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Correlated target search by uracil-DNA glycosylase in the presence of bulky adducts and DNA-binding ligands. / Mechetin, G. V.; Dyatlova, E. A.; Sinyakov, A. N. et al.

In: Russian Journal of Bioorganic Chemistry, Vol. 43, No. 1, 01.01.2017, p. 23-28.

Research output: Contribution to journal › Article › peer-review

Harvard

Mechetin, GV, Dyatlova, EA, Sinyakov, AN, Ryabinin, VA, Vorobjev, PE & Zharkov, DO 2017, 'Correlated target search by uracil-DNA glycosylase in the presence of bulky adducts and DNA-binding ligands', Russian Journal of Bioorganic Chemistry, vol. 43, no. 1, pp. 23-28. https://doi.org/10.1134/S106816201606008X

APA

Mechetin, G. V., Dyatlova, E. A., Sinyakov, A. N., Ryabinin, V. A., Vorobjev, P. E., & Zharkov, D. O. (2017). Correlated target search by uracil-DNA glycosylase in the presence of bulky adducts and DNA-binding ligands. Russian Journal of Bioorganic Chemistry, 43(1), 23-28. https://doi.org/10.1134/S106816201606008X

Vancouver

Mechetin GV, Dyatlova EA, Sinyakov AN, Ryabinin VA, Vorobjev PE, Zharkov DO. Correlated target search by uracil-DNA glycosylase in the presence of bulky adducts and DNA-binding ligands. Russian Journal of Bioorganic Chemistry. 2017 Jan 1;43(1):23-28. doi: 10.1134/S106816201606008X

Author

Mechetin, G. V. ; Dyatlova, E. A. ; Sinyakov, A. N. et al. / Correlated target search by uracil-DNA glycosylase in the presence of bulky adducts and DNA-binding ligands. In: Russian Journal of Bioorganic Chemistry. 2017 ; Vol. 43, No. 1. pp. 23-28.

BibTeX

@article{e6bb37a74b214734b4cd046d27138fa8,
title = "Correlated target search by uracil-DNA glycosylase in the presence of bulky adducts and DNA-binding ligands",
abstract = "Many proteins specific for rare targets in DNA, such as transcription factors, restriction endonucleases, and DNA repair enzymes, search for their targets by one-dimensional diffusion along DNA. One of these proteins is uracil-DNA glycosylase (Ung), which excises the uracil bases formed by rare events of cytosine deamination. We have studied the ability of Ung to move along DNA with its path hindered by bulky DNA covalent adducts (fluorescein) or ligands blocking the major or minor DNA groove. The fluorescein adduct strongly inhibits translocation only along double-stranded DNA, whereas noncovalently bound ligands partly inhibit DNA cleavage but barely affect translocation. The ability of uracil-DNA glycosylase to search for its targets in the presence of molecules competing for DNA binding may be important for DNA repair in the intracellular environment.",
keywords = "covalent DNA adducts, DNA target search, minor groove binders, triplex-forming oligonucleotides, uracil-DNA glycosylase, TRANSLOCATION, MECHANISM, RECOGNITION, ESCHERICHIA-COLI, CLEAVAGE, FACILITATED DIFFUSION, REPAIR, LESION SEARCH",
author = "Mechetin, {G. V.} and Dyatlova, {E. A.} and Sinyakov, {A. N.} and Ryabinin, {V. A.} and Vorobjev, {P. E.} and Zharkov, {D. O.}",
year = "2017",
month = jan,
day = "1",
doi = "10.1134/S106816201606008X",
language = "English",
volume = "43",
pages = "23--28",
journal = "Russian Journal of Bioorganic Chemistry",
issn = "1068-1620",
publisher = "MAIK NAUKA/INTERPERIODICA/SPRINGER",
number = "1",

}

RIS

TY - JOUR

T1 - Correlated target search by uracil-DNA glycosylase in the presence of bulky adducts and DNA-binding ligands

AU - Mechetin, G. V.

AU - Dyatlova, E. A.

AU - Sinyakov, A. N.

AU - Ryabinin, V. A.

AU - Vorobjev, P. E.

AU - Zharkov, D. O.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Many proteins specific for rare targets in DNA, such as transcription factors, restriction endonucleases, and DNA repair enzymes, search for their targets by one-dimensional diffusion along DNA. One of these proteins is uracil-DNA glycosylase (Ung), which excises the uracil bases formed by rare events of cytosine deamination. We have studied the ability of Ung to move along DNA with its path hindered by bulky DNA covalent adducts (fluorescein) or ligands blocking the major or minor DNA groove. The fluorescein adduct strongly inhibits translocation only along double-stranded DNA, whereas noncovalently bound ligands partly inhibit DNA cleavage but barely affect translocation. The ability of uracil-DNA glycosylase to search for its targets in the presence of molecules competing for DNA binding may be important for DNA repair in the intracellular environment.

AB - Many proteins specific for rare targets in DNA, such as transcription factors, restriction endonucleases, and DNA repair enzymes, search for their targets by one-dimensional diffusion along DNA. One of these proteins is uracil-DNA glycosylase (Ung), which excises the uracil bases formed by rare events of cytosine deamination. We have studied the ability of Ung to move along DNA with its path hindered by bulky DNA covalent adducts (fluorescein) or ligands blocking the major or minor DNA groove. The fluorescein adduct strongly inhibits translocation only along double-stranded DNA, whereas noncovalently bound ligands partly inhibit DNA cleavage but barely affect translocation. The ability of uracil-DNA glycosylase to search for its targets in the presence of molecules competing for DNA binding may be important for DNA repair in the intracellular environment.

KW - covalent DNA adducts

KW - DNA target search

KW - minor groove binders

KW - triplex-forming oligonucleotides

KW - uracil-DNA glycosylase

KW - TRANSLOCATION

KW - MECHANISM

KW - RECOGNITION

KW - ESCHERICHIA-COLI

KW - CLEAVAGE

KW - FACILITATED DIFFUSION

KW - REPAIR

KW - LESION SEARCH

UR - http://www.scopus.com/inward/record.url?scp=85012923846&partnerID=8YFLogxK

U2 - 10.1134/S106816201606008X

DO - 10.1134/S106816201606008X

M3 - Article

AN - SCOPUS:85012923846

VL - 43

SP - 23

EP - 28

JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

SN - 1068-1620

IS - 1

ER -

ID: 8672909