL-RNA GENES
KW - PLOIDY LEVELS
KW - STURGEON GENETICS
KW - SEX-CHROMOSOME EVOLUTION
KW - CYTOGENETICS
KW - FISH
KW - DIFFERENTIATION
UR - http://www.scopus.com/inward/record.url?scp=85028753452&partnerID=8YFLogxK
U2 - 10.1159/000479472
DO - 10.1159/000479472
M3 - Article
C2 - 28850953
AN - SCOPUS:85028753452
VL - 152
SP - 148
EP - 157
JO - Cytogenetic and Genome Research
JF - Cytogenetic and Genome Research
SN - 1424-8581
IS - 3
ER -
ID: 9913413