Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
DNA methylation and gene expression profiling reveal MFAP5 as a regulatory driver of extracellular matrix remodeling in varicose vein disease. / Smetanina, Mariya A.; Kel, Alexander E.; Sevost'Ianova, Ksenia S. и др.
в: Epigenomics, Том 10, № 8, 01.08.2018, стр. 1103-1119.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - DNA methylation and gene expression profiling reveal MFAP5 as a regulatory driver of extracellular matrix remodeling in varicose vein disease
AU - Smetanina, Mariya A.
AU - Kel, Alexander E.
AU - Sevost'Ianova, Ksenia S.
AU - Maiborodin, Igor V.
AU - Shevela, Andrey I.
AU - Zolotukhin, Igor A.
AU - Stegmaier, Philip
AU - Filipenko, Maxim L.
N1 - Publisher Copyright: © 2018 Future Medicine Ltd.
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Aim: To integrate transcriptomic and DNA-methylomic measurements on varicose versus normal veins using a systems biological analysis to shed light on the interplay between genetic and epigenetic factors. Materials & methods: Differential expression and methylation were measured using microarrays, supported by real-time quantitative PCR and immunohistochemistry confirmation for relevant gene products. A systems biological 'upstream analysis' was further applied. Results: We identified several potential key players contributing to extracellular matrix remodeling in varicose veins. Specifically, our analysis suggests MFAP5 acting as a master regulator, upstream of integrins, of the cellular network affecting the varicose vein condition. Possible mechanism and pathogenic model were outlined. Conclusion: A coherent model proposed incorporates the relevant signaling networks and will hopefully aid further studies on varicose vein pathogenesis.
AB - Aim: To integrate transcriptomic and DNA-methylomic measurements on varicose versus normal veins using a systems biological analysis to shed light on the interplay between genetic and epigenetic factors. Materials & methods: Differential expression and methylation were measured using microarrays, supported by real-time quantitative PCR and immunohistochemistry confirmation for relevant gene products. A systems biological 'upstream analysis' was further applied. Results: We identified several potential key players contributing to extracellular matrix remodeling in varicose veins. Specifically, our analysis suggests MFAP5 acting as a master regulator, upstream of integrins, of the cellular network affecting the varicose vein condition. Possible mechanism and pathogenic model were outlined. Conclusion: A coherent model proposed incorporates the relevant signaling networks and will hopefully aid further studies on varicose vein pathogenesis.
KW - DNA methylation
KW - extracellular matrix
KW - gene expression
KW - MFAP5
KW - systems biological analysis
KW - varicose vein(s)
KW - vascular remodeling
UR - http://www.scopus.com/inward/record.url?scp=85053031219&partnerID=8YFLogxK
U2 - 10.2217/epi-2018-0001
DO - 10.2217/epi-2018-0001
M3 - Article
C2 - 30070582
AN - SCOPUS:85053031219
VL - 10
SP - 1103
EP - 1119
JO - Epigenomics
JF - Epigenomics
SN - 1750-1911
IS - 8
ER -
ID: 16483390