Research output: Contribution to journal › Article › peer-review
Effects of stearic acid on the embryo cryopreservation in mouse. / Igonina, T N; Rakhmanova, T A; Omelchenko, A N et al.
In: Cryo letters, Vol. 45, No. 1, 2024, p. 28-35.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Effects of stearic acid on the embryo cryopreservation in mouse
AU - Igonina, T N
AU - Rakhmanova, T A
AU - Omelchenko, A N
AU - Okotrub, K A
AU - Brusentsev, E Yu
AU - Rozhkova, I N
AU - Amstislavsky, S Ya
N1 - Публикация для корректировки.
PY - 2024
Y1 - 2024
N2 - BACKGROUND: Intracellular lipids are sensitive to freezing. Lipidome modification is an important tool for studying the role of intracellular lipids in cryotolerance of mammalian oocytes and preimplantation embryos.OBJECTIVE: To study the effects of in vitro exposure of murine embryos to saturated stearic acid (SA) on the lipid content, embryo development and cryotolerance.MATERIALS AND METHODS: In vivo derived mouse embryos were cultured with 100 uM SA for 48 h up to the morula/blastocyst stage. Some of the SA-treated embryos were chosen for the evaluation of their development competence and the change in the lipidome, and other embryos were either slowly frozen or rapidly vitrified.RESULTS: Nile red staining combined with confocal laser scanning microscopy revealed a decrease in the total amount of lipids in the SA-treated embryos. Raman measurements showed that the lipid unsaturation was lower in embryos after in vitro SA culture. The addition of SA did not affect the embryo development before cryopreservation, but negatively affected the results of slow freezing cryopreservation and vitrification.CONCLUSION: In vitro SA exposure lowered the total amount of intracellular lipids and unsaturation in mouse embryos. The changes were accompanied with a significantly lower efficacy of embryo cryopreservation. https://doi.org/10.54680/fr24110110512.
AB - BACKGROUND: Intracellular lipids are sensitive to freezing. Lipidome modification is an important tool for studying the role of intracellular lipids in cryotolerance of mammalian oocytes and preimplantation embryos.OBJECTIVE: To study the effects of in vitro exposure of murine embryos to saturated stearic acid (SA) on the lipid content, embryo development and cryotolerance.MATERIALS AND METHODS: In vivo derived mouse embryos were cultured with 100 uM SA for 48 h up to the morula/blastocyst stage. Some of the SA-treated embryos were chosen for the evaluation of their development competence and the change in the lipidome, and other embryos were either slowly frozen or rapidly vitrified.RESULTS: Nile red staining combined with confocal laser scanning microscopy revealed a decrease in the total amount of lipids in the SA-treated embryos. Raman measurements showed that the lipid unsaturation was lower in embryos after in vitro SA culture. The addition of SA did not affect the embryo development before cryopreservation, but negatively affected the results of slow freezing cryopreservation and vitrification.CONCLUSION: In vitro SA exposure lowered the total amount of intracellular lipids and unsaturation in mouse embryos. The changes were accompanied with a significantly lower efficacy of embryo cryopreservation. https://doi.org/10.54680/fr24110110512.
KW - Animals
KW - Mice
KW - Cryopreservation/methods
KW - Vitrification
KW - Embryo, Mammalian
KW - Blastocyst
KW - Embryonic Development
KW - Lipids
KW - Embryo Culture Techniques
KW - Mammals
KW - Stearic Acids
KW - lipids
KW - stearic acid
KW - embryo cryopreservation
KW - Raman spectroscopy
KW - confocal microscopy
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85189278771&origin=inward&txGid=d2cf00c433bafb47c817065ca19aed2e
UR - https://www.mendeley.com/catalogue/65f73fa7-161b-36aa-a6f0-0167462c883e/
U2 - 10.54680/fr24110110512
DO - 10.54680/fr24110110512
M3 - Article
C2 - 38538369
VL - 45
SP - 28
EP - 35
JO - Cryo letters
JF - Cryo letters
SN - 0143-2044
IS - 1
ER -
ID: 60482299