Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Correlated target search by uracil-DNA glycosylase in the presence of bulky adducts and DNA-binding ligands. / Mechetin, G. V.; Dyatlova, E. A.; Sinyakov, A. N. и др.
в: Russian Journal of Bioorganic Chemistry, Том 43, № 1, 01.01.2017, стр. 23-28.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Correlated target search by uracil-DNA glycosylase in the presence of bulky adducts and DNA-binding ligands
AU - Mechetin, G. V.
AU - Dyatlova, E. A.
AU - Sinyakov, A. N.
AU - Ryabinin, V. A.
AU - Vorobjev, P. E.
AU - Zharkov, D. O.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Many proteins specific for rare targets in DNA, such as transcription factors, restriction endonucleases, and DNA repair enzymes, search for their targets by one-dimensional diffusion along DNA. One of these proteins is uracil-DNA glycosylase (Ung), which excises the uracil bases formed by rare events of cytosine deamination. We have studied the ability of Ung to move along DNA with its path hindered by bulky DNA covalent adducts (fluorescein) or ligands blocking the major or minor DNA groove. The fluorescein adduct strongly inhibits translocation only along double-stranded DNA, whereas noncovalently bound ligands partly inhibit DNA cleavage but barely affect translocation. The ability of uracil-DNA glycosylase to search for its targets in the presence of molecules competing for DNA binding may be important for DNA repair in the intracellular environment.
AB - Many proteins specific for rare targets in DNA, such as transcription factors, restriction endonucleases, and DNA repair enzymes, search for their targets by one-dimensional diffusion along DNA. One of these proteins is uracil-DNA glycosylase (Ung), which excises the uracil bases formed by rare events of cytosine deamination. We have studied the ability of Ung to move along DNA with its path hindered by bulky DNA covalent adducts (fluorescein) or ligands blocking the major or minor DNA groove. The fluorescein adduct strongly inhibits translocation only along double-stranded DNA, whereas noncovalently bound ligands partly inhibit DNA cleavage but barely affect translocation. The ability of uracil-DNA glycosylase to search for its targets in the presence of molecules competing for DNA binding may be important for DNA repair in the intracellular environment.
KW - covalent DNA adducts
KW - DNA target search
KW - minor groove binders
KW - triplex-forming oligonucleotides
KW - uracil-DNA glycosylase
KW - TRANSLOCATION
KW - MECHANISM
KW - RECOGNITION
KW - ESCHERICHIA-COLI
KW - CLEAVAGE
KW - FACILITATED DIFFUSION
KW - REPAIR
KW - LESION SEARCH
UR - http://www.scopus.com/inward/record.url?scp=85012923846&partnerID=8YFLogxK
U2 - 10.1134/S106816201606008X
DO - 10.1134/S106816201606008X
M3 - Article
AN - SCOPUS:85012923846
VL - 43
SP - 23
EP - 28
JO - Russian Journal of Bioorganic Chemistry
JF - Russian Journal of Bioorganic Chemistry
SN - 1068-1620
IS - 1
ER -
ID: 8672909