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Genomic organization and physical mapping of tandemly arranged repetitive DNAs in Sterlet (Acipenser ruthenus). / Biltueva, Larisa S.; Prokopov, Dimitry Y.; Makunin, Alexey I. et al.

In: Cytogenetic and Genome Research, Vol. 152, No. 3, 01.10.2017, p. 148-157.

Research output: Contribution to journalArticlepeer-review

Harvard

Biltueva, LS, Prokopov, DY, Makunin, AI, Komissarov, AS, Kudryavtseva, AV, Lemskaya, NA, Vorobieva, NV, Serdyukova, NA, Romanenko, SA, Gladkikh, OL, Graphodatsky, AS & Trifonov, VA 2017, 'Genomic organization and physical mapping of tandemly arranged repetitive DNAs in Sterlet (Acipenser ruthenus)', Cytogenetic and Genome Research, vol. 152, no. 3, pp. 148-157. https://doi.org/10.1159/000479472

APA

Biltueva, L. S., Prokopov, D. Y., Makunin, A. I., Komissarov, A. S., Kudryavtseva, A. V., Lemskaya, N. A., Vorobieva, N. V., Serdyukova, N. A., Romanenko, S. A., Gladkikh, O. L., Graphodatsky, A. S., & Trifonov, V. A. (2017). Genomic organization and physical mapping of tandemly arranged repetitive DNAs in Sterlet (Acipenser ruthenus). Cytogenetic and Genome Research, 152(3), 148-157. https://doi.org/10.1159/000479472

Vancouver

Biltueva LS, Prokopov DY, Makunin AI, Komissarov AS, Kudryavtseva AV, Lemskaya NA et al. Genomic organization and physical mapping of tandemly arranged repetitive DNAs in Sterlet (Acipenser ruthenus). Cytogenetic and Genome Research. 2017 Oct 1;152(3):148-157. doi: 10.1159/000479472

Author

Biltueva, Larisa S. ; Prokopov, Dimitry Y. ; Makunin, Alexey I. et al. / Genomic organization and physical mapping of tandemly arranged repetitive DNAs in Sterlet (Acipenser ruthenus). In: Cytogenetic and Genome Research. 2017 ; Vol. 152, No. 3. pp. 148-157.

BibTeX

@article{db61ddf5047a4309b5eb07f605dc117e,
title = "Genomic organization and physical mapping of tandemly arranged repetitive DNAs in Sterlet (Acipenser ruthenus)",
abstract = "Acipenseriformes represent a phylogenetically basal clade of ray-finned fish characterized by unusual genomic traits, including paleopolyploid states of extant genomes with high chromosome numbers and slow rates of molecular evolution. Despite a high interest in this fish group, only a limited number of studies have been accomplished on the isolation and characterization of repetitive DNA, karyotype standardization is not yet complete, and sex chromosomes are still to be identified. Here, we applied next-generation sequencing and cluster analysis to characterize major fractions of sterlet (Acipenser ruthenus) repetitive DNA. Using FISH, we mapped 16 tandemly arranged sequences on sterlet chromosomes and found them to be unevenly distributed in the genome with a tendency to cluster in particular regions. Some of the satellite DNAs might be used as specific markers to identify individual chromosomes and their paralogs, resulting in the unequivocal identification of at least 18 chromosome pairs. Our results provide an insight into the characteristic genomic distribution of the most common sterlet repetitive sequences. Biased accumulation of repetitive DNAs in particular chromosomes makes them especially interesting for further search for cryptic sex chromosomes. Future studies of these sequences in other acipenserid species will provide new perspectives regarding the evolution of repetitive DNA within the genomes of this fish order.",
keywords = "Acipenseridae, DNA repeats, FISH mapping, Genome sequencing, Sturgeon, DNA, Satellite/genetics, Microdissection, Physical Chromosome Mapping, In Situ Hybridization, Fluorescence, Genetic Markers, Fishes/genetics, DNA, Ribosomal/genetics, Sequence Analysis, DNA, Animals, Karyotyping, Sex Chromosomes/genetics, Evolution, Molecular, CHARACIFORMES, SATELLITE DNA, ORDER ACIPENSERIFORMES, RIBOSOMAL-RNA GENES, PLOIDY LEVELS, STURGEON GENETICS, SEX-CHROMOSOME EVOLUTION, CYTOGENETICS, FISH, DIFFERENTIATION",
author = "Biltueva, {Larisa S.} and Prokopov, {Dimitry Y.} and Makunin, {Alexey I.} and Komissarov, {Alexey S.} and Kudryavtseva, {Anna V.} and Lemskaya, {Natalya A.} and Vorobieva, {Nadezhda V.} and Serdyukova, {Natalia A.} and Romanenko, {Svetlana A.} and Gladkikh, {Olga L.} and Graphodatsky, {Alexander S.} and Trifonov, {Vladimir A.}",
note = "{\textcopyright} 2017 S. Karger AG, Basel.",
year = "2017",
month = oct,
day = "1",
doi = "10.1159/000479472",
language = "English",
volume = "152",
pages = "148--157",
journal = "Cytogenetic and Genome Research",
issn = "1424-8581",
publisher = "S. Karger AG",
number = "3",

}

RIS

TY - JOUR

T1 - Genomic organization and physical mapping of tandemly arranged repetitive DNAs in Sterlet (Acipenser ruthenus)

AU - Biltueva, Larisa S.

