TY - JOUR

T1 - Effects of stearic acid on the embryo cryopreservation in mouse

AU - Igonina, T N

AU - Rakhmanova, T A

AU - Omelchenko, A N

AU - Okotrub, K A

AU - Brusentsev, E Yu

AU - Rozhkova, I N

AU - Amstislavsky, S Ya

N1 - Публикация для корректировки.

PY - 2024

Y1 - 2024

N2 - BACKGROUND: Intracellular lipids are sensitive to freezing. Lipidome modification is an important tool for studying the role of intracellular lipids in cryotolerance of mammalian oocytes and preimplantation embryos.OBJECTIVE: To study the effects of in vitro exposure of murine embryos to saturated stearic acid (SA) on the lipid content, embryo development and cryotolerance.MATERIALS AND METHODS: In vivo derived mouse embryos were cultured with 100 uM SA for 48 h up to the morula/blastocyst stage. Some of the SA-treated embryos were chosen for the evaluation of their development competence and the change in the lipidome, and other embryos were either slowly frozen or rapidly vitrified.RESULTS: Nile red staining combined with confocal laser scanning microscopy revealed a decrease in the total amount of lipids in the SA-treated embryos. Raman measurements showed that the lipid unsaturation was lower in embryos after in vitro SA culture. The addition of SA did not affect the embryo development before cryopreservation, but negatively affected the results of slow freezing cryopreservation and vitrification.CONCLUSION: In vitro SA exposure lowered the total amount of intracellular lipids and unsaturation in mouse embryos. The changes were accompanied with a significantly lower efficacy of embryo cryopreservation. https://doi.org/10.54680/fr24110110512.

AB - BACKGROUND: Intracellular lipids are sensitive to freezing. Lipidome modification is an important tool for studying the role of intracellular lipids in cryotolerance of mammalian oocytes and preimplantation embryos.OBJECTIVE: To study the effects of in vitro exposure of murine embryos to saturated stearic acid (SA) on the lipid content, embryo development and cryotolerance.MATERIALS AND METHODS: In vivo derived mouse embryos were cultured with 100 uM SA for 48 h up to the morula/blastocyst stage. Some of the SA-treated embryos were chosen for the evaluation of their development competence and the change in the lipidome, and other embryos were either slowly frozen or rapidly vitrified.RESULTS: Nile red staining combined with confocal laser scanning microscopy revealed a decrease in the total amount of lipids in the SA-treated embryos. Raman measurements showed that the lipid unsaturation was lower in embryos after in vitro SA culture. The addition of SA did not affect the embryo development before cryopreservation, but negatively affected the results of slow freezing cryopreservation and vitrification.CONCLUSION: In vitro SA exposure lowered the total amount of intracellular lipids and unsaturation in mouse embryos. The changes were accompanied with a significantly lower efficacy of embryo cryopreservation. https://doi.org/10.54680/fr24110110512.

KW - Animals

KW - Mice

KW - Cryopreservation/methods

KW - Vitrification

KW - Embryo, Mammalian

KW - Blastocyst

KW - Embryonic Development

KW - Lipids

KW - Embryo Culture Techniques

KW - Mammals

KW - Stearic Acids

KW - lipids

KW - stearic acid

KW - embryo cryopreservation

KW - Raman spectroscopy

KW - confocal microscopy

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85189278771&origin=inward&txGid=d2cf00c433bafb47c817065ca19aed2e

UR - https://www.mendeley.com/catalogue/65f73fa7-161b-36aa-a6f0-0167462c883e/

U2 - 10.54680/fr24110110512

DO - 10.54680/fr24110110512

M3 - Article

C2 - 38538369

VL - 45

SP - 28

EP - 35

JO - Cryo letters

JF - Cryo letters

SN - 0143-2044

IS - 1

ER -