TY - JOUR

T1 - Effects of forskolin on cryopreservation and embryo development in the domestic cat

AU - Rakhmanova, Tamara

AU - Mokrousova, Valentina

AU - Okotrub, Svetlana

AU - Kizilova, Elena

AU - Brusentsev, Eugeny

AU - Amstislavsky, Sergei

N1 - Fundings: This work was supported by the budget Project of the Institute of Cytology and Genetics Siberian Branch of the Russian Academy of Sciences [grant number FWNR-2022-0011] and using the equipment of the Center for Genetic Resources of Laboratory Animals at ICG Siberian Branch of the Russian Academy of Sciences, supported by the Ministry of Education and Science of Russia [Unique identifier of the project RFMEFI62117X0023]. The microscopical work was performed using the facilities and equipment of the Microscopic Center of ICG Siberian Branch of the Russian Academy of Sciences. Copyright © 2023 Elsevier Inc. All rights reserved.

PY - 2023/10/15

Y1 - 2023/10/15

N2 - One of the approaches to improve cryotolerance in lipid-rich embryos is to modify their lipidome in vitro. This work is aimed to study the effects of forskolin exposure on the in vitro embryo development of the domestic cat and to evaluate how the change in lipid content affects the cryopreservation results. In vitro-derived embryos were cultured with 10 μM forskolin from the 2-cell stage for 24 h or 96/168 h to the morula/blastocyst stage. Some of the embryos treated with forskolin for 24 h were cryopreserved with slow freezing, the other ones were used to characterize their developmental rates and the amount of intracellular lipids. The in vitro exposure to forskolin had a positive effect on the embryo development, as more embryos developed to the morula stage in the forskolin-treated group (92.9%) compared to the controls (64.7%) after 120 h of in vitro culture (IVC). Nile Red staining revealed a reduced amount of intracellular lipids in the forskolin-treated embryos. The percentage of embryos developed to the morula stage was lower in the frozen-thawed embryos not treated with forskolin (54.5%), but not in the frozen-thawed forskolin-treated group (63.6%) as compared to non-frozen controls (80.8%). Thus, the exposure of embryos to forskolin in vitro reduced the level of intracellular lipids and affected embryo development before and after cryopreservation.

AB - One of the approaches to improve cryotolerance in lipid-rich embryos is to modify their lipidome in vitro. This work is aimed to study the effects of forskolin exposure on the in vitro embryo development of the domestic cat and to evaluate how the change in lipid content affects the cryopreservation results. In vitro-derived embryos were cultured with 10 μM forskolin from the 2-cell stage for 24 h or 96/168 h to the morula/blastocyst stage. Some of the embryos treated with forskolin for 24 h were cryopreserved with slow freezing, the other ones were used to characterize their developmental rates and the amount of intracellular lipids. The in vitro exposure to forskolin had a positive effect on the embryo development, as more embryos developed to the morula stage in the forskolin-treated group (92.9%) compared to the controls (64.7%) after 120 h of in vitro culture (IVC). Nile Red staining revealed a reduced amount of intracellular lipids in the forskolin-treated embryos. The percentage of embryos developed to the morula stage was lower in the frozen-thawed embryos not treated with forskolin (54.5%), but not in the frozen-thawed forskolin-treated group (63.6%) as compared to non-frozen controls (80.8%). Thus, the exposure of embryos to forskolin in vitro reduced the level of intracellular lipids and affected embryo development before and after cryopreservation.

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85166046426&origin=inward&txGid=aefc81c6c9841bc9e6b9df9b0cb18d6e

UR - https://www.mendeley.com/catalogue/ccd6a769-a4cf-3352-9f4e-c2c499cc4140/

U2 - 10.1016/j.theriogenology.2023.07.035

DO - 10.1016/j.theriogenology.2023.07.035

M3 - Article

C2 - 37523940

VL - 210

SP - 192

EP - 198

JO - Theriogenology

JF - Theriogenology

SN - 0093-691X

ER -