TY - JOUR

T1 - Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius)

T2 - 5000RAD and 15000RAD

AU - Perelman, Polina L.

AU - Pichler, Rudolf

AU - Gaggl, Anna

AU - Larkin, Denis M.

AU - Raudsepp, Terje

AU - Alshanbari, Fahad

AU - Holl, Heather M.

AU - Brooks, Samantha A.

AU - Burger, Pamela A.

AU - Periasamy, Kathiravan

N1 - Publisher Copyright: © 2018 The Author(s).

PY - 2018/1/31

Y1 - 2018/1/31

N2 - The availability of genomic resources including linkage information for camelids has been very limited. Here, we describe the construction of a set of two radiation hybrid (RH) panels (5000RAD and 15000RAD) for the dromedary (Camelus dromedarius) as a permanent genetic resource for camel genome researchers worldwide. For the 5000RAD panel, a total of 245 female camel-hamster radiation hybrid clones were collected, of which 186 were screened with 44 custom designed marker loci distributed throughout camel genome. The overall mean retention frequency (RF) of the final set of 93 hybrids was 47.7%. For the 15000RAD panel, 238 male dromedary-hamster radiation hybrid clones were collected, of which 93 were tested using 44 PCR markers. The final set of 90 clones had a mean RF of 39.9%. This 15000RAD panel is an important high-resolution complement to the main 5000RAD panel and an indispensable tool for resolving complex genomic regions. This valuable genetic resource of dromedary RH panels is expected to be instrumental for constructing a high resolution camel genome map. Construction of the set of RH panels is essential step toward chromosome level reference quality genome assembly that is critical for advancing camelid genomics and the development of custom genomic tools.

AB - The availability of genomic resources including linkage information for camelids has been very limited. Here, we describe the construction of a set of two radiation hybrid (RH) panels (5000RAD and 15000RAD) for the dromedary (Camelus dromedarius) as a permanent genetic resource for camel genome researchers worldwide. For the 5000RAD panel, a total of 245 female camel-hamster radiation hybrid clones were collected, of which 186 were screened with 44 custom designed marker loci distributed throughout camel genome. The overall mean retention frequency (RF) of the final set of 93 hybrids was 47.7%. For the 15000RAD panel, 238 male dromedary-hamster radiation hybrid clones were collected, of which 93 were tested using 44 PCR markers. The final set of 90 clones had a mean RF of 39.9%. This 15000RAD panel is an important high-resolution complement to the main 5000RAD panel and an indispensable tool for resolving complex genomic regions. This valuable genetic resource of dromedary RH panels is expected to be instrumental for constructing a high resolution camel genome map. Construction of the set of RH panels is essential step toward chromosome level reference quality genome assembly that is critical for advancing camelid genomics and the development of custom genomic tools.

KW - Animals

KW - Camelus/genetics

KW - Cricetinae

KW - DNA/genetics

KW - Female

KW - Genome

KW - Radiation Hybrid Mapping

KW - HORSE

KW - CATTLE

KW - COMPARATIVE MAP

KW - PIG

KW - CHROMOSOME

KW - ASSEMBLIES

KW - SEQUENCE

KW - GENERATION

KW - GOAT

UR - http://www.scopus.com/inward/record.url?scp=85041322600&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-20223-5

DO - 10.1038/s41598-018-20223-5

M3 - Article

C2 - 29386528

AN - SCOPUS:85041322600

VL - 8

SP - 1982

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 1982

ER -