TY - JOUR

T1 - Effect of cryopreservation on Odc1 and RhoA genes expression in diapausing mouse blastocysts

AU - Amstislavsky, Sergei

AU - Brusentsev, Eugeny

AU - Lebedeva, Daria

AU - Rozhkova, Irina

AU - Rakhmanova, Tamara

AU - Okotrub, Svetlana

AU - Kozeneva, Varvara

AU - Igonina, Tatyana

AU - Babochkina, Tatyana

N1 - Russian Science Foundation, Grant/AwardNumber: 23-24- 00313.

PY - 2024/5

Y1 - 2024/5

N2 - The possibility of embryo cryopreservation is important for applying the genome resource banking (GRB) concept to those mammalian species that exhibit embryonal diapause in their early development. Odc1 encodes ODC1, which is a key enzyme in polyamine synthesis. RhoA is an essential part of Rho/ROCK system. Both Odc1 and RhoA play an important role in preimplantation embryo development. Studying these systems in mammalian species with obligate or experimentally designed embryonic diapause may provide insight into the molecular machinery underlying embryo dormancy and re-activation. The effect of cryopreservation procedures on the expression of the Odc1 and RhoA in diapausing embryos has not been properly studied yet. The purpose of this work is to address the possibility of cryopreservation diapausing embryos and to estimate the expression of the Odc1 and RhoA genes in diapausing and non-diapausing embryos before and after freeze–thaw procedures using ovariectomized progesterone treated mice as a model. Both diapausing and non-diapausing in vivo-derived embryos continued their development in vitro after freezing–thawing as evidenced by blastocoel re-expansion. Although cryopreservation dramatically decreased the expression of the Odc1 and RhoA genes in non-diapausing embryos, no such effects have been observed in diapausing embryos where these genes were already at the low level before freeze–thaw procedures. Future studies may attempt to facilitate the re-activation of diapausing embryos, for example frozen–thawed ones, specifically targeting Odc1 or Rho/ROCK system.

AB - The possibility of embryo cryopreservation is important for applying the genome resource banking (GRB) concept to those mammalian species that exhibit embryonal diapause in their early development. Odc1 encodes ODC1, which is a key enzyme in polyamine synthesis. RhoA is an essential part of Rho/ROCK system. Both Odc1 and RhoA play an important role in preimplantation embryo development. Studying these systems in mammalian species with obligate or experimentally designed embryonic diapause may provide insight into the molecular machinery underlying embryo dormancy and re-activation. The effect of cryopreservation procedures on the expression of the Odc1 and RhoA in diapausing embryos has not been properly studied yet. The purpose of this work is to address the possibility of cryopreservation diapausing embryos and to estimate the expression of the Odc1 and RhoA genes in diapausing and non-diapausing embryos before and after freeze–thaw procedures using ovariectomized progesterone treated mice as a model. Both diapausing and non-diapausing in vivo-derived embryos continued their development in vitro after freezing–thawing as evidenced by blastocoel re-expansion. Although cryopreservation dramatically decreased the expression of the Odc1 and RhoA genes in non-diapausing embryos, no such effects have been observed in diapausing embryos where these genes were already at the low level before freeze–thaw procedures. Future studies may attempt to facilitate the re-activation of diapausing embryos, for example frozen–thawed ones, specifically targeting Odc1 or Rho/ROCK system.

KW - cryopreservation

KW - diapausing blastocysts

KW - genes expression

KW - mice

KW - rhoA GTP-Binding Protein/genetics

KW - Diapause

KW - Embryo Culture Techniques

KW - Embryonic Development

KW - Animals

KW - Blastocyst/metabolism

KW - Cryopreservation

KW - Gene Expression Regulation, Developmental

KW - Female

KW - Mice

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85192138757&origin=inward&txGid=49c27bbaef91c09075f6bb895976cf6a

UR - https://www.mendeley.com/catalogue/1a34a60b-92d5-38b8-bfec-e8846db1efc4/

U2 - 10.1111/rda.14576

DO - 10.1111/rda.14576

M3 - Article

C2 - 38712681

VL - 59

JO - Reproduction in Domestic Animals

JF - Reproduction in Domestic Animals

SN - 0936-6768

IS - 5

M1 - e14576

ER -