TY - JOUR

T1 - Experimental study of the topological flow transformations in an aerial vortex bioreactor with a floating washer

AU - Naumov, Igor V

AU - Gevorgiz, Ruslan G

AU - Skripkin, Sergey G

AU - Tintulova, Maria V

AU - Tsoi, Mikhail A

AU - Sharifullin, Bulat R

N1 - This research was funded by the Russian Science Foundation, grant number 19‐19‐00083. The manuscript does not contain human studies or experiments using animals. This article is protected by copyright. All rights reserved. © 2023 Wiley-VCH GmbH.

PY - 2023/8

Y1 - 2023/8

N2 - BACKGROUND: Research into the flow structure in an aerial vortex bioreactor is relevant for developing the methods for growing cell cultures. Determining the optimal cultivation condition for a certain process is especially important in the case when such parameters of the medium as density and viscosity significantly change with the culture growth in the bioreactor.METHODS AND RESULTS: The research of the flow dynamic was carried out in an 8.5 L universal aerial vortex bioreactor, with a washer freely floating on its surface and stabilizing the motion of the working fluid. The regularities of the vortex motion of the culture medium have been determined by Particle Image Velocimetry depending on its volume and the intensity of rotation of the activator, generating vortex motion in the air.CONCLUSION: The observed vortex structure and its dynamics at increasing flow swirl intensity are established to coincide with the structure of a confined vortex flow in a cylindrical container with no washer for both single and two-fluid configurations. This novel methodology of the flow optimization shows that an ascending swirling jet forms in the vicinity of the bioreactor axis, and a bubble-like vortex breakdown forms in the axial region. This article is protected by copyright. All rights reserved.

AB - BACKGROUND: Research into the flow structure in an aerial vortex bioreactor is relevant for developing the methods for growing cell cultures. Determining the optimal cultivation condition for a certain process is especially important in the case when such parameters of the medium as density and viscosity significantly change with the culture growth in the bioreactor.METHODS AND RESULTS: The research of the flow dynamic was carried out in an 8.5 L universal aerial vortex bioreactor, with a washer freely floating on its surface and stabilizing the motion of the working fluid. The regularities of the vortex motion of the culture medium have been determined by Particle Image Velocimetry depending on its volume and the intensity of rotation of the activator, generating vortex motion in the air.CONCLUSION: The observed vortex structure and its dynamics at increasing flow swirl intensity are established to coincide with the structure of a confined vortex flow in a cylindrical container with no washer for both single and two-fluid configurations. This novel methodology of the flow optimization shows that an ascending swirling jet forms in the vicinity of the bioreactor axis, and a bubble-like vortex breakdown forms in the axial region. This article is protected by copyright. All rights reserved.

KW - aerial bioreactor

KW - complex vortex

KW - marine microalgae

KW - vortex flow modeling

KW - vortex flows

KW - Bioreactors

KW - Rheology

KW - Cell Culture Techniques

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85159701996&origin=inward&txGid=b04488dcc3d34ed4d19e027541ccdaff

UR - https://www.mendeley.com/catalogue/21c43cf5-ace1-3554-9f9a-e9a5089766fd/

U2 - 10.1002/biot.202200644

DO - 10.1002/biot.202200644

M3 - Article

C2 - 37164941

VL - 18

JO - Biotechnology journal

JF - Biotechnology journal

SN - 1860-6768

IS - 8

M1 - e2200644

ER -