TY - JOUR

T1 - Effects of Three-time Administration of a Supramolecular Complex of Praziquantel with Disodium Glycyrrhizinate on Trematode Opisthorchis felineus in Hamsters

AU - Avgustinovich, Damira

AU - Lvova, Maria

AU - Vishnivetskaya, Galina

AU - Tsyganov, Mikhail

AU - Orlovskaya, Irina

AU - Toporkova, Lyudmila

AU - Goiman, Elena

AU - Dushkin, Aleksander

AU - Lyakhov, Nikolay

AU - Mordvinov, Viatcheslav

N1 - Funding Information: The study was supported by the Russian Foundation for Basic Research (grant No. 20-04-00139) and a budget project of ICG SB RAS (grant No. 0324-2019-0041) as well as was implemented using the equipment of the Center for Genetic Resources of Laboratory Animals (ICG SB RAS) supported by the Ministry of Science and Higher Education of Russia (unique identifier of the project: RFMEFI62119X0023) and within the framework of a state assignment for ISSCM SB RAS (project No. 0301-2019-0005). The English language was corrected and certified by shevchuk-editing.com. (Language Certificate of December 7, 2020). Publisher Copyright: © 2021, Witold Stefanski Institute of Parasitology, Polish Academy of Sciences. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/6

Y1 - 2021/6

N2 - Background: Praziquantel (PZQ) is the most commonly used anthelmintic drug for treating trematodiases. It was shown here that PZQ in complex with disodium glycyrrhizinate (PZQ-Na2GA, in the 1:10 ratio) has higher bioavailability than PZQ alone. Our aim was to determine the effects of three-time administration of PZQ-Na2GA in an experimental opisthorchiasis felinea model. Methods: The PZQ-Na2GA complex (1:10) at a 400 mg/kg dose (meaning 36.4 mg/kg PZQ) was administered to Opisthorchis felineus-infected hamsters three times under a “9:00 am–6:00 pm–9:00 am” regimen (PZQ-Na2GA × 3). Effects of treatment were assessed as a reduction of helminth load in the hamsters and as changes in physiological, hematological, and blood biochemical parameters. The helminths extracted from the liver of the hamsters that received PZQ-Na2GA thrice were assayed for sensitivity to PZQ in vitro. Results: PZQ-Na2GA × 3 reduced the number of O. felineus helminths in the liver by 87%, which is 30% better than a previously reported effect of one-time administration of the complex. Meanwhile, relative weights of the liver and thymus diminished, and some hematological parameters improved. The helminths extracted from the hamsters 1 month after the PZQ-Na2GA × 3 treatment showed elevated sensitivity to PZQ, as determined in vitro. Conclusion: Compared with previously published data on the effectiveness of various drugs in experimental opisthorchiasis felinea, PZQ-Na2GA × 3 exerts the most potent anthelmintic effect. In addition, PZQ-Na2GA × 3 improves physiological status of O. felineus-infected hamsters and sensitizes the surviving parasites to subsequent PZQ treatment.

AB - Background: Praziquantel (PZQ) is the most commonly used anthelmintic drug for treating trematodiases. It was shown here that PZQ in complex with disodium glycyrrhizinate (PZQ-Na2GA, in the 1:10 ratio) has higher bioavailability than PZQ alone. Our aim was to determine the effects of three-time administration of PZQ-Na2GA in an experimental opisthorchiasis felinea model. Methods: The PZQ-Na2GA complex (1:10) at a 400 mg/kg dose (meaning 36.4 mg/kg PZQ) was administered to Opisthorchis felineus-infected hamsters three times under a “9:00 am–6:00 pm–9:00 am” regimen (PZQ-Na2GA × 3). Effects of treatment were assessed as a reduction of helminth load in the hamsters and as changes in physiological, hematological, and blood biochemical parameters. The helminths extracted from the liver of the hamsters that received PZQ-Na2GA thrice were assayed for sensitivity to PZQ in vitro. Results: PZQ-Na2GA × 3 reduced the number of O. felineus helminths in the liver by 87%, which is 30% better than a previously reported effect of one-time administration of the complex. Meanwhile, relative weights of the liver and thymus diminished, and some hematological parameters improved. The helminths extracted from the hamsters 1 month after the PZQ-Na2GA × 3 treatment showed elevated sensitivity to PZQ, as determined in vitro. Conclusion: Compared with previously published data on the effectiveness of various drugs in experimental opisthorchiasis felinea, PZQ-Na2GA × 3 exerts the most potent anthelmintic effect. In addition, PZQ-Na2GA × 3 improves physiological status of O. felineus-infected hamsters and sensitizes the surviving parasites to subsequent PZQ treatment.

KW - Disodium glycyrrhizinate

KW - Hamsters

KW - In vitro

KW - Opisthorchis felineus

KW - Praziquantel

KW - FIBROSIS

KW - VIVERRINI

KW - ACID

KW - INFECTION

KW - EXPRESSION

KW - CLONORCHIASIS

UR - http://www.scopus.com/inward/record.url?scp=85099179577&partnerID=8YFLogxK

U2 - 10.1007/s11686-020-00329-5

DO - 10.1007/s11686-020-00329-5

M3 - Article

C2 - 33420597

AN - SCOPUS:85099179577

VL - 66

SP - 623

EP - 630

JO - Acta Parasitologica

JF - Acta Parasitologica

SN - 1230-2821

IS - 2

ER -