Genetic diversity of pinus sibirica, P. pumila and their natural hybrids based on non-linked nuclear loci. / Vasilyeva, Galina; Vavilova, Valeriya; Ustyantsev, Kirill et al.
In: Dendrobiology, Vol. 79, 01.01.2018, p. 168-173.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Genetic diversity of pinus sibirica, P. pumila and their natural hybrids based on non-linked nuclear loci
AU - Vasilyeva, Galina
AU - Vavilova, Valeriya
AU - Ustyantsev, Kirill
AU - Sukhikh, Igor
AU - Blinov, Alexander
AU - Goroshkevich, Sergei
AU - Sokolov, Victor
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Frequent discordant phylogenies inferred from different loci, as well as the presence of sufficiently diverged gene variants within a single species isolate are indicative of potentially frequent non-monophyly in the genus Pinus. Interspecies hybridisation and incomplete lineage sorting have been suggested as possible explanations for the observed phylogenetic discrepancies. However, there is no direct evidence to support any of the proposed scenarios for the Eurasian five-needle pines. We used natural hybrids between Pinus sibirica and P. pumila, as well as their parental species, as a model to reproduce the scenario of non-monophyly in the subgenus Strobus. Three non-linked nuclear DNA loci (LEA, AGP6 and 4CL) were applied to detect introgressive alleles and to genetically discriminate the studied species. Comparative sequence analyses revealed two clusters of species-specific alleles for each of the markers, characteristic for either P. sibirica or P. pumila. No hybrid-specific alleles were found. We also found no hybrids with a genotype characteristic of only one of the parental species for all three loci. On average, the hybrids were characterised by an equal ratio of alleles from the P. sibirica and P. pumila clusters. We reveal that some trees of pure species originating from allopatric locations have non-specific loci that can be a result of genetic exchange between these species in the distant past or incomplete lineage sorting.
AB - Frequent discordant phylogenies inferred from different loci, as well as the presence of sufficiently diverged gene variants within a single species isolate are indicative of potentially frequent non-monophyly in the genus Pinus. Interspecies hybridisation and incomplete lineage sorting have been suggested as possible explanations for the observed phylogenetic discrepancies. However, there is no direct evidence to support any of the proposed scenarios for the Eurasian five-needle pines. We used natural hybrids between Pinus sibirica and P. pumila, as well as their parental species, as a model to reproduce the scenario of non-monophyly in the subgenus Strobus. Three non-linked nuclear DNA loci (LEA, AGP6 and 4CL) were applied to detect introgressive alleles and to genetically discriminate the studied species. Comparative sequence analyses revealed two clusters of species-specific alleles for each of the markers, characteristic for either P. sibirica or P. pumila. No hybrid-specific alleles were found. We also found no hybrids with a genotype characteristic of only one of the parental species for all three loci. On average, the hybrids were characterised by an equal ratio of alleles from the P. sibirica and P. pumila clusters. We reveal that some trees of pure species originating from allopatric locations have non-specific loci that can be a result of genetic exchange between these species in the distant past or incomplete lineage sorting.
KW - Genetic variation
KW - Hybridisation
KW - Siberian dwarf pine
KW - Siberian stone pine
KW - STROBUS
KW - FRAGMENT
KW - genetic variation
KW - HYBRIDIZATION
KW - PINES
KW - hybridisation
KW - SOFTWARE
UR - http://www.scopus.com/inward/record.url?scp=85049804505&partnerID=8YFLogxK
U2 - 10.12657/denbio.079.015
DO - 10.12657/denbio.079.015
M3 - Article
AN - SCOPUS:85049804505
VL - 79
SP - 168
EP - 173
JO - Dendrobiology
JF - Dendrobiology
SN - 1641-1307
ER -
ID: 23267067