Research output: Contribution to journal › Article › peer-review
Effect of cryopreservation on Odc1 and RhoA genes expression in diapausing mouse blastocysts. / Amstislavsky, Sergei; Brusentsev, Eugeny; Lebedeva, Daria et al.
In: Reproduction in Domestic Animals, Vol. 59, No. 5, e14576, 05.2024.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Effect of cryopreservation on Odc1 and RhoA genes expression in diapausing mouse blastocysts
AU - Amstislavsky, Sergei
AU - Brusentsev, Eugeny
AU - Lebedeva, Daria
AU - Rozhkova, Irina
AU - Rakhmanova, Tamara
AU - Okotrub, Svetlana
AU - Kozeneva, Varvara
AU - Igonina, Tatyana
AU - Babochkina, Tatyana
N1 - Russian Science Foundation, Grant/AwardNumber: 23-24- 00313.
PY - 2024/5
Y1 - 2024/5
N2 - The possibility of embryo cryopreservation is important for applying the genome resource banking (GRB) concept to those mammalian species that exhibit embryonal diapause in their early development. Odc1 encodes ODC1, which is a key enzyme in polyamine synthesis. RhoA is an essential part of Rho/ROCK system. Both Odc1 and RhoA play an important role in preimplantation embryo development. Studying these systems in mammalian species with obligate or experimentally designed embryonic diapause may provide insight into the molecular machinery underlying embryo dormancy and re-activation. The effect of cryopreservation procedures on the expression of the Odc1 and RhoA in diapausing embryos has not been properly studied yet. The purpose of this work is to address the possibility of cryopreservation diapausing embryos and to estimate the expression of the Odc1 and RhoA genes in diapausing and non-diapausing embryos before and after freeze–thaw procedures using ovariectomized progesterone treated mice as a model. Both diapausing and non-diapausing in vivo-derived embryos continued their development in vitro after freezing–thawing as evidenced by blastocoel re-expansion. Although cryopreservation dramatically decreased the expression of the Odc1 and RhoA genes in non-diapausing embryos, no such effects have been observed in diapausing embryos where these genes were already at the low level before freeze–thaw procedures. Future studies may attempt to facilitate the re-activation of diapausing embryos, for example frozen–thawed ones, specifically targeting Odc1 or Rho/ROCK system.
AB - The possibility of embryo cryopreservation is important for applying the genome resource banking (GRB) concept to those mammalian species that exhibit embryonal diapause in their early development. Odc1 encodes ODC1, which is a key enzyme in polyamine synthesis. RhoA is an essential part of Rho/ROCK system. Both Odc1 and RhoA play an important role in preimplantation embryo development. Studying these systems in mammalian species with obligate or experimentally designed embryonic diapause may provide insight into the molecular machinery underlying embryo dormancy and re-activation. The effect of cryopreservation procedures on the expression of the Odc1 and RhoA in diapausing embryos has not been properly studied yet. The purpose of this work is to address the possibility of cryopreservation diapausing embryos and to estimate the expression of the Odc1 and RhoA genes in diapausing and non-diapausing embryos before and after freeze–thaw procedures using ovariectomized progesterone treated mice as a model. Both diapausing and non-diapausing in vivo-derived embryos continued their development in vitro after freezing–thawing as evidenced by blastocoel re-expansion. Although cryopreservation dramatically decreased the expression of the Odc1 and RhoA genes in non-diapausing embryos, no such effects have been observed in diapausing embryos where these genes were already at the low level before freeze–thaw procedures. Future studies may attempt to facilitate the re-activation of diapausing embryos, for example frozen–thawed ones, specifically targeting Odc1 or Rho/ROCK system.
KW - cryopreservation
KW - diapausing blastocysts
KW - genes expression
KW - mice
KW - rhoA GTP-Binding Protein/genetics
KW - Diapause
KW - Embryo Culture Techniques
KW - Embryonic Development
KW - Animals
KW - Blastocyst/metabolism
KW - Cryopreservation
KW - Gene Expression Regulation, Developmental
KW - Female
KW - Mice
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85192138757&origin=inward&txGid=49c27bbaef91c09075f6bb895976cf6a
UR - https://www.mendeley.com/catalogue/1a34a60b-92d5-38b8-bfec-e8846db1efc4/
U2 - 10.1111/rda.14576
DO - 10.1111/rda.14576
M3 - Article
C2 - 38712681
VL - 59
JO - Reproduction in Domestic Animals
JF - Reproduction in Domestic Animals
SN - 0936-6768
IS - 5
M1 - e14576
ER -
ID: 61042445