Research output: Contribution to journal › Article › peer-review
Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius) : 5000RAD and 15000RAD. / Perelman, Polina L.; Pichler, Rudolf; Gaggl, Anna et al.
In: Scientific Reports, Vol. 8, No. 1, 1982, 31.01.2018, p. 1982.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius)
T2 - 5000RAD and 15000RAD
AU - Perelman, Polina L.
AU - Pichler, Rudolf
AU - Gaggl, Anna
AU - Larkin, Denis M.
AU - Raudsepp, Terje
AU - Alshanbari, Fahad
AU - Holl, Heather M.
AU - Brooks, Samantha A.
AU - Burger, Pamela A.
AU - Periasamy, Kathiravan
N1 - Publisher Copyright: © 2018 The Author(s).
PY - 2018/1/31
Y1 - 2018/1/31
N2 - The availability of genomic resources including linkage information for camelids has been very limited. Here, we describe the construction of a set of two radiation hybrid (RH) panels (5000RAD and 15000RAD) for the dromedary (Camelus dromedarius) as a permanent genetic resource for camel genome researchers worldwide. For the 5000RAD panel, a total of 245 female camel-hamster radiation hybrid clones were collected, of which 186 were screened with 44 custom designed marker loci distributed throughout camel genome. The overall mean retention frequency (RF) of the final set of 93 hybrids was 47.7%. For the 15000RAD panel, 238 male dromedary-hamster radiation hybrid clones were collected, of which 93 were tested using 44 PCR markers. The final set of 90 clones had a mean RF of 39.9%. This 15000RAD panel is an important high-resolution complement to the main 5000RAD panel and an indispensable tool for resolving complex genomic regions. This valuable genetic resource of dromedary RH panels is expected to be instrumental for constructing a high resolution camel genome map. Construction of the set of RH panels is essential step toward chromosome level reference quality genome assembly that is critical for advancing camelid genomics and the development of custom genomic tools.
AB - The availability of genomic resources including linkage information for camelids has been very limited. Here, we describe the construction of a set of two radiation hybrid (RH) panels (5000RAD and 15000RAD) for the dromedary (Camelus dromedarius) as a permanent genetic resource for camel genome researchers worldwide. For the 5000RAD panel, a total of 245 female camel-hamster radiation hybrid clones were collected, of which 186 were screened with 44 custom designed marker loci distributed throughout camel genome. The overall mean retention frequency (RF) of the final set of 93 hybrids was 47.7%. For the 15000RAD panel, 238 male dromedary-hamster radiation hybrid clones were collected, of which 93 were tested using 44 PCR markers. The final set of 90 clones had a mean RF of 39.9%. This 15000RAD panel is an important high-resolution complement to the main 5000RAD panel and an indispensable tool for resolving complex genomic regions. This valuable genetic resource of dromedary RH panels is expected to be instrumental for constructing a high resolution camel genome map. Construction of the set of RH panels is essential step toward chromosome level reference quality genome assembly that is critical for advancing camelid genomics and the development of custom genomic tools.
KW - Animals
KW - Camelus/genetics
KW - Cricetinae
KW - DNA/genetics
KW - Female
KW - Genome
KW - Radiation Hybrid Mapping
KW - HORSE
KW - CATTLE
KW - COMPARATIVE MAP
KW - PIG
KW - CHROMOSOME
KW - ASSEMBLIES
KW - SEQUENCE
KW - GENERATION
KW - GOAT
UR - http://www.scopus.com/inward/record.url?scp=85041322600&partnerID=8YFLogxK
U2 - 10.1038/s41598-018-20223-5
DO - 10.1038/s41598-018-20223-5
M3 - Article
C2 - 29386528
AN - SCOPUS:85041322600
VL - 8
SP - 1982
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 1982
ER -
ID: 12078957