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Evaluation of Advantages of Lithium Chloride as a Precipitating Agent in RNA Isolation from Frozen Vein Segments. / Korolenya, V. A.; Gavrilov, K. A.; Sevost’ianova, K. S. et al.

In: Bulletin of Experimental Biology and Medicine, Vol. 173, No. 3, 07.2022, p. 384-389.

Research output: Contribution to journal › Article › peer-review

Harvard

Korolenya, VA, Gavrilov, KA, Sevost’ianova, KS, Shevela, AI, Filipenko, ML & Smetanina, MA 2022, 'Evaluation of Advantages of Lithium Chloride as a Precipitating Agent in RNA Isolation from Frozen Vein Segments', Bulletin of Experimental Biology and Medicine, vol. 173, no. 3, pp. 384-389. https://doi.org/10.1007/s10517-022-05554-8

APA

Korolenya, V. A., Gavrilov, K. A., Sevost’ianova, K. S., Shevela, A. I., Filipenko, M. L., & Smetanina, M. A. (2022). Evaluation of Advantages of Lithium Chloride as a Precipitating Agent in RNA Isolation from Frozen Vein Segments. Bulletin of Experimental Biology and Medicine, 173(3), 384-389. https://doi.org/10.1007/s10517-022-05554-8

Vancouver

Korolenya VA, Gavrilov KA, Sevost’ianova KS, Shevela AI, Filipenko ML, Smetanina MA. Evaluation of Advantages of Lithium Chloride as a Precipitating Agent in RNA Isolation from Frozen Vein Segments. Bulletin of Experimental Biology and Medicine. 2022 Jul;173(3):384-389. doi: 10.1007/s10517-022-05554-8

Author

Korolenya, V. A. ; Gavrilov, K. A. ; Sevost’ianova, K. S. et al. / Evaluation of Advantages of Lithium Chloride as a Precipitating Agent in RNA Isolation from Frozen Vein Segments. In: Bulletin of Experimental Biology and Medicine. 2022 ; Vol. 173, No. 3. pp. 384-389.

BibTeX

@article{9738978516f046b9b0a1d62005cb05c3,
title = "Evaluation of Advantages of Lithium Chloride as a Precipitating Agent in RNA Isolation from Frozen Vein Segments",
abstract = "Contamination of the sample with DNA is a problem when isolating RNA by phenol-chloroform extraction, and DNase treatment introduces an additional error in the analysis of gene expression. Bearing in mind the ability of LiCl to precipitate RNA selectively, we studied the possibility and advantage of using LiCl as a precipitation agent in the protocol for RNA precipitation [3] from frozen segments of large vessels. Combined use of LiCl with isopropanol as precipitating agents (optimal ratio 2.5 M and 40%, respectively) significantly eliminates negative effects of using this salt (inhibition of reverse transcription and low RNA yield).",
keywords = "isopropanol, lithium chloride, precipitation selectivity, reverse transcription, RNA isolation, RNA/genetics, Phenol, DNA, Lithium, Lithium Chloride/pharmacology",
author = "Korolenya, {V. A.} and Gavrilov, {K. A.} and Sevost{\textquoteright}ianova, {K. S.} and Shevela, {A. I.} and Filipenko, {M. L.} and Smetanina, {M. A.}",
note = "Publisher Copyright: {\textcopyright} 2022, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
month = jul,
doi = "10.1007/s10517-022-05554-8",
language = "English",
volume = "173",
pages = "384--389",
journal = "Bulletin of Experimental Biology and Medicine",
issn = "0007-4888",
publisher = "Springer New York",
number = "3",

}

RIS

TY - JOUR

T1 - Evaluation of Advantages of Lithium Chloride as a Precipitating Agent in RNA Isolation from Frozen Vein Segments

AU - Korolenya, V. A.

AU - Gavrilov, K. A.

AU - Sevost’ianova, K. S.

AU - Shevela, A. I.

AU - Filipenko, M. L.

AU - Smetanina, M. A.

N1 - Publisher Copyright: © 2022, Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022/7

Y1 - 2022/7

N2 - Contamination of the sample with DNA is a problem when isolating RNA by phenol-chloroform extraction, and DNase treatment introduces an additional error in the analysis of gene expression. Bearing in mind the ability of LiCl to precipitate RNA selectively, we studied the possibility and advantage of using LiCl as a precipitation agent in the protocol for RNA precipitation [3] from frozen segments of large vessels. Combined use of LiCl with isopropanol as precipitating agents (optimal ratio 2.5 M and 40%, respectively) significantly eliminates negative effects of using this salt (inhibition of reverse transcription and low RNA yield).

AB - Contamination of the sample with DNA is a problem when isolating RNA by phenol-chloroform extraction, and DNase treatment introduces an additional error in the analysis of gene expression. Bearing in mind the ability of LiCl to precipitate RNA selectively, we studied the possibility and advantage of using LiCl as a precipitation agent in the protocol for RNA precipitation [3] from frozen segments of large vessels. Combined use of LiCl with isopropanol as precipitating agents (optimal ratio 2.5 M and 40%, respectively) significantly eliminates negative effects of using this salt (inhibition of reverse transcription and low RNA yield).

KW - isopropanol

KW - lithium chloride

KW - precipitation selectivity

KW - reverse transcription

KW - RNA isolation

KW - RNA/genetics

KW - Phenol

KW - DNA

KW - Lithium

KW - Lithium Chloride/pharmacology

UR - http://www.scopus.com/inward/record.url?scp=85134515587&partnerID=8YFLogxK

U2 - 10.1007/s10517-022-05554-8

DO - 10.1007/s10517-022-05554-8

M3 - Article

C2 - 35852690

AN - SCOPUS:85134515587

VL - 173

SP - 384

EP - 389

JO - Bulletin of Experimental Biology and Medicine

JF - Bulletin of Experimental Biology and Medicine

SN - 0007-4888

IS - 3

ER -

ID: 36746481