Research output: Contribution to journal › Article › peer-review
Detection of causative agents of tick-borne rickettsioses in Western Siberia, Russia : identification of Rickettsia raoultii and Rickettsia sibirica DNA in clinical samples. / Igolkina, Y.; Krasnova, E.; Rar, V. et al.
In: Clinical Microbiology and Infection, Vol. 24, No. 2, 01.02.2018, p. 199.e9-199.e12.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Detection of causative agents of tick-borne rickettsioses in Western Siberia, Russia
T2 - identification of Rickettsia raoultii and Rickettsia sibirica DNA in clinical samples
AU - Igolkina, Y.
AU - Krasnova, E.
AU - Rar, V.
AU - Savelieva, M.
AU - Epikhina, T.
AU - Tikunov, A.
AU - Khokhlova, N.
AU - Provorova, V.
AU - Tikunova, N.
N1 - Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
PY - 2018/2/1
Y1 - 2018/2/1
N2 - Objectives: The main causative agent of tick-borne rickettsioses in Siberia is considered to be Rickettsia sibirica; however, only a few cases have been genetically confirmed. Other pathogenic species of Rickettsia have been detected in ixodid ticks in Western Siberia. The aim of this study was to detect the aetiological agents of tick-borne rickettsioses in Western Siberia and compare their clinical manifestations. Methods: A total of 273 blood and 44 cerebrospinal fluid (CSF) samples from 273 patients hospitalized because of tick-transmitted infection in April–September 2016 were examined for the presence of Rickettsia spp., using nested PCR with subsequent sequencing. Results: DNA of Rickettsia spp. was found in samples from 10 patients. The gltA gene fragment sequence analysis revealed R. sibirica DNA in seven patients (blood samples) and Rickettsia raoultii DNA in three patients (two blood and one CSF sample). Most patients infected with R. sibirica showed typical clinical symptoms, including high-grade fever (38.9–39.5°С), myalgia, rash, eschar at the site of the tick bite, and elevated levels of serum aminotransferases. In contrast, patients infected with R. raoultii showed nonspecific symptoms with short-term fever (37.2–37.7°С); one patient had a short episode of meningeal syndrome. Conclusions: We report the first finding of R. raoultii DNA in clinical samples from Russian patients. The clinical manifestations of this rickettsiosis were nonspecific and differed from those caused by R. sibirica.
AB - Objectives: The main causative agent of tick-borne rickettsioses in Siberia is considered to be Rickettsia sibirica; however, only a few cases have been genetically confirmed. Other pathogenic species of Rickettsia have been detected in ixodid ticks in Western Siberia. The aim of this study was to detect the aetiological agents of tick-borne rickettsioses in Western Siberia and compare their clinical manifestations. Methods: A total of 273 blood and 44 cerebrospinal fluid (CSF) samples from 273 patients hospitalized because of tick-transmitted infection in April–September 2016 were examined for the presence of Rickettsia spp., using nested PCR with subsequent sequencing. Results: DNA of Rickettsia spp. was found in samples from 10 patients. The gltA gene fragment sequence analysis revealed R. sibirica DNA in seven patients (blood samples) and Rickettsia raoultii DNA in three patients (two blood and one CSF sample). Most patients infected with R. sibirica showed typical clinical symptoms, including high-grade fever (38.9–39.5°С), myalgia, rash, eschar at the site of the tick bite, and elevated levels of serum aminotransferases. In contrast, patients infected with R. raoultii showed nonspecific symptoms with short-term fever (37.2–37.7°С); one patient had a short episode of meningeal syndrome. Conclusions: We report the first finding of R. raoultii DNA in clinical samples from Russian patients. The clinical manifestations of this rickettsiosis were nonspecific and differed from those caused by R. sibirica.
KW - Clinical manifestations
KW - Ixodid ticks
KW - Rickettsia raoultii
KW - Rickettsia sibirica
KW - Rickettsioses
KW - HUMANS
KW - SLOVACA
KW - REGION
KW - Rickettsia Infections/microbiology
KW - Humans
KW - Phylogeny
KW - Rickettsia/genetics
KW - Sequence Analysis, DNA
KW - Polymerase Chain Reaction
KW - Siberia/epidemiology
KW - DNA, Bacterial/genetics
KW - Tick-Borne Diseases/microbiology
UR - http://www.scopus.com/inward/record.url?scp=85025804764&partnerID=8YFLogxK
U2 - 10.1016/j.cmi.2017.06.003
DO - 10.1016/j.cmi.2017.06.003
M3 - Article
C2 - 28606645
AN - SCOPUS:85025804764
VL - 24
SP - 199.e9-199.e12
JO - Clinical Microbiology and Infection
JF - Clinical Microbiology and Infection
SN - 1198-743X
IS - 2
ER -
ID: 10070051