DNA methylation and gene expression profiling reveal MFAP5 as a regulatory driver of extracellular matrix remodeling in varicose vein disease

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DNA methylation and gene expression profiling reveal MFAP5 as a regulatory driver of extracellular matrix remodeling in varicose vein disease. / Smetanina, Mariya A.; Kel, Alexander E.; Sevost'Ianova, Ksenia S. et al.

In: Epigenomics, Vol. 10, No. 8, 01.08.2018, p. 1103-1119.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{cc50b22bdb7b4275861a683831c051d2,

title = "DNA methylation and gene expression profiling reveal MFAP5 as a regulatory driver of extracellular matrix remodeling in varicose vein disease",

abstract = "Aim: To integrate transcriptomic and DNA-methylomic measurements on varicose versus normal veins using a systems biological analysis to shed light on the interplay between genetic and epigenetic factors. Materials & methods: Differential expression and methylation were measured using microarrays, supported by real-time quantitative PCR and immunohistochemistry confirmation for relevant gene products. A systems biological 'upstream analysis' was further applied. Results: We identified several potential key players contributing to extracellular matrix remodeling in varicose veins. Specifically, our analysis suggests MFAP5 acting as a master regulator, upstream of integrins, of the cellular network affecting the varicose vein condition. Possible mechanism and pathogenic model were outlined. Conclusion: A coherent model proposed incorporates the relevant signaling networks and will hopefully aid further studies on varicose vein pathogenesis.",

keywords = "DNA methylation, extracellular matrix, gene expression, MFAP5, systems biological analysis, varicose vein(s), vascular remodeling",

author = "Smetanina, {Mariya A.} and Kel, {Alexander E.} and Sevost'Ianova, {Ksenia S.} and Maiborodin, {Igor V.} and Shevela, {Andrey I.} and Zolotukhin, {Igor A.} and Philip Stegmaier and Filipenko, {Maxim L.}",

note = "Publisher Copyright: {\textcopyright} 2018 Future Medicine Ltd.",

year = "2018",

month = aug,

day = "1",

doi = "10.2217/epi-2018-0001",

language = "English",

volume = "10",

pages = "1103--1119",

journal = "Epigenomics",

issn = "1750-1911",

publisher = "Future Medicine Ltd.",

number = "8",

}

RIS

TY - JOUR

T1 - DNA methylation and gene expression profiling reveal MFAP5 as a regulatory driver of extracellular matrix remodeling in varicose vein disease

AU - Smetanina, Mariya A.

AU - Kel, Alexander E.

AU - Sevost'Ianova, Ksenia S.

AU - Maiborodin, Igor V.

AU - Shevela, Andrey I.

AU - Zolotukhin, Igor A.

AU - Stegmaier, Philip

AU - Filipenko, Maxim L.

PY - 2018/8/1

Y1 - 2018/8/1

N2 - Aim: To integrate transcriptomic and DNA-methylomic measurements on varicose versus normal veins using a systems biological analysis to shed light on the interplay between genetic and epigenetic factors. Materials & methods: Differential expression and methylation were measured using microarrays, supported by real-time quantitative PCR and immunohistochemistry confirmation for relevant gene products. A systems biological 'upstream analysis' was further applied. Results: We identified several potential key players contributing to extracellular matrix remodeling in varicose veins. Specifically, our analysis suggests MFAP5 acting as a master regulator, upstream of integrins, of the cellular network affecting the varicose vein condition. Possible mechanism and pathogenic model were outlined. Conclusion: A coherent model proposed incorporates the relevant signaling networks and will hopefully aid further studies on varicose vein pathogenesis.

AB - Aim: To integrate transcriptomic and DNA-methylomic measurements on varicose versus normal veins using a systems biological analysis to shed light on the interplay between genetic and epigenetic factors. Materials & methods: Differential expression and methylation were measured using microarrays, supported by real-time quantitative PCR and immunohistochemistry confirmation for relevant gene products. A systems biological 'upstream analysis' was further applied. Results: We identified several potential key players contributing to extracellular matrix remodeling in varicose veins. Specifically, our analysis suggests MFAP5 acting as a master regulator, upstream of integrins, of the cellular network affecting the varicose vein condition. Possible mechanism and pathogenic model were outlined. Conclusion: A coherent model proposed incorporates the relevant signaling networks and will hopefully aid further studies on varicose vein pathogenesis.

KW - DNA methylation

KW - extracellular matrix

KW - gene expression

KW - MFAP5

KW - systems biological analysis

KW - varicose vein(s)

KW - vascular remodeling

UR - http://www.scopus.com/inward/record.url?scp=85053031219&partnerID=8YFLogxK

U2 - 10.2217/epi-2018-0001

DO - 10.2217/epi-2018-0001

M3 - Article

C2 - 30070582

AN - SCOPUS:85053031219

VL - 10

SP - 1103

EP - 1119

JO - Epigenomics

JF - Epigenomics

SN - 1750-1911

IS - 8

ER -

ID: 16483390