TY - JOUR

T1 - Detection of causative agents of tick-borne rickettsioses in Western Siberia, Russia

T2 - identification of Rickettsia raoultii and Rickettsia sibirica DNA in clinical samples

AU - Igolkina, Y.

AU - Krasnova, E.

AU - Rar, V.

AU - Savelieva, M.

AU - Epikhina, T.

AU - Tikunov, A.

AU - Khokhlova, N.

AU - Provorova, V.

AU - Tikunova, N.

N1 - Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Objectives: The main causative agent of tick-borne rickettsioses in Siberia is considered to be Rickettsia sibirica; however, only a few cases have been genetically confirmed. Other pathogenic species of Rickettsia have been detected in ixodid ticks in Western Siberia. The aim of this study was to detect the aetiological agents of tick-borne rickettsioses in Western Siberia and compare their clinical manifestations. Methods: A total of 273 blood and 44 cerebrospinal fluid (CSF) samples from 273 patients hospitalized because of tick-transmitted infection in April–September 2016 were examined for the presence of Rickettsia spp., using nested PCR with subsequent sequencing. Results: DNA of Rickettsia spp. was found in samples from 10 patients. The gltA gene fragment sequence analysis revealed R. sibirica DNA in seven patients (blood samples) and Rickettsia raoultii DNA in three patients (two blood and one CSF sample). Most patients infected with R. sibirica showed typical clinical symptoms, including high-grade fever (38.9–39.5°С), myalgia, rash, eschar at the site of the tick bite, and elevated levels of serum aminotransferases. In contrast, patients infected with R. raoultii showed nonspecific symptoms with short-term fever (37.2–37.7°С); one patient had a short episode of meningeal syndrome. Conclusions: We report the first finding of R. raoultii DNA in clinical samples from Russian patients. The clinical manifestations of this rickettsiosis were nonspecific and differed from those caused by R. sibirica.

AB - Objectives: The main causative agent of tick-borne rickettsioses in Siberia is considered to be Rickettsia sibirica; however, only a few cases have been genetically confirmed. Other pathogenic species of Rickettsia have been detected in ixodid ticks in Western Siberia. The aim of this study was to detect the aetiological agents of tick-borne rickettsioses in Western Siberia and compare their clinical manifestations. Methods: A total of 273 blood and 44 cerebrospinal fluid (CSF) samples from 273 patients hospitalized because of tick-transmitted infection in April–September 2016 were examined for the presence of Rickettsia spp., using nested PCR with subsequent sequencing. Results: DNA of Rickettsia spp. was found in samples from 10 patients. The gltA gene fragment sequence analysis revealed R. sibirica DNA in seven patients (blood samples) and Rickettsia raoultii DNA in three patients (two blood and one CSF sample). Most patients infected with R. sibirica showed typical clinical symptoms, including high-grade fever (38.9–39.5°С), myalgia, rash, eschar at the site of the tick bite, and elevated levels of serum aminotransferases. In contrast, patients infected with R. raoultii showed nonspecific symptoms with short-term fever (37.2–37.7°С); one patient had a short episode of meningeal syndrome. Conclusions: We report the first finding of R. raoultii DNA in clinical samples from Russian patients. The clinical manifestations of this rickettsiosis were nonspecific and differed from those caused by R. sibirica.

KW - Clinical manifestations

KW - Ixodid ticks

KW - Rickettsia raoultii

KW - Rickettsia sibirica

KW - Rickettsioses

KW - HUMANS

KW - SLOVACA

KW - REGION

KW - Rickettsia Infections/microbiology

KW - Humans

KW - Phylogeny

KW - Rickettsia/genetics

KW - Sequence Analysis, DNA

KW - Polymerase Chain Reaction

KW - Siberia/epidemiology

KW - DNA, Bacterial/genetics

KW - Tick-Borne Diseases/microbiology

UR - http://www.scopus.com/inward/record.url?scp=85025804764&partnerID=8YFLogxK

U2 - 10.1016/j.cmi.2017.06.003

DO - 10.1016/j.cmi.2017.06.003

M3 - Article

C2 - 28606645

AN - SCOPUS:85025804764

VL - 24

SP - 199.e9-199.e12

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

SN - 1198-743X

IS - 2

ER -