Research output: Contribution to journal › Article › peer-review
Effects of Three-time Administration of a Supramolecular Complex of Praziquantel with Disodium Glycyrrhizinate on Trematode Opisthorchis felineus in Hamsters. / Avgustinovich, Damira; Lvova, Maria; Vishnivetskaya, Galina et al.
In: Acta Parasitologica, Vol. 66, No. 2, 06.2021, p. 623-630.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Effects of Three-time Administration of a Supramolecular Complex of Praziquantel with Disodium Glycyrrhizinate on Trematode Opisthorchis felineus in Hamsters
AU - Avgustinovich, Damira
AU - Lvova, Maria
AU - Vishnivetskaya, Galina
AU - Tsyganov, Mikhail
AU - Orlovskaya, Irina
AU - Toporkova, Lyudmila
AU - Goiman, Elena
AU - Dushkin, Aleksander
AU - Lyakhov, Nikolay
AU - Mordvinov, Viatcheslav
N1 - Funding Information: The study was supported by the Russian Foundation for Basic Research (grant No. 20-04-00139) and a budget project of ICG SB RAS (grant No. 0324-2019-0041) as well as was implemented using the equipment of the Center for Genetic Resources of Laboratory Animals (ICG SB RAS) supported by the Ministry of Science and Higher Education of Russia (unique identifier of the project: RFMEFI62119X0023) and within the framework of a state assignment for ISSCM SB RAS (project No. 0301-2019-0005). The English language was corrected and certified by shevchuk-editing.com. (Language Certificate of December 7, 2020). Publisher Copyright: © 2021, Witold Stefanski Institute of Parasitology, Polish Academy of Sciences. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/6
Y1 - 2021/6
N2 - Background: Praziquantel (PZQ) is the most commonly used anthelmintic drug for treating trematodiases. It was shown here that PZQ in complex with disodium glycyrrhizinate (PZQ-Na2GA, in the 1:10 ratio) has higher bioavailability than PZQ alone. Our aim was to determine the effects of three-time administration of PZQ-Na2GA in an experimental opisthorchiasis felinea model. Methods: The PZQ-Na2GA complex (1:10) at a 400 mg/kg dose (meaning 36.4 mg/kg PZQ) was administered to Opisthorchis felineus-infected hamsters three times under a “9:00 am–6:00 pm–9:00 am” regimen (PZQ-Na2GA × 3). Effects of treatment were assessed as a reduction of helminth load in the hamsters and as changes in physiological, hematological, and blood biochemical parameters. The helminths extracted from the liver of the hamsters that received PZQ-Na2GA thrice were assayed for sensitivity to PZQ in vitro. Results: PZQ-Na2GA × 3 reduced the number of O. felineus helminths in the liver by 87%, which is 30% better than a previously reported effect of one-time administration of the complex. Meanwhile, relative weights of the liver and thymus diminished, and some hematological parameters improved. The helminths extracted from the hamsters 1 month after the PZQ-Na2GA × 3 treatment showed elevated sensitivity to PZQ, as determined in vitro. Conclusion: Compared with previously published data on the effectiveness of various drugs in experimental opisthorchiasis felinea, PZQ-Na2GA × 3 exerts the most potent anthelmintic effect. In addition, PZQ-Na2GA × 3 improves physiological status of O. felineus-infected hamsters and sensitizes the surviving parasites to subsequent PZQ treatment.
AB - Background: Praziquantel (PZQ) is the most commonly used anthelmintic drug for treating trematodiases. It was shown here that PZQ in complex with disodium glycyrrhizinate (PZQ-Na2GA, in the 1:10 ratio) has higher bioavailability than PZQ alone. Our aim was to determine the effects of three-time administration of PZQ-Na2GA in an experimental opisthorchiasis felinea model. Methods: The PZQ-Na2GA complex (1:10) at a 400 mg/kg dose (meaning 36.4 mg/kg PZQ) was administered to Opisthorchis felineus-infected hamsters three times under a “9:00 am–6:00 pm–9:00 am” regimen (PZQ-Na2GA × 3). Effects of treatment were assessed as a reduction of helminth load in the hamsters and as changes in physiological, hematological, and blood biochemical parameters. The helminths extracted from the liver of the hamsters that received PZQ-Na2GA thrice were assayed for sensitivity to PZQ in vitro. Results: PZQ-Na2GA × 3 reduced the number of O. felineus helminths in the liver by 87%, which is 30% better than a previously reported effect of one-time administration of the complex. Meanwhile, relative weights of the liver and thymus diminished, and some hematological parameters improved. The helminths extracted from the hamsters 1 month after the PZQ-Na2GA × 3 treatment showed elevated sensitivity to PZQ, as determined in vitro. Conclusion: Compared with previously published data on the effectiveness of various drugs in experimental opisthorchiasis felinea, PZQ-Na2GA × 3 exerts the most potent anthelmintic effect. In addition, PZQ-Na2GA × 3 improves physiological status of O. felineus-infected hamsters and sensitizes the surviving parasites to subsequent PZQ treatment.
KW - Disodium glycyrrhizinate
KW - Hamsters
KW - In vitro
KW - Opisthorchis felineus
KW - Praziquantel
KW - FIBROSIS
KW - VIVERRINI
KW - ACID
KW - INFECTION
KW - EXPRESSION
KW - CLONORCHIASIS
UR - http://www.scopus.com/inward/record.url?scp=85099179577&partnerID=8YFLogxK
U2 - 10.1007/s11686-020-00329-5
DO - 10.1007/s11686-020-00329-5
M3 - Article
C2 - 33420597
AN - SCOPUS:85099179577
VL - 66
SP - 623
EP - 630
JO - Acta Parasitologica
JF - Acta Parasitologica
SN - 1230-2821
IS - 2
ER -
ID: 27436193