Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Generation of two iPSC lines (IMGTi001-A and IMGTi001-B) from human skin fibroblasts with ring chromosome 22. / Nikitina, T. V.; Menzorov, A. G.; Kashevarova, A. A. и др.
в: Stem Cell Research, Том 31, 01.08.2018, стр. 244-248.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Generation of two iPSC lines (IMGTi001-A and IMGTi001-B) from human skin fibroblasts with ring chromosome 22
AU - Nikitina, T. V.
AU - Menzorov, A. G.
AU - Kashevarova, A. A.
AU - Gridina, M. M.
AU - Khabarova, A. A.
AU - Yakovleva, Yu S.
AU - Lopatkina, M. E.
AU - Kizilova, E. A.
AU - Vasilyev, S. A.
AU - Serov, O. L.
AU - Lebedev, I. N.
N1 - Publisher Copyright: © 2018 The Authors
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Skin fibroblasts from a patient with intellectual disability and ring chromosome 22 were reprogrammed into induced pluripotent stem cells (iPSCs) to establish a clonal stem cell lines, IMGTi001-A (iTAF5-29) and IMGTi001-B (iTAF5-32). Because of ring chromosome mitotic instability these cell lines show mosaic karyotypes with 46,XX,r(22) in >83% cells, 45,XX,-22 as minor class and sporadically cells with other karyotypes. Differentiation in derivatives of all three germ layers was shown in teratoma assay for IMGTi001-A, and in embryoid bodies for both cell lines. To our knowledge, human iPSC lines with ring chromosome are described for the first time.
AB - Skin fibroblasts from a patient with intellectual disability and ring chromosome 22 were reprogrammed into induced pluripotent stem cells (iPSCs) to establish a clonal stem cell lines, IMGTi001-A (iTAF5-29) and IMGTi001-B (iTAF5-32). Because of ring chromosome mitotic instability these cell lines show mosaic karyotypes with 46,XX,r(22) in >83% cells, 45,XX,-22 as minor class and sporadically cells with other karyotypes. Differentiation in derivatives of all three germ layers was shown in teratoma assay for IMGTi001-A, and in embryoid bodies for both cell lines. To our knowledge, human iPSC lines with ring chromosome are described for the first time.
KW - Child, Preschool
KW - Female
KW - Fibroblasts/metabolism
KW - Humans
KW - Induced Pluripotent Stem Cells/metabolism
KW - Ring Chromosomes
KW - Skin/growth & development
UR - http://www.scopus.com/inward/record.url?scp=85051820165&partnerID=8YFLogxK
U2 - 10.1016/j.scr.2018.08.012
DO - 10.1016/j.scr.2018.08.012
M3 - Article
C2 - 30144655
AN - SCOPUS:85051820165
VL - 31
SP - 244
EP - 248
JO - Stem Cell Research
JF - Stem Cell Research
SN - 1873-5061
ER -
ID: 16112257