TY - JOUR

T1 - Genetic diversity of pinus sibirica, P. pumila and their natural hybrids based on non-linked nuclear loci

AU - Vasilyeva, Galina

AU - Vavilova, Valeriya

AU - Ustyantsev, Kirill

AU - Sukhikh, Igor

AU - Blinov, Alexander

AU - Goroshkevich, Sergei

AU - Sokolov, Victor

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Frequent discordant phylogenies inferred from different loci, as well as the presence of sufficiently diverged gene variants within a single species isolate are indicative of potentially frequent non-monophyly in the genus Pinus. Interspecies hybridisation and incomplete lineage sorting have been suggested as possible explanations for the observed phylogenetic discrepancies. However, there is no direct evidence to support any of the proposed scenarios for the Eurasian five-needle pines. We used natural hybrids between Pinus sibirica and P. pumila, as well as their parental species, as a model to reproduce the scenario of non-monophyly in the subgenus Strobus. Three non-linked nuclear DNA loci (LEA, AGP6 and 4CL) were applied to detect introgressive alleles and to genetically discriminate the studied species. Comparative sequence analyses revealed two clusters of species-specific alleles for each of the markers, characteristic for either P. sibirica or P. pumila. No hybrid-specific alleles were found. We also found no hybrids with a genotype characteristic of only one of the parental species for all three loci. On average, the hybrids were characterised by an equal ratio of alleles from the P. sibirica and P. pumila clusters. We reveal that some trees of pure species originating from allopatric locations have non-specific loci that can be a result of genetic exchange between these species in the distant past or incomplete lineage sorting.

AB - Frequent discordant phylogenies inferred from different loci, as well as the presence of sufficiently diverged gene variants within a single species isolate are indicative of potentially frequent non-monophyly in the genus Pinus. Interspecies hybridisation and incomplete lineage sorting have been suggested as possible explanations for the observed phylogenetic discrepancies. However, there is no direct evidence to support any of the proposed scenarios for the Eurasian five-needle pines. We used natural hybrids between Pinus sibirica and P. pumila, as well as their parental species, as a model to reproduce the scenario of non-monophyly in the subgenus Strobus. Three non-linked nuclear DNA loci (LEA, AGP6 and 4CL) were applied to detect introgressive alleles and to genetically discriminate the studied species. Comparative sequence analyses revealed two clusters of species-specific alleles for each of the markers, characteristic for either P. sibirica or P. pumila. No hybrid-specific alleles were found. We also found no hybrids with a genotype characteristic of only one of the parental species for all three loci. On average, the hybrids were characterised by an equal ratio of alleles from the P. sibirica and P. pumila clusters. We reveal that some trees of pure species originating from allopatric locations have non-specific loci that can be a result of genetic exchange between these species in the distant past or incomplete lineage sorting.

KW - Genetic variation

KW - Hybridisation

KW - Siberian dwarf pine

KW - Siberian stone pine

KW - STROBUS

KW - FRAGMENT

KW - genetic variation

KW - HYBRIDIZATION

KW - PINES

KW - hybridisation

KW - SOFTWARE

UR - http://www.scopus.com/inward/record.url?scp=85049804505&partnerID=8YFLogxK

U2 - 10.12657/denbio.079.015

DO - 10.12657/denbio.079.015

M3 - Article

AN - SCOPUS:85049804505

VL - 79

SP - 168

EP - 173

JO - Dendrobiology

JF - Dendrobiology

SN - 1641-1307

ER -