AU - Prokopov, Dimitry Y.

AU - Makunin, Alexey I.

AU - Komissarov, Alexey S.

AU - Kudryavtseva, Anna V.

AU - Lemskaya, Natalya A.

AU - Vorobieva, Nadezhda V.

AU - Serdyukova, Natalia A.

AU - Romanenko, Svetlana A.

AU - Gladkikh, Olga L.

AU - Graphodatsky, Alexander S.

AU - Trifonov, Vladimir A.

N1 - © 2017 S. Karger AG, Basel.

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Acipenseriformes represent a phylogenetically basal clade of ray-finned fish characterized by unusual genomic traits, including paleopolyploid states of extant genomes with high chromosome numbers and slow rates of molecular evolution. Despite a high interest in this fish group, only a limited number of studies have been accomplished on the isolation and characterization of repetitive DNA, karyotype standardization is not yet complete, and sex chromosomes are still to be identified. Here, we applied next-generation sequencing and cluster analysis to characterize major fractions of sterlet (Acipenser ruthenus) repetitive DNA. Using FISH, we mapped 16 tandemly arranged sequences on sterlet chromosomes and found them to be unevenly distributed in the genome with a tendency to cluster in particular regions. Some of the satellite DNAs might be used as specific markers to identify individual chromosomes and their paralogs, resulting in the unequivocal identification of at least 18 chromosome pairs. Our results provide an insight into the characteristic genomic distribution of the most common sterlet repetitive sequences. Biased accumulation of repetitive DNAs in particular chromosomes makes them especially interesting for further search for cryptic sex chromosomes. Future studies of these sequences in other acipenserid species will provide new perspectives regarding the evolution of repetitive DNA within the genomes of this fish order.

AB - Acipenseriformes represent a phylogenetically basal clade of ray-finned fish characterized by unusual genomic traits, including paleopolyploid states of extant genomes with high chromosome numbers and slow rates of molecular evolution. Despite a high interest in this fish group, only a limited number of studies have been accomplished on the isolation and characterization of repetitive DNA, karyotype standardization is not yet complete, and sex chromosomes are still to be identified. Here, we applied next-generation sequencing and cluster analysis to characterize major fractions of sterlet (Acipenser ruthenus) repetitive DNA. Using FISH, we mapped 16 tandemly arranged sequences on sterlet chromosomes and found them to be unevenly distributed in the genome with a tendency to cluster in particular regions. Some of the satellite DNAs might be used as specific markers to identify individual chromosomes and their paralogs, resulting in the unequivocal identification of at least 18 chromosome pairs. Our results provide an insight into the characteristic genomic distribution of the most common sterlet repetitive sequences. Biased accumulation of repetitive DNAs in particular chromosomes makes them especially interesting for further search for cryptic sex chromosomes. Future studies of these sequences in other acipenserid species will provide new perspectives regarding the evolution of repetitive DNA within the genomes of this fish order.

KW - Acipenseridae

KW - DNA repeats

KW - FISH mapping

KW - Genome sequencing

KW - Sturgeon

KW - DNA, Satellite/genetics

KW - Microdissection

KW - Physical Chromosome Mapping

KW - In Situ Hybridization, Fluorescence

KW - Genetic Markers

KW - Fishes/genetics

KW - DNA, Ribosomal/genetics

KW - Sequence Analysis, DNA

KW - Animals

KW - Karyotyping

KW - Sex Chromosomes/genetics

KW - Evolution, Molecular

KW - CHARACIFORMES

KW - SATELLITE DNA

KW - ORDER ACIPENSERIFORMES

KW - RIBOSOMAL-RNA GENES

KW - PLOIDY LEVELS

KW - STURGEON GENETICS

KW - SEX-CHROMOSOME EVOLUTION

KW - CYTOGENETICS

KW - FISH

KW - DIFFERENTIATION

UR - http://www.scopus.com/inward/record.url?scp=85028753452&partnerID=8YFLogxK

U2 - 10.1159/000479472

DO - 10.1159/000479472

M3 - Article

C2 - 28850953

AN - SCOPUS:85028753452

VL - 152

SP - 148

EP - 157

JO - Cytogenetic and Genome Research

JF - Cytogenetic and Genome Research

SN - 1424-8581

IS - 3

ER -

ID: 9913413