Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Expression of the miR-190 family is increased under DDT exposure in vivo and in vitro. / Kalinina, Tatiana S.; Kononchuk, Vladislav V.; Ovchinnikov, Vladimir Y. и др.
в: Molecular Biology Reports, Том 45, № 6, 01.12.2018, стр. 1937-1945.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Expression of the miR-190 family is increased under DDT exposure in vivo and in vitro
AU - Kalinina, Tatiana S.
AU - Kononchuk, Vladislav V.
AU - Ovchinnikov, Vladimir Y.
AU - Chanyshev, Mikhail D.
AU - Gulyaeva, Lyudmila F.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - A non-genotoxic insecticide dichlorodiphenyltrichloroethane (DDT), can affect mRNA and microRNA levels, however, its precise mechanism of action remains poorly understood. Using in silico methods we found that the rat miR-190 family is potentially regulated by CAR and ER receptors activated by DDT. We showed that exposure to DDT results in a dose- and organ-dependent increase in the expression of miR-190a, -190b in the liver, uterus, ovaries and mammary gland of female Wistar rats. Additionally, we demonstrate a decrease in protein product level of Tp53inp1, the target gene of these microRNAs, in the rat uterus. It is known that miR-190 is probably regulated by ER in humans, thus we measured the level of miR-190a, -190b in primary cultures of malignant and normal human endometrial cells treated with different doses of DDT. We detected an increase in miR-190b level in normal endometrial cells under DDT exposure. Thus, our results indicate that DDT exposure lead to change in the expression of oncogenic miR-190 family and its target gene Tp53inp1 which may be due to activation of CAR and ER.
AB - A non-genotoxic insecticide dichlorodiphenyltrichloroethane (DDT), can affect mRNA and microRNA levels, however, its precise mechanism of action remains poorly understood. Using in silico methods we found that the rat miR-190 family is potentially regulated by CAR and ER receptors activated by DDT. We showed that exposure to DDT results in a dose- and organ-dependent increase in the expression of miR-190a, -190b in the liver, uterus, ovaries and mammary gland of female Wistar rats. Additionally, we demonstrate a decrease in protein product level of Tp53inp1, the target gene of these microRNAs, in the rat uterus. It is known that miR-190 is probably regulated by ER in humans, thus we measured the level of miR-190a, -190b in primary cultures of malignant and normal human endometrial cells treated with different doses of DDT. We detected an increase in miR-190b level in normal endometrial cells under DDT exposure. Thus, our results indicate that DDT exposure lead to change in the expression of oncogenic miR-190 family and its target gene Tp53inp1 which may be due to activation of CAR and ER.
KW - Constitutive androstane receptor
KW - Dichlorodiphenyltrichloroethane
KW - Estrogen receptor
KW - MicroRNA
KW - Xenobiotics
KW - PROFILES
KW - SMALL RNAS
KW - TUMORS
KW - PESTICIDES
KW - BREAST-CANCER
KW - ROLES
KW - SAMPLES
KW - RECEPTORS
KW - POLYCHLORINATED-BIPHENYLS
KW - MICRORNA
UR - http://www.scopus.com/inward/record.url?scp=85056319564&partnerID=8YFLogxK
U2 - 10.1007/s11033-018-4343-0
DO - 10.1007/s11033-018-4343-0
M3 - Article
C2 - 30421125
AN - SCOPUS:85056319564
VL - 45
SP - 1937
EP - 1945
JO - Molecular Biology Reports
JF - Molecular Biology Reports
SN - 0301-4851
IS - 6
ER -
ID: 17488301