Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Experimental study of the topological flow transformations in an aerial vortex bioreactor with a floating washer. / Naumov, Igor V; Gevorgiz, Ruslan G; Skripkin, Sergey G и др.
в: Biotechnology journal, Том 18, № 8, e2200644, 08.2023.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Experimental study of the topological flow transformations in an aerial vortex bioreactor with a floating washer
AU - Naumov, Igor V
AU - Gevorgiz, Ruslan G
AU - Skripkin, Sergey G
AU - Tintulova, Maria V
AU - Tsoi, Mikhail A
AU - Sharifullin, Bulat R
N1 - This research was funded by the Russian Science Foundation, grant number 19‐19‐00083. The manuscript does not contain human studies or experiments using animals. This article is protected by copyright. All rights reserved. © 2023 Wiley-VCH GmbH.
PY - 2023/8
Y1 - 2023/8
N2 - BACKGROUND: Research into the flow structure in an aerial vortex bioreactor is relevant for developing the methods for growing cell cultures. Determining the optimal cultivation condition for a certain process is especially important in the case when such parameters of the medium as density and viscosity significantly change with the culture growth in the bioreactor.METHODS AND RESULTS: The research of the flow dynamic was carried out in an 8.5 L universal aerial vortex bioreactor, with a washer freely floating on its surface and stabilizing the motion of the working fluid. The regularities of the vortex motion of the culture medium have been determined by Particle Image Velocimetry depending on its volume and the intensity of rotation of the activator, generating vortex motion in the air.CONCLUSION: The observed vortex structure and its dynamics at increasing flow swirl intensity are established to coincide with the structure of a confined vortex flow in a cylindrical container with no washer for both single and two-fluid configurations. This novel methodology of the flow optimization shows that an ascending swirling jet forms in the vicinity of the bioreactor axis, and a bubble-like vortex breakdown forms in the axial region. This article is protected by copyright. All rights reserved.
AB - BACKGROUND: Research into the flow structure in an aerial vortex bioreactor is relevant for developing the methods for growing cell cultures. Determining the optimal cultivation condition for a certain process is especially important in the case when such parameters of the medium as density and viscosity significantly change with the culture growth in the bioreactor.METHODS AND RESULTS: The research of the flow dynamic was carried out in an 8.5 L universal aerial vortex bioreactor, with a washer freely floating on its surface and stabilizing the motion of the working fluid. The regularities of the vortex motion of the culture medium have been determined by Particle Image Velocimetry depending on its volume and the intensity of rotation of the activator, generating vortex motion in the air.CONCLUSION: The observed vortex structure and its dynamics at increasing flow swirl intensity are established to coincide with the structure of a confined vortex flow in a cylindrical container with no washer for both single and two-fluid configurations. This novel methodology of the flow optimization shows that an ascending swirling jet forms in the vicinity of the bioreactor axis, and a bubble-like vortex breakdown forms in the axial region. This article is protected by copyright. All rights reserved.
KW - aerial bioreactor
KW - complex vortex
KW - marine microalgae
KW - vortex flow modeling
KW - vortex flows
KW - Bioreactors
KW - Rheology
KW - Cell Culture Techniques
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85159701996&origin=inward&txGid=b04488dcc3d34ed4d19e027541ccdaff
UR - https://www.mendeley.com/catalogue/21c43cf5-ace1-3554-9f9a-e9a5089766fd/
U2 - 10.1002/biot.202200644
DO - 10.1002/biot.202200644
M3 - Article
C2 - 37164941
VL - 18
JO - Biotechnology journal
JF - Biotechnology journal
SN - 1860-6768
IS - 8
M1 - e2200644
ER -
ID: 49496674