Standard

Determination of the Thermodynamic Parameters of DNA Double Helix Unwinding with the Help of Mechanical Methods. / Kurus, Nina N.; Dultsev, Fedor N.

в: ACS Omega, Том 3, № 3, 31.03.2018, стр. 2793-2797.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Kurus, NN & Dultsev, FN 2018, 'Determination of the Thermodynamic Parameters of DNA Double Helix Unwinding with the Help of Mechanical Methods', ACS Omega, Том. 3, № 3, стр. 2793-2797. https://doi.org/10.1021/acsomega.7b01815

APA

Kurus, N. N., & Dultsev, F. N. (2018). Determination of the Thermodynamic Parameters of DNA Double Helix Unwinding with the Help of Mechanical Methods. ACS Omega, 3(3), 2793-2797. https://doi.org/10.1021/acsomega.7b01815

Vancouver

Kurus NN, Dultsev FN. Determination of the Thermodynamic Parameters of DNA Double Helix Unwinding with the Help of Mechanical Methods. ACS Omega. 2018 март 31;3(3):2793-2797. doi: 10.1021/acsomega.7b01815

Author

Kurus, Nina N. ; Dultsev, Fedor N. / Determination of the Thermodynamic Parameters of DNA Double Helix Unwinding with the Help of Mechanical Methods. в: ACS Omega. 2018 ; Том 3, № 3. стр. 2793-2797.

BibTeX

@article{4f547844eaf243e2b7a8e74fcba2f607,
title = "Determination of the Thermodynamic Parameters of DNA Double Helix Unwinding with the Help of Mechanical Methods",
abstract = "For the first time, rupture event scanning (REVS) procedure based on quartz crystal microbalance (QCM) and involving only mechanical action was used to determine the height of the energy barrier for dsDNA unwinding. Melting point was determined with the help of this procedure. To determine the thermodynamic parameters including enthalpy, DNA denaturation was represented as a unimolecular process. This allowed us to recover the energy profiles from the experimental data obtained by force measurements at different scanning times (reaction times) for different temperatures. The thus obtained results were compared with the data obtained with the help of another mechanical method, namely, atomic force microscopy. The mechanism of DNA unwinding in QCM-based experiments through the unzipping mode, as proposed by us in previous works, was confirmed. Thus, we demonstrated that REVS procedure may be used to assess the thermodynamic parameters of dsDNA unwinding.",
keywords = "ADHESION, BOND RUPTURE FORCES, DUPLEX, DYNAMIC FORCE SPECTROSCOPY, ENERGY LANDSCAPES, EVENTS, MICROSCOPY, MISMATCHES, STRANDS, STRENGTH",
author = "Kurus, {Nina N.} and Dultsev, {Fedor N.}",
year = "2018",
month = mar,
day = "31",
doi = "10.1021/acsomega.7b01815",
language = "English",
volume = "3",
pages = "2793--2797",
journal = "ACS Omega",
issn = "2470-1343",
publisher = "American Chemical Society",
number = "3",

}

RIS

TY - JOUR

T1 - Determination of the Thermodynamic Parameters of DNA Double Helix Unwinding with the Help of Mechanical Methods

AU - Kurus, Nina N.

AU - Dultsev, Fedor N.

PY - 2018/3/31

Y1 - 2018/3/31

N2 - For the first time, rupture event scanning (REVS) procedure based on quartz crystal microbalance (QCM) and involving only mechanical action was used to determine the height of the energy barrier for dsDNA unwinding. Melting point was determined with the help of this procedure. To determine the thermodynamic parameters including enthalpy, DNA denaturation was represented as a unimolecular process. This allowed us to recover the energy profiles from the experimental data obtained by force measurements at different scanning times (reaction times) for different temperatures. The thus obtained results were compared with the data obtained with the help of another mechanical method, namely, atomic force microscopy. The mechanism of DNA unwinding in QCM-based experiments through the unzipping mode, as proposed by us in previous works, was confirmed. Thus, we demonstrated that REVS procedure may be used to assess the thermodynamic parameters of dsDNA unwinding.

AB - For the first time, rupture event scanning (REVS) procedure based on quartz crystal microbalance (QCM) and involving only mechanical action was used to determine the height of the energy barrier for dsDNA unwinding. Melting point was determined with the help of this procedure. To determine the thermodynamic parameters including enthalpy, DNA denaturation was represented as a unimolecular process. This allowed us to recover the energy profiles from the experimental data obtained by force measurements at different scanning times (reaction times) for different temperatures. The thus obtained results were compared with the data obtained with the help of another mechanical method, namely, atomic force microscopy. The mechanism of DNA unwinding in QCM-based experiments through the unzipping mode, as proposed by us in previous works, was confirmed. Thus, we demonstrated that REVS procedure may be used to assess the thermodynamic parameters of dsDNA unwinding.

KW - ADHESION

KW - BOND RUPTURE FORCES

KW - DUPLEX

KW - DYNAMIC FORCE SPECTROSCOPY

KW - ENERGY LANDSCAPES

KW - EVENTS

KW - MICROSCOPY

KW - MISMATCHES

KW - STRANDS

KW - STRENGTH

UR - http://www.scopus.com/inward/record.url?scp=85043488434&partnerID=8YFLogxK

U2 - 10.1021/acsomega.7b01815

DO - 10.1021/acsomega.7b01815

M3 - Article

C2 - 30023851

AN - SCOPUS:85043488434

VL - 3

SP - 2793

EP - 2797

JO - ACS Omega

JF - ACS Omega

SN - 2470-1343

IS - 3

ER -

ID: 10453802