TY - JOUR

T1 - Food, drug and model mix analysis by organic monolithic columns based on heterocyclic monomers

AU - Sotnikova, Yulia S.

AU - Patrushev, Yuri V.

N1 - This work was supported Ministry of Science and Higher Education of the Russian Federation within the governmental order for Boreskov Institute of Catalysis (project АААА-А21-121011390053-4).

PY - 2023

Y1 - 2023

N2 - This paper presents examples of separation on monolithic columns prepared by two different methods: copolymerization and post-modification. In the first method, monoliths were prepared from styrene, divinylbenzene, and heterocyclic nitrogen-containing monomers. In the second, a styrene/divinylbenzene/4-vinylbenzyl chloride copolymer was obtained and in situ modified with 1-methylimidazole. It has been shown that monolithic columns are capable of separating macromolecules and low-molecular compounds. A comparison was made between a styrene/divinylbenzene/1-vinylimidazole copolymer column and a ProSwift™ RP-3U monolithic column. It has been established that a monolithic column based on 1-vinylimidazole is capable of separating proteins and low molecular weight test substances. However, the ProSwift™ RP-3U column is only capable of separating proteins. Separation examples are given: a test mixture of proteins, natural milk proteins, carbohydrates and drugs on a column with a sorbent based on styrene/divinylbenzene. A modified column was used to study the content of salicylic acid in medicinal preparations containing acetylsalicylic acid.

AB - This paper presents examples of separation on monolithic columns prepared by two different methods: copolymerization and post-modification. In the first method, monoliths were prepared from styrene, divinylbenzene, and heterocyclic nitrogen-containing monomers. In the second, a styrene/divinylbenzene/4-vinylbenzyl chloride copolymer was obtained and in situ modified with 1-methylimidazole. It has been shown that monolithic columns are capable of separating macromolecules and low-molecular compounds. A comparison was made between a styrene/divinylbenzene/1-vinylimidazole copolymer column and a ProSwift™ RP-3U monolithic column. It has been established that a monolithic column based on 1-vinylimidazole is capable of separating proteins and low molecular weight test substances. However, the ProSwift™ RP-3U column is only capable of separating proteins. Separation examples are given: a test mixture of proteins, natural milk proteins, carbohydrates and drugs on a column with a sorbent based on styrene/divinylbenzene. A modified column was used to study the content of salicylic acid in medicinal preparations containing acetylsalicylic acid.

KW - HPLC

KW - macromolecules separating

KW - mixed-mode chromatography

KW - monolithic columns

KW - porous polymer

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85165259456&origin=inward&txGid=b14f19d3d6abf47ac1b317a0778d7c29

UR - https://www.mendeley.com/catalogue/1282b78c-36b7-3048-a4f9-98d75b34835f/

U2 - 10.1080/10826076.2023.2235402

DO - 10.1080/10826076.2023.2235402

M3 - Article

VL - 46

SP - 211

EP - 217

JO - Journal of Liquid Chromatography and Related Technologies

JF - Journal of Liquid Chromatography and Related Technologies

SN - 1082-6076

IS - 11-15

ER -