Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Effects of forskolin on cryopreservation and embryo development in the domestic cat. / Rakhmanova, Tamara; Mokrousova, Valentina; Okotrub, Svetlana и др.
в: Theriogenology, Том 210, 15.10.2023, стр. 192-198.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Effects of forskolin on cryopreservation and embryo development in the domestic cat
AU - Rakhmanova, Tamara
AU - Mokrousova, Valentina
AU - Okotrub, Svetlana
AU - Kizilova, Elena
AU - Brusentsev, Eugeny
AU - Amstislavsky, Sergei
N1 - Fundings: This work was supported by the budget Project of the Institute of Cytology and Genetics Siberian Branch of the Russian Academy of Sciences [grant number FWNR-2022-0011] and using the equipment of the Center for Genetic Resources of Laboratory Animals at ICG Siberian Branch of the Russian Academy of Sciences, supported by the Ministry of Education and Science of Russia [Unique identifier of the project RFMEFI62117X0023]. The microscopical work was performed using the facilities and equipment of the Microscopic Center of ICG Siberian Branch of the Russian Academy of Sciences. Copyright © 2023 Elsevier Inc. All rights reserved.
PY - 2023/10/15
Y1 - 2023/10/15
N2 - One of the approaches to improve cryotolerance in lipid-rich embryos is to modify their lipidome in vitro. This work is aimed to study the effects of forskolin exposure on the in vitro embryo development of the domestic cat and to evaluate how the change in lipid content affects the cryopreservation results. In vitro-derived embryos were cultured with 10 μM forskolin from the 2-cell stage for 24 h or 96/168 h to the morula/blastocyst stage. Some of the embryos treated with forskolin for 24 h were cryopreserved with slow freezing, the other ones were used to characterize their developmental rates and the amount of intracellular lipids. The in vitro exposure to forskolin had a positive effect on the embryo development, as more embryos developed to the morula stage in the forskolin-treated group (92.9%) compared to the controls (64.7%) after 120 h of in vitro culture (IVC). Nile Red staining revealed a reduced amount of intracellular lipids in the forskolin-treated embryos. The percentage of embryos developed to the morula stage was lower in the frozen-thawed embryos not treated with forskolin (54.5%), but not in the frozen-thawed forskolin-treated group (63.6%) as compared to non-frozen controls (80.8%). Thus, the exposure of embryos to forskolin in vitro reduced the level of intracellular lipids and affected embryo development before and after cryopreservation.
AB - One of the approaches to improve cryotolerance in lipid-rich embryos is to modify their lipidome in vitro. This work is aimed to study the effects of forskolin exposure on the in vitro embryo development of the domestic cat and to evaluate how the change in lipid content affects the cryopreservation results. In vitro-derived embryos were cultured with 10 μM forskolin from the 2-cell stage for 24 h or 96/168 h to the morula/blastocyst stage. Some of the embryos treated with forskolin for 24 h were cryopreserved with slow freezing, the other ones were used to characterize their developmental rates and the amount of intracellular lipids. The in vitro exposure to forskolin had a positive effect on the embryo development, as more embryos developed to the morula stage in the forskolin-treated group (92.9%) compared to the controls (64.7%) after 120 h of in vitro culture (IVC). Nile Red staining revealed a reduced amount of intracellular lipids in the forskolin-treated embryos. The percentage of embryos developed to the morula stage was lower in the frozen-thawed embryos not treated with forskolin (54.5%), but not in the frozen-thawed forskolin-treated group (63.6%) as compared to non-frozen controls (80.8%). Thus, the exposure of embryos to forskolin in vitro reduced the level of intracellular lipids and affected embryo development before and after cryopreservation.
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85166046426&origin=inward&txGid=aefc81c6c9841bc9e6b9df9b0cb18d6e
UR - https://www.mendeley.com/catalogue/ccd6a769-a4cf-3352-9f4e-c2c499cc4140/
U2 - 10.1016/j.theriogenology.2023.07.035
DO - 10.1016/j.theriogenology.2023.07.035
M3 - Article
C2 - 37523940
VL - 210
SP - 192
EP - 198
JO - Theriogenology
JF - Theriogenology
SN - 0093-691X
ER -
ID: 53439995