Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Evaluation of Advantages of Lithium Chloride as a Precipitating Agent in RNA Isolation from Frozen Vein Segments. / Korolenya, V. A.; Gavrilov, K. A.; Sevost’ianova, K. S. и др.
в: Bulletin of Experimental Biology and Medicine, Том 173, № 3, 07.2022, стр. 384-389.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Evaluation of Advantages of Lithium Chloride as a Precipitating Agent in RNA Isolation from Frozen Vein Segments
AU - Korolenya, V. A.
AU - Gavrilov, K. A.
AU - Sevost’ianova, K. S.
AU - Shevela, A. I.
AU - Filipenko, M. L.
AU - Smetanina, M. A.
N1 - Publisher Copyright: © 2022, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022/7
Y1 - 2022/7
N2 - Contamination of the sample with DNA is a problem when isolating RNA by phenol-chloroform extraction, and DNase treatment introduces an additional error in the analysis of gene expression. Bearing in mind the ability of LiCl to precipitate RNA selectively, we studied the possibility and advantage of using LiCl as a precipitation agent in the protocol for RNA precipitation [3] from frozen segments of large vessels. Combined use of LiCl with isopropanol as precipitating agents (optimal ratio 2.5 M and 40%, respectively) significantly eliminates negative effects of using this salt (inhibition of reverse transcription and low RNA yield).
AB - Contamination of the sample with DNA is a problem when isolating RNA by phenol-chloroform extraction, and DNase treatment introduces an additional error in the analysis of gene expression. Bearing in mind the ability of LiCl to precipitate RNA selectively, we studied the possibility and advantage of using LiCl as a precipitation agent in the protocol for RNA precipitation [3] from frozen segments of large vessels. Combined use of LiCl with isopropanol as precipitating agents (optimal ratio 2.5 M and 40%, respectively) significantly eliminates negative effects of using this salt (inhibition of reverse transcription and low RNA yield).
KW - isopropanol
KW - lithium chloride
KW - precipitation selectivity
KW - reverse transcription
KW - RNA isolation
KW - RNA/genetics
KW - Phenol
KW - DNA
KW - Lithium
KW - Lithium Chloride/pharmacology
UR - http://www.scopus.com/inward/record.url?scp=85134515587&partnerID=8YFLogxK
U2 - 10.1007/s10517-022-05554-8
DO - 10.1007/s10517-022-05554-8
M3 - Article
C2 - 35852690
AN - SCOPUS:85134515587
VL - 173
SP - 384
EP - 389
JO - Bulletin of Experimental Biology and Medicine
JF - Bulletin of Experimental Biology and Medicine
SN - 0007-4888
IS - 3
ER -
ID: 36746481