Standard

Expression of the miR-190 family is increased under DDT exposure in vivo and in vitro. / Kalinina, Tatiana S.; Kononchuk, Vladislav V.; Ovchinnikov, Vladimir Y. et al.

In: Molecular Biology Reports, Vol. 45, No. 6, 01.12.2018, p. 1937-1945.

Research output: Contribution to journal › Article › peer-review

Harvard

Kalinina, TS, Kononchuk, VV, Ovchinnikov, VY, Chanyshev, MD & Gulyaeva, LF 2018, 'Expression of the miR-190 family is increased under DDT exposure in vivo and in vitro', Molecular Biology Reports, vol. 45, no. 6, pp. 1937-1945. https://doi.org/10.1007/s11033-018-4343-0

APA

Kalinina, T. S., Kononchuk, V. V., Ovchinnikov, V. Y., Chanyshev, M. D., & Gulyaeva, L. F. (2018). Expression of the miR-190 family is increased under DDT exposure in vivo and in vitro. Molecular Biology Reports, 45(6), 1937-1945. https://doi.org/10.1007/s11033-018-4343-0

Vancouver

Kalinina TS, Kononchuk VV, Ovchinnikov VY, Chanyshev MD, Gulyaeva LF. Expression of the miR-190 family is increased under DDT exposure in vivo and in vitro. Molecular Biology Reports. 2018 Dec 1;45(6):1937-1945. doi: 10.1007/s11033-018-4343-0

Author

Kalinina, Tatiana S. ; Kononchuk, Vladislav V. ; Ovchinnikov, Vladimir Y. et al. / Expression of the miR-190 family is increased under DDT exposure in vivo and in vitro. In: Molecular Biology Reports. 2018 ; Vol. 45, No. 6. pp. 1937-1945.

BibTeX

@article{71e8fe074d5d4ee7a74fce610b8a38ce,
title = "Expression of the miR-190 family is increased under DDT exposure in vivo and in vitro",
abstract = "A non-genotoxic insecticide dichlorodiphenyltrichloroethane (DDT), can affect mRNA and microRNA levels, however, its precise mechanism of action remains poorly understood. Using in silico methods we found that the rat miR-190 family is potentially regulated by CAR and ER receptors activated by DDT. We showed that exposure to DDT results in a dose- and organ-dependent increase in the expression of miR-190a, -190b in the liver, uterus, ovaries and mammary gland of female Wistar rats. Additionally, we demonstrate a decrease in protein product level of Tp53inp1, the target gene of these microRNAs, in the rat uterus. It is known that miR-190 is probably regulated by ER in humans, thus we measured the level of miR-190a, -190b in primary cultures of malignant and normal human endometrial cells treated with different doses of DDT. We detected an increase in miR-190b level in normal endometrial cells under DDT exposure. Thus, our results indicate that DDT exposure lead to change in the expression of oncogenic miR-190 family and its target gene Tp53inp1 which may be due to activation of CAR and ER.",
keywords = "Constitutive androstane receptor, Dichlorodiphenyltrichloroethane, Estrogen receptor, MicroRNA, Xenobiotics, PROFILES, SMALL RNAS, TUMORS, PESTICIDES, BREAST-CANCER, ROLES, SAMPLES, RECEPTORS, POLYCHLORINATED-BIPHENYLS, MICRORNA",
author = "Kalinina, {Tatiana S.} and Kononchuk, {Vladislav V.} and Ovchinnikov, {Vladimir Y.} and Chanyshev, {Mikhail D.} and Gulyaeva, {Lyudmila F.}",
year = "2018",
month = dec,
day = "1",
doi = "10.1007/s11033-018-4343-0",
language = "English",
volume = "45",
pages = "1937--1945",
journal = "Molecular Biology Reports",
issn = "0301-4851",
publisher = "Springer Netherlands",
number = "6",

}

RIS

TY - JOUR

T1 - Expression of the miR-190 family is increased under DDT exposure in vivo and in vitro

AU - Kalinina, Tatiana S.

AU - Kononchuk, Vladislav V.

AU - Ovchinnikov, Vladimir Y.

AU - Chanyshev, Mikhail D.

AU - Gulyaeva, Lyudmila F.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - A non-genotoxic insecticide dichlorodiphenyltrichloroethane (DDT), can affect mRNA and microRNA levels, however, its precise mechanism of action remains poorly understood. Using in silico methods we found that the rat miR-190 family is potentially regulated by CAR and ER receptors activated by DDT. We showed that exposure to DDT results in a dose- and organ-dependent increase in the expression of miR-190a, -190b in the liver, uterus, ovaries and mammary gland of female Wistar rats. Additionally, we demonstrate a decrease in protein product level of Tp53inp1, the target gene of these microRNAs, in the rat uterus. It is known that miR-190 is probably regulated by ER in humans, thus we measured the level of miR-190a, -190b in primary cultures of malignant and normal human endometrial cells treated with different doses of DDT. We detected an increase in miR-190b level in normal endometrial cells under DDT exposure. Thus, our results indicate that DDT exposure lead to change in the expression of oncogenic miR-190 family and its target gene Tp53inp1 which may be due to activation of CAR and ER.

AB - A non-genotoxic insecticide dichlorodiphenyltrichloroethane (DDT), can affect mRNA and microRNA levels, however, its precise mechanism of action remains poorly understood. Using in silico methods we found that the rat miR-190 family is potentially regulated by CAR and ER receptors activated by DDT. We showed that exposure to DDT results in a dose- and organ-dependent increase in the expression of miR-190a, -190b in the liver, uterus, ovaries and mammary gland of female Wistar rats. Additionally, we demonstrate a decrease in protein product level of Tp53inp1, the target gene of these microRNAs, in the rat uterus. It is known that miR-190 is probably regulated by ER in humans, thus we measured the level of miR-190a, -190b in primary cultures of malignant and normal human endometrial cells treated with different doses of DDT. We detected an increase in miR-190b level in normal endometrial cells under DDT exposure. Thus, our results indicate that DDT exposure lead to change in the expression of oncogenic miR-190 family and its target gene Tp53inp1 which may be due to activation of CAR and ER.

KW - Constitutive androstane receptor

KW - Dichlorodiphenyltrichloroethane

KW - Estrogen receptor

KW - MicroRNA

KW - Xenobiotics

KW - PROFILES

KW - SMALL RNAS

KW - TUMORS

KW - PESTICIDES

KW - BREAST-CANCER

KW - ROLES

KW - SAMPLES

KW - RECEPTORS

KW - POLYCHLORINATED-BIPHENYLS

KW - MICRORNA

UR - http://www.scopus.com/inward/record.url?scp=85056319564&partnerID=8YFLogxK

U2 - 10.1007/s11033-018-4343-0

DO - 10.1007/s11033-018-4343-0

M3 - Article

C2 - 30421125

AN - SCOPUS:85056319564

VL - 45

SP - 1937

EP - 1945

JO - Molecular Biology Reports

JF - Molecular Biology Reports

SN - 0301-4851

IS - 6

ER -

ID: 17488301